[Evaluation of Indicators of Postejaculation Maturation of Spermatozoa of Bos taurus Using a Chlortetracycline Test].

Postejaculation maturation of spermatozoa (capacitation and acrosomal exocytosis) in bovine sperm was assessed using the method of staining with chlortetracycline and inhibitor analysis to identify the transition ways of calcium of intracellular depots under the influence of highly dispersed silica. It was shown that highly dispersed silica in a concentration of 0.001% stimulates capacitation but has no impact on the acrosome exocytosis in bovine sperm.

[Effect of vitrification on the functioning of the structural elements of the cytoskeleton in porcine oocytes].

On the basis of inhibition analysis using inhibitors of protein kinase A and polymerization of microfilament under the influence oftheophylline, GDP and GTP have studied the release of Ca2+ from intracellular stores (ryanodin- and inositol-1 ,4,5-trisphosphate) of native and devitrified porcine oocytes. It is found that theophylline, as well as GDP stimulate the release of Ca2+ from intracellular stores in the native and devitrified oocytes, but a further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP was observed only in native oocytes. Inhibitors of protein kinase A and polymerization of microfilaments in native oocytes have a negative impact on the further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP.

[Clinical efficacy of functional nutrition in patients with gastrointestinal disorders].

Aim: To identify micronutrient deficiencies in patients with functional bowel diseases (FBD) and to reveal their correction with functional foods and probiotics.

Subjects And Methods: The health status was evaluated in 90 patients aged 18 to 67 years with FBD. All the patients were randomized into 3 groups according to the treatment regimen.

[Solved and unsolved problems of nutritional support in surgical gastroenterology].

The article has revealed the relevance of nutriciology as a science, classification and types of nutritional support. Nutritional support--diagnostics and the metabolic correction of arising violations of various kinds of homeostasis, aimed at ensuring the functioning of the systems of protein-energy synthesis and intermediate exchange of nutrients, vitamins, macro--and microelements, or a temporary replacement therapy of their failure. The notions of "lack of food", "artificial" food, protein-energy insufficiency with the exception of the syndromes of malabsorption, alimentary anemia, the effects of protein-energy malnutrition, consumptive disease, starvation have been interpreted.

[Identification of mechanisms of the calcium signaling at influence of estradiol on porcine oocytes, stimulated by theophillin and somatotropin].

Through the use of inhibitory analysis by using the fluorescent probe chlortetracycline examined the effects of estradiol on the mobilization of Ca2+ from intracellular stores of porcine oocytes stimulated by growth hormone and theophylline. It is shown that somatotropin or theophylline stimulates the mobilization of Ca2+ from intracellular stores of oocytes in control group, while their combined action does not lead to an additional exit of Ca2+ from intracellular stores. Inhibitor of protein kinase C had no effect on the release of Ca2+--from oocyte stimulated by growth hormone or theophylline.

[Immunological criteria for evaluation of nutritional support in ulcerative colitis].

The article presents the data on the assessment of nutritional status, taking into account humoral immunologic criteria of patients with ulcerative colitis.

[Ca2+ exit from intracellular stores of growing and fully grown native and devitrified porcine oocytes].

The exit of Ca2+ from intracellular stores in growing and fully grown native and devitrified porcine oocytes stimulated by somatotropin and GTP was investigated using fluorescent dye chlortetracycline. In native as well as in the devitrified porcines oocytes, in their fully grown phase, joint action of somatotropin and GTP stimulated additional freeing of Ca2+ from intracellular stores, but during subsequent processing of the cell with nocodazole (a polymerization inhibitor of microtubules), additional exit of calcium did not occur. In the growing phase of native oocytes during the joint acting of somatotropin and GTP additional exit for Ca2+ from the intracellular stores did not occur.

[Strongylids (Nematoda: Strongylidae) of domestic horses in Ukraine: modern state of Fauna and structure of the parasite community].

Species composition and structure of the strongylid community was studies on helminthological material collected from 162 domestic horses from 11 regions of Ukraine by the in vivo method. Animals were treated with anthelmintic drug "Univerm" (0.2% aversectin C, Russia).

[Nutrition support of patients after extensive intestinal resections].

[Formula see text], their profile can be one of markers, characterizing activity of colon microflora at the consequences of the vast resections of bowels, and to determine setting of preparations of medicamental correction in the structure of nutritional therapy by siping compositions.

[Identification of signal transduction pathway in fresh and vitrified porcine oocytes].

Signal transduction pathway under the influence of somatotropin have been identified basis on the analysis of Ca2+ release from intracellular stores of fresh and vitrified porcine oocytes using inhibitory analysis. Somatotropin and GTP individually stimulated Ca2+ release from intracellular stores. The joint action of somatotropin and GTP activated additional Ca2+ release from intracellular stores both in fresh and vitrified porcine oocytes.

[Ca2+ release from intracellular stores of pig oocytes during different stages of growth].

Ca2+ release from intracellular stores of pig oocytes was investigated using the Ca(2+)-sensitive fluorescent dye chlorotetracycline. Oocytes were divided into growing ones and those that completed their growth using brilliant cresyl clue (BCB) staining. The stained oocytes (BCB "+") were determined as the ones that completed their growth, while the stainless ones (BCB "-") were determined as those in the final stages of growth.

[Postgastrectomy syndromes in therapeutic practice ].

Aim: To specify policy of nutritive support late after radical gastric resection.

Material And Methods: Patients with postgastroresectional dystrophy were examined using standard techniques and estimation of intestinal electric activity (registration of body surface biopotentials on Conan-M myngograph).

Results: Frequency-amplitude parameters of the intestine serve the basis for choice of mixtures for enteral correction.

[Effect of testosterone in Ca2+ mobilization in oocytes and porcine granulose stimulated by prolactin and theophylline].

Influence of testosterone in Ca2+ exchange in oocytes and porcine granulose stimulated by prolactin and theophylline was investigated using fluorescent dye chlortetracycline. It was shown that prolactin and theophylline separately have not affected on the Ca2+ exit from intracellular stores of oocytes. Prolactin and theophylline in common stimulate exit of Ca2+ from intracellular stores in oocytes, which decreased at presence of inhibitor of protein kinase A.

[Frequency and prophylaxis of the most generalized diseases among young men of preinduction age].

The article presents the analyze of indexes of morbidity among young men of preinduction age in Orenburg region. Was given a valuation of level of risk of forming of diseases. Were shown causes of deficit of weight, growth of pathology of locomotor apparatus and psychic diseases among young men of preinduction age.

[Effect of estradiol on fluctuation of calcium in swine granulose cells stimulated by theophylline and prolactin].

Effect of estradiol on Ca2+ exit from intracellular stores in swine granulose cells activated by theophylline and prolactin is investigated with using fluorescent Ca2+ sensitive probe chlortetracycline. It was shown that prolactin and theophylline stimulated Ca2+ exit from intracellular stores. In absence of estradiol if we treatment of oocytes by theophylline and prolactin together we have not found influence at Ca2+ exit from intracellular stores.

[Present status of clinical and fundamental threpsology].

A good nutrition status is one of the impotant prerequisites of good health. Malnutrition in hospital leads to increased complication, morbidity and mortality rates. Whenever the gastrointestinal tract cannot fulfil its function or must be rested (during intestinal desease), parenteral administration of nutrients (or simultative parenteral and enteral alimitation) is necessary.

[Antigenicity and B-epitope mapping of hepatitis C virus envelope protein E2].

Immunogenicity for laboratory animals (rabbits and mice) of the whole hepatitis C virus envelope proteins and their conserved as well as hypervariable HVR1 sites has been investigated. Rabbit immune responses to HCV envelope proteins (both single E2 and E1E2 heterodimer) were shown to be much more efficient than murine immune responses. Upon the immunization of the rabbit with E2 protein, antibodies to several highly conserved linear B-epitopes of this protein as well as to the N-terminal fragment of the hypervariable region HVRI were formed.

[Effect of estradiol on Ca2+ release from intracellular stores in porcine oocytes stimulated by prolactin, theophylline, or guanosine triphosphate].

The interaction between prolactin and theophylline as well as between prolactin and guanosine triphosphate during Ca2+ release from intracellular stores of estradiol-treated porcine oocytes isolated from the ovary at the stage of follicular growth were studied using fluorescent Ca(2+)-sensitive probe chlortetracycline. In the absence of estradiol, prolactin or theophylline induced Ca2+ release from intracellular stores; however, no increase in Ca2+ release was observed after their combined action. Conversely, Ca2+ release from intracellular stores increased only after the combined exposure to prolactin and theophylline in the the presence of estradiol.

[Parenteral nutrition in treatment of patients with syndrome of short bowel].

If the gut is too short parenteral feeding becomes the only option. As clinicians interested in this field, we must continue to inform others about what standarts may be set in different patients. Nowadays the enteral nutrition is also used in the scheem of the treatment such patients.

[Metabolic activity of intestinal microflora and its role in determination of nutritional support tactic after gastrectomy].

Nutrition recommendations in symptoms of postgastrectomy syndroms is very different. The calculation of regimen using the parenteral and enteral alimentation may be established in the control test of definition of fecal short-chain fatty acids and microflora in such patients. Probiotics attach to the intestinal wall and protect it from the attacks of pathologic microorganisms.

[Enteral nutrition in the therapy of gastrointestinal diseases (according to materials of the European Association of Parenteral and Enteral Nutrition)].

The present article gives evidence-based recommendations for the indication, application and type of formula of enteral nutrition (EN) (oral nutrition supplements (ONS) or tube feeding (TF)) in patients with Crohn's disease (CD), ulcerative colitis (UC), short bowel syndrome (SBS), acute and chronic pancreatitis, alcoholic steatogepatitis and cirrosis. ONS and/or TF in addition to normal food is indicates in undernourished patients with CD or UC to improve nutritional status. In active CD EN is the first line therapy in children and should be used as sole therapy in adults mainly when treatment with corticosteroids is not feasible.

[Influence of ryanodine and inositol trisphosphate receptors inhibitions on Ca2+ exit from intracellular stores of porcine oocytes stimulated by prolactin and GTP].

The influence of ryanodine and inositol triphosphate receptors inhibitors on Ca2+ exit from intracellular stores of porcine oocytes stimulated by prolactin and GTP was investigated using fluorescent dye chlortetracycline. Porcine oocytes were isolated from ovaries with yellow body. Ca2+ exit from intracellular stores of porcine oocytes activated by prolactin (5 and 50 ng/ml) in calcium free medium was decreased after treatment of oocytes by heparin (inhibitor of inositol triphosphate receptors) and was not changed after treatment of oocytes by ruthenium red (inhibitor of ryanodine receptors).