[Elimination of late endoleak after endovascular repair of a dissecting thoracoabdominal aortic aneurysm].

Described herein is a clinical case report regarding treatment of a 70-year-old male patient presenting with a late complication following endoprosthetic repair for a Stanford type B dissecting thoracic aortic aneurysm. The man was admitted to our hospital for persistent type IIb endoleak and an increased diameter of the aorta in its thoracic and thoracoabdominal portions. Two years previously, he had endured endoprosthetic repair of the thoracic aorta.

[Technologies and control of cleanness of the rooms in the blood center].

To prevent bacterial contamination of blood transfusion media, the following three types of rooms in blood service institutions should meet especially high requirements for cleanness: 1. Box and its foreroom (or room equipped with laminar cabinet) for open-air preparation of washed red blood cells or other blood components. 2.

[The hemotransfusion therapy rendered to the wounded during antiterrorist operation].

The blood transfusion therapy volume and structure at various stages of medical care in the wounded at antiterrorist operation on Northern Caucasus in 1999-2002 are discussed. The differences in the blood transfusion therapy structure at various stages of the military operation are established. In specialized medical care the use of blood components and products reached 86.

[Characteristics of the provision of hemotransfusion materials to the wounded and patients during antiterrorist operation].

The authors analyze the experience of organization of the wounded and patient provision with hemotransfusion materials to render assistance during antiterrorist operation in Republic of Dagestan and Republic of Chechnya in 1999-2001. It is shown that supply of military treatment-and-prophylactic institutions deployed in the zone of military conflict, of specialized hospitals with hemotransfusion materials was the following: with blood preparations (at the expense of centralized deliveries), with blood components (91.8%, at the expense of centralized deliveries form the district blood transfusion station).

[The past, present and future of the blood service of the Armed Forces].

Creation of the civil blood service permitted to supply the military treatment-and-prophylactic institutions with blood, to draw up the plan of support in the event of hostilities. The blood service of the USSR (Russia) Armed Forces originates from 1962 when the blood transfusion stations (BTS) were created in the military districts and navy. The departments of blood transfusion (DBT) were formed in the central hospitals and Military Medical Academy and the stations of blood storage and transfusion not in the regular staff were organized in the garrison hospitals.

[Restriction endonucleases from various bacterial strains exhibit an ice-nucleating activity].

Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain.

[Improvement in cryopreservation of blood platelets].

The first Russian solution Krimolit designed for cryopreservation of platelet concentrates down -196 degrees C was clinically tested. The therapeutical efficacy of the cryopreserved platelets was evaluated on the basis of clinical and laboratory monitoring of transfusions in 20 cancer hematological patients. They were found to have the same therapeutical action as fresh cells.

[Utilization of detoxification products of yperite-lewisite mixtures].

An approach to developing an ecologically safe technology for disposal of yperite-lewisite mixtures has been developed. The technology includes three sequential stages: (1) detoxification by alkaline hydrolysis (resulting in the loss of toxic properties); (2) electrochemical treatment of detoxification products (electrocoagulation, to eliminate arsenic salts, and electrochemical oxidation, to convert all organic components into bioutilizable matter); and (3) degradation of the compounds obtained at the two preceding stages by a bred microbial association.

[Scientific principles of complex ecologically-safe technology of mustard gas destruction].

The principles of complex, ecologically-safe technology for the destruction of battle gas mustard were worked out. This technology was based on the reaction alkaline detoxication of mustard; the major component of reaction mixture obtained after detoxication was thiodiglycol. Thorough thiodiglycol mineralization was achieved by electrochemical treatment.

[Evidence of the existence of hemolysin II from Bacillus cereus: cloning the genetic determinant of hemolysin II].

The hemolysin genetic determinant distinct from cereolysin AB genetic determinant (lecithinase and sphingomyelinase genes) has been cloned in Escherichia coli and Bacillus subtilis cells as an EcoRI fragment (2.9 kb) of Bacillus cereus VKM-B771 chromosome DNA. The hemolytic product encoded by the cloned DNA fragment possessed all the properties of hemolysin II known to date: it was not inhibited by cholesterol, exhibited the Arrhenius effect, and had a relatively long (in comparison with cereolysin) lag period in erythrocyte lysis.

[Nucleotide sequence of phospholipase C and sphingomyelinase genes from Bacillus cereus BKM-B164].

A BamHI-EcoRV DNA fragment, containing tandemly arranged genes for the Bacillus cereus VKM-B164 phosphatidylcholine-specific phospholipase C and sphingomyelinase, has been cloned and sequenced in pUC19 plasmid (EMBL accession No X64141). The obtained gene sequences have been compared with those the B. cereus strains SE-1, IAM-1208 and GP-4 reported previously.

[Disorders of the immune status in patients with acute diffuse peritonitis].

Analysis of various parameters of the immune system and non-specific resistance in patients with acute generalized peritonitis (AGP) has indicated that in AGP there is immunodeficiency involving all the links of immune defense and non-specific resistance. The T-cell immunity, primarily T helpers, is afflicted in AGP to the greatest extent. A magnitude of decreases in T helper counts may be used as a prognostic indicator in AGP.

[Detoxication therapy in diffuse suppurative peritonitis].

Results of treatment of 284 patients operated upon for diffuse peritonitis are presented. The assessment is given of the significance of detoxication methods--forced fractionated peritoneal dialysis and washing of the abdominal cavity in treatment of diffuse peritonitis and their influence upon the outcome of the disease.

[Structure of the Pseudomonas aeruginosa recA gene].

The nucleotide sequence of the 1206 bp fragment of Pseudomonas aeruginosa DNA coding for the recA gene has been determined. This structure was shown to contain an open reading frame corresponding to a protein with m.w.

[Cloning of Shigella flexneri 2a genes coding for restriction- modification system Sfl2aI].

The Sfl2aI system of restriction-modification (RM) was revealed in the cells of Shigella flexneri encoded by pKMR114 plasmid belonging to the IncN incompatibility group. The genes for Sfl2aI RM system were cloned. The system was ascribed to the enzymes of the EcoRII specificity, as shown by in vivo and in vitro experiments.

[Cloning and regulation of gene expression of EcoRV restriction- modification system].

A number of recombinant plasmids, containing EcoRV restriction-modification genes have been constructed. Individual genes of this system were introduced into plasmids of various incompatibility groups. Promoter regions of genes encoding methylase and restrictase have been cloned and studied.

[Perforation of the small intestine and colon by foreign bodies].

An analysis of diagnostics and treatment of perforations of the small intestine and colon with foreign bodies in 10 patients is presented. Laparoscopy is considered to be of great diagnostic value.