Publications by authors named "Kuwata G"
Background: Compared to two-dimensional cultures, three-dimensional (3D) cultures have many advantages in cancer studies. Nevertheless, their implementation is unsatisfactory. This study aimed to develop an anchorage-dependent 3D culture model for colorectal cancer research.
View Article and Find Full Text PDFBackground/aim: The expression of corticotropin-releasing hormone (CRH)-related peptides involved in stress response in colorectal cancer has been reported. We examined the involvement of CRH-related peptides in cellular stress caused by anticancer drugs in colorectal cancer.
Materials And Methods: Changes in the expression levels of CRH-related peptides and their receptors in HCT116, DLD-1, and SW480 cell lines after fluorouracil (5-FU) loading were evaluated.
Article Synopsis
- Self-expandable metallic stents (SEMSs) are utilized to treat malignant colorectal obstructions, such as those caused by advanced gastric cancer.
- A case was reported where a patient experienced a fracture of an uncovered colonic SEMS 494 days post-insertion, highlighting a rare complication.
- As more patients receive long-term SEMS for palliative care, the occurrence of unique complications like stent fractures is likely to rise.
Article Synopsis
- - Evidence suggests a strong link between gut microbiota and acute graft-versus-host disease (aGVHD) in stem cell transplantation (SCT), leading to interest in fecal microbiota transplantation (FMT) as a treatment option.
- - A pilot study involving 4 SCT patients with steroid-resistant or steroid-dependent aGVHD showed no severe adverse effects from FMT, with positive responses in all participants (3 complete, 1 partial).
- - The study indicates that FMT can be safely implemented in these patients and may provide a promising new treatment option for aGVHD, highlighting changes in gut microbiota and the increase of regulatory T cells during recovery.
Article Synopsis
- IgG4-related disease is a newly identified condition that involves significant inflammation and fibrosis due to the presence of IgG4-positive plasma cells, affecting various organs.
- A 64-year-old man was found to have a large mass in his abdomen that was initially thought to be a tumor, leading to surgery and subsequent diagnosis of IgG4-related sclerosing mesenteritis.
- Following surgery, another mass developed near the ureter, which responded positively to steroid treatment, although it couldn't be definitively diagnosed as IgG4-related retroperitoneal fibrosis.
Article Synopsis
- IgG4-related disease (IgG4-RD) is a condition that can impact multiple organs, with a study analyzing clinical data from 132 patients revealing various underlying pathologies.
- Almost half of the patients had multiple organ involvement, showing a significant increase in serum IgG4 levels in those with more lesions.
- Specific correlations between organs were noted, with particular IgG4-RDs frequently associated with each other, although many lesions were often diagnosed at the same time.
[A case of Lynch syndrome with advanced jejunal cancer].
Nihon Shokakibyo Gakkai Zasshi
November 2014
A woman in her 80s had two episodes of ileus, which led to the diagnosis of advanced jejunal cancer. She was diagnosed with Lynch syndrome when she was in her 60s, for which she underwent annual follow-up with computed tomography for 8 years. Unfortunately, she died from the recurrence of jejunal cancer and liver metastases.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to investigate the characteristics of irregular narrowing in the main pancreatic duct (MPD) observed in patients with autoimmune pancreatitis (AIP) using endoscopic retrograde cholangiopancreatography (ERCP).
- A total of 40 AIP patients were examined, revealing that 65% showed the MPD opening sign and 78% demonstrated the distal common bile duct (CBD) sign, suggesting potential markers for diagnosing AIP.
- Histological analysis indicated lymphoplasmacytic infiltration in biopsy specimens from the major papilla, further supporting the diagnosis of AIP in cases with abnormal ERCP findings.
Article Synopsis
- - This study aimed to assess the appearance of major and minor duodenal papillae in patients suffering from autoimmune pancreatitis (AIP).
- - It involved examining endoscopic features in 72 patients, with abnormal characteristics found in 44% of major papillae and 38% of minor papillae, including swelling and redness.
- - After steroid therapy, noticeable improvements were observed in the swelling of the major and minor papillae in a subset of patients, highlighting the impact of treatment.
Article Synopsis
- * Among the AIP patients assessed, 36% had a history of allergic diseases, with conditions like allergic rhinitis and asthma being prevalent; also, elevated serum IgE was found in 60% of those tested.
- * The results indicate a potential link between allergic mechanisms and AIP, as 87% of patients evaluated showed positive allergen-specific IgE antibody tests.
Article Synopsis
- * Researchers reviewed data from 85 AIP patients and performed immunostaining on biopsy samples from 46 patients (14 with AIP and 32 with UC).
- * Results showed that while UC can occur with AIP, it is not solely a sign of IgG4-related disease; however, higher levels of IgG4-positive plasma cells in UC patients were linked to more severe disease activity.
Mixed acinar-endocrine carcinoma of the pancreas treated with S-1.
Clin J Gastroenterol
December 2013
Article Synopsis
- A 63-year-old man was diagnosed with acinar cell carcinoma of the pancreas, presenting with a large mass and multiple liver metastases.
- He underwent S-1 chemotherapy, which initially showed partial effectiveness, as indicated by decreased serum levels of CA19.9 and NSE.
- However, after 10 months, the tumor progressed, leading to liver failure and death 18 months later, with autopsy revealing a mixed acinar-endocrine carcinoma, suggesting the chemotherapy was more effective against the acinar component than the neuroendocrine differentiation.
Article Synopsis
- Autoimmune pancreatitis (AIP) lacks a specific diagnostic serological marker and is diagnosed using unique features from four different diagnostic criteria sets.
- A study compared these criteria among 85 AIP patients, finding that diagnosis rates were high: 87% with Japanese 2006, and 95% for Asian, ICDC, and Japanese 2011 criteria.
- The ICDC effectively differentiates between types 1 and 2 AIP with high sensitivity, but its complexity suggests that diagnostic criteria may need to be simplified and adapted for use in various countries.
Immunoglobulin G4-related gastrointestinal diseases, are they immunoglobulin G4-related diseases?
World J Gastroenterol
September 2013
Article Synopsis
- IgG4-related disease (RD) is characterized by organ enlargement and nodular lesions with lymphocyte and IgG4-positive plasma cell infiltration, along with fibrosis, affecting various organs.
- In autoimmune pancreatitis, IgG4-positive plasma cells are present in the gastrointestinal tract, but lack key features of IgG4-RD like mass formations or dense fibrosis, making diagnosis challenging.
- Literature indicates two types of IgG4-related gastrointestinal disease: one involves thickened walls with fibrosis and plasma cell infiltration, while the other presents as pseudotumors; accurate diagnosis is crucial to exclude malignancies, and these lesions may respond well to steroid treatment.
Article Synopsis
- * Researchers measured serum IgG4 levels and examined tissue samples from 54 IPMN patients, finding elevated IgG4 levels in only 4% of them and significant IgG4-positive plasma cell infiltration in 17% of the cases.
- * The findings suggest that while IgG4-positive plasma cell infiltration can occur around IPMNs, it is rare and does not imply a strong link to AIP type 1
Article Synopsis
- The study aimed to understand the clinical characteristics of IgG4-related retroperitoneal fibrosis (RF) through specific diagnostic criteria involving serum IgG4 levels and tissue infiltration.* -
- Ten patients were analyzed, revealing a mean age of 70.1 years, with a predominance of associated diseases over initial symptoms related to RF, which included issues like back pain and lower extremity edema.* -
- Histological confirmation of the disease was achieved in most cases, and seven patients treated with steroids showed positive responses, highlighting the importance of recognizing the condition for accurate diagnosis and management.*
Article Synopsis
- * Results showed that VD type patients experienced more obstructive jaundice (87%) compared to D type patients (0%), while abdominal pain was more common in D type (24%) than in VD type (2%).
- * The findings suggest a new condition called "autoimmune dorsal pancreatitis," characterized by involvement only of the dorsal pancreas, with less frequent complications like obstructive jaundice and lower bile duct stenosis.
Article Synopsis
- The study aims to differentiate between immunoglobulin G (IgG) 4-related sclerosing cholangitis (IgG4-SC) and hilar cholangiocarcinoma (CC) due to the lack of previous research on the topic.
- A comparison of clinical, serological, imaging, and histological features revealed that patients with CC often present with more severe jaundice and higher serum levels of specific markers, while those with IgG4-SC show elevated IgG and IgG4 levels, along with pancreas enlargement.
- The findings suggest that an integrated diagnostic approach considering all these factors is crucial for accurately distinguishing between IgG4-SC and hilar CC.
Article Synopsis
- The study investigates the characteristics of pancreatic cancer patients who experience acute pancreatitis compared to other pancreatic cancer patients without pancreatitis and patients with acute pancreatitis without cancer.
- It involved a comparative analysis of 18 patients with both pancreatic cancer and acute pancreatitis, 300 patients with pancreatic cancer but no pancreatitis, and 141 patients with acute pancreatitis only.
- The findings suggest that in cases of acute pancreatitis, especially when the cause isn't clear, doctors should evaluate for potential pancreatic cancer, even if the pancreatitis is mild.
Purpose: N (1),N (12)-Diacetylspermine (DiAcSpm) is a tumor marker featured by increase in the urine of patients with cancers, including early colorectal cancer, but where and how DiAcSpm is made remains unclear. We aimed to clarify whether colorectal cancer tissues produce increased amounts of DiAcSpm, and if they do, to examine whether tissue DiAcSpm level may serve as a criterion of tissue malignancy.
Methods: Tissue samples were obtained from 140 patients (13 low-grade intraepithelial neoplasia, 98 high-grade intraepithelial neoplasia and 29 colorectal cancer) treated for colorectal cancer and intraepithelial neoplasia at Tokyo Metropolitan Komagome Hospital between November 2007 and April 2011.
Background/aims: The objective of this study was to compare the clinical characteristics of patients with autoimmune pancreatitis (AIP) with or without Mikulicz's disease (MD) and with MD alone.
Methods: We investigated the clinical findings in 15 AIP patients with MD (group A+M), 49 AIP only patients (group A), and 14 MD only patients (group M).
Results: The male-female ratio was significantly higher in group A+M (73%, p<0.
Purpose: This study was aimed to investigate etiology and clinical profiles of recurrent acute pancreatitis (RAP), particularly from the morphology of the pancreaticobiliary duct system.
Material And Methods: Pancreaticobiliary morphology was examined in 230 of 381 patients with acute pancreatitis (AP) using endoscopic retrograde cholangiopancreatography. We analyzed factors associated with RAP including the pancreaticobiliary duct system.
Article Synopsis
- Autoimmune pancreatitis (AIP) often leads to diabetes mellitus (DM), and this study aimed to assess how steroid therapy affects DM in AIP patients.
- In a study of 69 patients with AIP, 46% had DM, and after three months of steroid treatment, about 54% of these patients showed improvement in their glucose levels.
- The findings suggest that around 60% of DM cases related to AIP respond positively to steroids over time, indicating that significant DM in these patients may warrant steroid therapy. *