[Degradation of the EDTA and EDTA complexes with metals by immobilized cells of Chelativorans oligotrophicus LPM-4 bacteria].

Enzymatic oxidative degradation of EDTA and EDTA complexes with metals has been investigated using immobilized cells of Chelativorans oligotrophicus LPM-4. A polarographic method, which makes it possible to register oxygen consumption by cells, has been used. For the first time, it has been indicated that the Cd-EDTA and Ni-EDTA complexes undergo degradation by the bacteria under study.

[Degradation of p-toluenesulfonate by immobilized Comamonas testosteroni BS1310 (pBS1010) cells].

Parameters of degradation of p-toluenesulfonate (TS) by free and agar-embedded Comamonas testosteroni BS1310 (pBS1010) cells were determined. The maximum rate of TS degradation was 25% lower in by immobilized than free cells, equaling 11 nmol x min(-1) x mg(-1) cells. Degradation of TS by both free and immobilized cells was associated with molecular oxygen consumption (molar ratio, 1 : 2).

[Degradation of 2,4-dinitrophenol by free and immobilized cells of Rhodococcus erythropolis HL PM-1].

Degradation of 2,4-dinitrophenol (2,4-DNP) by the cells of Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity.

[Biosensor of the reactor type based on Rhodococcus erythropolis HL TM-1 cells for determining 2,4-dinitrophenol].

A model of a reactor-type biosensor based on the Rhodococcus erythropolis HL PM-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-DNP). The effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. In case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-DNP detection was 20 microM and the time of single analysis, the biosensor recovery included, was 30-50 min.

[Development of biosensors for phenol determination from bacteria found in petroleum fields of West Siberia].

Nine Gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation.