Two novel HLA-DRB1 alleles, HLA-DRB1*04:333 and -DRB1*15:01:48, identified by sequencing in Russian individuals.

Characterization of the novel HLA-DRB1*04:333 and HLA-DRB1*15:01:48 alleles in two Russian individuals from Irkutsk.

The HLA-B*58:01:42 allele identified in a volunteer bone marrow donor.

HLA-B*58:01:42 differs from HLA-B*58:01:01:01 in codon 303 in exon 5.

Two novel HLA-C alleles, HLA-C*04:450 and HLA-C*15:02:51, detected in individuals from Russia.

Two novel HLA-C alleles, HLA-C*04:450, and HLA-C*15:02:51, were identified.

Detection of an HLA-DRB1*07 variant, HLA-DRB1*07:130, in a Russian Kalmyk individual.

One nucleotide replacement in codon 177 of HLA-DRB1*07:01:01:01 results in a novel allele, HLA-DRB1*07:130.

A novel HLA-B allele, HLA-B*50:04:02, detected in a Russian hematopoietic stem cell donor.

HLA-B*50:04:02 differs from HLA-B*50:04:01 by two silent mutations.

Characterization of the novel HLA-A*03:01:102 allele by next-generation sequencing.

HLA-A*03:01:102 differs from HLA-A*03:01:01:01 by one nucleotide substitution at position 72 in exon 1.

Two novel HLA alleles, HLA-DRB1*12:90 and HLA-DQB1*03:458, identified by next-generation sequencing.

Two novel HLA alleles DRB1*12:90 and DQB1*03:458 have non-synonymous mutations in exon 3.

The novel HLA-B allele, HLA-B*55:01:27.

HLA-B*55:01:27 differs from HLA-B*55:01:01:01 by a mutation at nucleotide 873.

Buryats from Republic of Buryatia and Irkutsk Region, Russia.

NGS typing reveals six novel alleles among 192 class I and class II alleles.

Identification of three novel HLA alleles: HLA-A*68:01:58, -B*27:05:52 and -DRB1*14:04:09.

Three novel alleles were identified during routine next generation sequencing.

Characterization of two new human leukocyte antigen alleles: HLA-A*02:982 and HLA-C*04:441.

Two new alleles were identified during routine next generation sequencing.

Three novel HLA alleles detected in individuals from Russia: HLA-A*26:209, -DRB1*03:01:33, and -DQB1*03:447.

Three new alleles were identified during routine next generation sequencing.

Recognition of a novel HLA-B*13 allele, HLA-B*13:153, in a Russian individual.

One nucleotide replacement in codon 144 of HLA-B*13:45 results in a novel allele, HLA-B*13:153.

A novel allele, HLA-C*15:227, identified when typing COVID-19 patients.

HLA-C*15:227 differs from HLA-C*15:02:01:01 by a single nonsynonymous change (368A → G Tyrosine 99 to Cysteine).

The novel HLA-DQB1*05:02:24 allele, identified in a Russian bone marrow donor.

The new allele HLA-DQB1*05:02:24 showed one synonymous nucleotide difference with HLA-DQB1*05:02:01:01 in codon 140.

Two new HLA alleles, HLA-B*15:583 and DRB1*11:279, detected in individuals from the Irkutsk region.

The new HLA-B*15:583 and DRB1*11:279 alleles identified in Buryat and Russian individuals, respectively.

Characterization of two novel HLA alleles, HLA-A*25:71 and HLA-C*07:899 in Russian individuals.

Two novel HLA alleles were detected using next-generation sequencing.

Kalmyks from Republic of Kalmykia, Russia.

NGS typing reveals 2 novel alleles amongst 206 class I and class II alleles.

The novel HLA-A allele, HLA-A*01:354, identified in a Buryat individual.

HLA-A*01:354 differs from HLA-A*01:01:01:01 by a single nonsynonymous change (124G->A, glycine 18 to arginine).

Two new HLA alleles, HLA-B*18:200 and HLA-C*04:435 detected in Russian donors.

Two novel alleles, HLA-B*18:200 and HLA-C*04:435, are characterized.

The novel HLA-A allele, HLA-A*01:353.

HLA-A*01:353 differs from A*01:01:01:01 by a mutation at nucleotide 728.

The novel HLA-B*57:135 allele was identified during high-resolution HLA typing.

HLA-B*57:135 differs from HLA-B*57:01:01:01 by one nucleotide exchange at position 692 (G->A).

[Expirience introduction of quantitative analysis of chimerism after allogenic stem cell transplantation by real-time PCR with InDel polymorphism.].

Using data obtained from domestic and foreign sources, we formed a set of primers and fluorogenic probes for analyzing twentysix specific sequence polymorphisms and one reference gene. In the course of evaluating the effectiveness of real-time PCR, using the example of one of the markers (S01a), we obtained the optimal amount of DNA per reaction (70 ng), providing a resolution of at least 0.1% of the method with the ability to estimate linear chimerism.

[Features of distribution HLAalleles and haplotypes in Kalmyks.].

Conducted high-resolution HLA-typing loci HLA-A, -B, -C, -DRB1 and -DQB1 by massively parallel sequencing of 150 potential donors of hematopoietic stem cells from the Republic of Kalmykia. In the studied population, four new alleles identified that not previously registered by the International Committee on the Nomenclature of Factors of the HLA-system of WHO. During the HLA-typing identified: 29 alleles at the HLA-A locus, 44 - at the HLA-B locus, 26 - at the HLA-C locus, 15 - at the DQB1 locus, 37 - at the HLA-DRB1 locus.