Increased dementia risk in patients with Parkinson's disease attributed to metabolic syndrome.

Introduction: Metabolic syndrome (MetS) manifests resembling pathophysiological mechanisms with Parkinson's disease (PD). Current research on the overall population has emphasized MetS as a freestanding risk factor for cognition. This research aims to explore the impact of MetS on cognition in Parkinson's patients.

The role of ceranib-2 and its nanoform on the decrease of telomerase levels in human non-small cell cancer.

Background: Ceranib-2, an acid ceramidase (AC) inhibitor, can inhibit cancer cell proliferation and tumor development. However, poor water solubility and low cellular bioavailability limit its efficacy in cancer treatment.

Methods And Results: This study aimed to investigate the cell death induced by ceranib-2 and its solid lipid nanoformulation (ceranib-2-SLN) produced by the hot homogenization technique and the synergistic relationship between ceramide and telomerase in vitro and in silico.

Exogenous Ceramide Treatment Induce Death and Cytotoxicity in Glioma Cells.

Aim: To evaluate the cytotoxic and proapoptotic effects of C6 ceramide on the C6 rat glioma cell line.

Material And Methods: The C6 rat glioma cell line was evaluated. Using a confocal microscope and the appropriate software, the cytotoxic effects of C6 ceramide were identified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) colorimetric experiments.

Presence of candida in the dental plaque and saliva of patients with severe early childhood caries and early childhood caries:a pilot study.

Purpose: The aim of this study is to evaluate the presence of candida, which is one of the etiological factors contributing to early childhood caries (ECC) and severe early childhood caries (S-ECC), in the dental plaque and saliva of children aged 6 years and younger.

Materials And Methods: Our study involved 60 participants who met the inclusion criteria. Based on clinical examinations, we divided them into three groups, each consisting of 20 children: S-ECC, ECC, and caries-free groups.

Proapoptotic effects of ceranib-2 in combination with radiation therapy on human breast cancer cells.

Objective: Strategies for cancer therapy involve radiation therapy (RT), which accounts for about 40% of all cancer treatment types. As to current chemotherapeutics, cancer cells also develop resistance that remains a clinical problem, such as disease recurrence. Recent studies focused on understanding the molecular mechanisms of radiation-induced cell death.

Investigation of proapoptotic and cytotoxic effects of 2-aminobenzothiazole on human laryngeal carcinoma cells.

Objective: In the present study, we investigated the effects of 2-aminobenzothiazole application on human laryngeal carcinoma cells.

Materials And Methods: Human larynx epidermoid carcinoma (HEp-2) (ATCC® CCL-23™) cells were purchased from American Type Culture Collection (ATCC, USA). Human larynx epidermoid carcinoma HEp-2 cells were cultured in complete Dulbecco's Modified Eagle's Medium (DMEM) supplemented with fetal bovine serum (FBS) (10%) and penicillin/streptomycin (1%) in a CO2 (5%) incubator under standard cell culture conditions.

The emergence of Klebsiella pneumoniae sequence type 395 non-susceptible to carbapenems and colistin from Turkey.

Purpose: OXA-48 producing Klebsiella pneumoniae is an emerging threat and outbreaks due to specific sequence types have been commonly reported. Here, we report an outbreak due to multidrug-resistant ST395 K. pneumoniae ST395.

Effects of ceramide C2 application on human laryngeal carcinoma cells: a cell culture study.

Objective: In the present study, we investigated the effects of Ceramide C2 application on human laryngeal carcinoma cells.

Materials And Methods: Human larynx epidermoid carcinoma HEp-2 (ATCC® CCL-23™) cells were purchased from the American Type Culture Collection (ATCC, USA). Human larynx epidermoid carcinoma HEp-2 cells were cultured in complete Dulbecco's Modified Eagle's Medium (DMEM) supplemented with fetal bovine serum (FBS) (10%) and penicillin/streptomycin (1%) in a CO2 (5%) incubator under standard cell culture conditions.

Effect of different litter materials on growth performance, the gait score and footpad dermatitis, carcass parameters, meat quality, and microbial load of litter in broiler chickens.

In the current study, the microbial load of litter materials, growth performance, the gait score and footpad dermatitis, carcass characteristics, and meat quality of broilers reared on different litter materials were investigated. After hatching and sex determination, the chicks were allocated to 3 different experimental groups with each having 8 replications. The chicks were reared on thick sawdust, fine sawdust, or rice hull litter materials.

Evaluation of spiramycin for topical applications: a cell culture study.

Objective: Through a cell culture test, we analyzed the cytotoxic effects of topical spiramycin on NIH/3T3 fibroblast cells.

Materials And Methods: Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin was used for the growth of NIH/3T3 fibroblast cells in a 5% CO2 incubator. Spiramycin's cytotoxicity was measured using the MTT assay.

Bromelain: a candidate to enhance wound healing after endonasal surgeries.

Objective: In the present study, we investigated the topical bromelain's cytotoxic effects on mouse fibroblast NIH/3T3 cells via cell culture study.

Materials And Methods: In this cell culture study, Dulbecco's Modified Eagle Medium (DMEM) with fetal bovine serum (FBS, 10%) and penicillin/streptomycin (1%) was used as a cell growth medium for NIH/3T3 mouse fibroblast cells. MTT test was performed in 96-well plates seeded with NIH/3T3 cells 5x103/well and under standard cell culture conditions.

Efficacy and toxicity of anise oil as a potential topical wound healer: a cell culture study.

Objective: We studied the cytotoxic effects of topical anise oil on NIH/3T3 fibroblast cells using a cell culture assay.

Materials And Methods: NIH/3T3 fibroblast cells were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (10%) and penicillin/streptomycin under standard cell culture conditions in a humidified incubator containing 5% carbon dioxide. For the MTT cytotoxicity experiment, NIH/3T3 cells were plated in triplicate at a concentration of 3x103 per well in 96-well plates and incubated for 24 hours.

Efficacy and toxicity of Anatolian propolis on healthy nasal epithelial cells.

Objective: In our study we aimed to evaluate the effects of applying propolis topically to epithelial cells of the nasal cells, to discover whether this causes any toxic effect upon the cells.

Materials And Methods: Samples of healthy human primary nasal epithelium harvested during septoplasty from volunteers were incubated in cell culture. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays may be utilized when assessing cellular damage (toxicity), as evidenced by DNA fragmentation, nuclear condensation, alteration in the outer plasma membrane and cytoskeletal alteration.

Evaluation of ciprofloxacin used as an intranasal antibiotic.

Objective: The objective of this research was to examine the effects of topical ciprofloxacin on cultured nasal epithelial cells of human origin.

Materials And Methods: Human nasal epithelial cells were collected from patients who voluntarily donated tissue left over following septorhinoplasty. The samples were from individuals without any indication of rhinosinusitis.

An evaluation of ketoprofen as an intranasal anti-inflammatory agent.

Objective: The objective of the study was to evaluate the effect of ketoprofen when locally applied to tissue-cultured nasal epithelium.

Materials And Methods: Healthy primary nasal epithelial cells were grown in a tissue culture medium. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to evaluate cytotoxicity.

A safety investigation into topical effects of naproxen sodium on nasal epithelial cells and potential toxicity in local application.

Objectives: We examined how topically-applied naproxen sodium affects human nasal epitheliocytes in culture.

Methods: Samples of healthy human primary nasal epithelium (NE) harvested during septoplasty from volunteers without rhinosinusitis were incubated in cell culture. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays may be utilised when assessing cellular damage (toxicity), as evidenced by DNA fragmentation, nuclear condensation, alteration in the outer plasma membrane and cytoskeletal alteration.

15-year evaluation of changes in the HBsAg positivity rate in pregnant women in Turkey: the prominent effect of national vaccination.

Background: The detection of hepatitis B surface antigen positivity in pregnant women before delivery is crucial to preventing mother-to-child transmission of hepatitis B virus.

Aims: This study aimed to evaluate the status and rate of testing for hepatitis B surface antigen, rate of hepatitis B surface antigen positivity, hepatitis B surface antigen positivity distribution rate by age, and changes in hepatitis B surface antigen positivity rate in pregnant women over the study period.

Methods: We conducted a multicentre, cross-sectional, descriptive study covering the period January 2005 to June 2019 for 2 145 668 pregnant women from 27 provinces in all 7 regions of Turkey, collected using Microsoft Excel before statistical analysis.

Post-mating diclofenac vs. carprofen treatment on serum progesterone levels and reproductive outcomes in Hungarian-Merino ewes during the non-breeding season.

The maternal recognition process is crucial for the establishment of healthy pregnancy. In this process, anti-luteolytic applications are one of the main reproductive strategies to manage the embryonic losses and maximize reproductive profitability in farm animals. The aim of this study was to investigate the efficacy of post-mating NSAID treatments on reproductive parameters (pregnancy rate, lambing rate, multiple birth rate, litter size) and serum progesterone levels in ewes stimulated with progesterone non-breeding season.

Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens.

The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers. For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each.

The effects of thymoquinone and quercetin on the toxicity of acrylamide in rat glioma cells.

Acrylamide is a neurotoxic agent forming in foods. Thymoquinone and quercetin are plant-derived antioxidants in various foods with known benefits. C6 cells are glioblastoma cells.

Prevalence of HBsAg seropositivity during pregnancy and evaluation of vaccination programs: A multicenter study in Turkey.

Objective: Hepatitis B virus (HBV) infection remains a global public health problem. Among its modes of transmission, vertical transmission from mother to child during pregnancy is exceedingly important. This study investigated seropositivity for hepatitis B surface antigen (HBsAg) among pregnant women aged 16-49 years and their pregnancy outcomes in several health institutions (university and state hospitals, family health centers) from seven cities in Turkey.

Cytotoxic Impact of N-Oleoylethanolamine on Bone Cancer Cells.

Background: Cancer is a complex disease that derives from the uncontrolled proliferation of cells. Bone cancer is a type of prevalent cancer that occurs both in young and adults. Bone cancer is most common in the long bones of the pelvis, arms and legs.