Evaluation of the Efficacy of Human Dental Pulp Stem Cell Transplantation in Sprague-Dawley Rats with Sensorial Neural Hearing Loss.

Objectives:  The purpose of the present study was to evaluate the efficacy of spiral ganglion neuron (SGN) regeneration after dental pulp stem cell (DPSC) transplantation in a rat sensorineural hearing loss (HL) model.

Materials And Methods:  Sham or experimental HL was induced in adult Sprague-Dawley rats by cochlear round window surgery. An HL rat model was established with a single 10 mM ouabain intratympanic injection.

Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells.

Objectives: Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons.

Neuronal differentiation of dental pulp stem cells from human permanent and deciduous teeth following coculture with rat auditory brainstem slices.

Sensorineural hearing loss is a common disability found worldwide which is associated with a degeneration of spiral ganglion neurons (SGN). It is a challenge to restore SGN due to the permanent degeneration and viability of SGN is requisite for patients to receive an advantage from hearing aid devices. Human dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) are self-renewing stem cells that originate from the neural crest during development.

Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells.

Objective: Stem cells from pulp tissue are a promising cell-based therapy for neurodegenerative patients based on their origin in the neural crest. The aim of this study was to differentiate and evaluate the ability of human dental pulp stem cells from permanent teeth (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) to differentiate into spiral ganglion neurons.

Design: After isolation and characterization of mesenchymal stem cell properties, DPSC and SHED were treated with the neurotrophins brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and glial cell-derived neurotrophic factor (GDNF).

EFFECTIVENESS OF AN ORAL HYGIENE EDUCATION PROGRAM COMBINED WITH FLUORIDE MOUTHRINSE AMONG VISUALLY IMPAIRED STUDENTS IN BANGKOK, THAILAND.

The objective of this study was to assess the effectiveness of oral hygiene education kits (OHE kits) and 0.05% sodium fluoride mouth rinse among visually impaired students (VIS) in Bangkok, Thailand. Seventy-five VIS aged 10-12 years were included in the study and examined for plaque index (PI), gingival index (GI) and mutans streptococci (MS) salivary levels at baseline and after intervention.