[Manganese content in chlorophyll-protein complexes of higher plants].

The incorporation of manganese in pigment-protein lamellae complexes was studied. Manganese content was estimated by radioisotopic technique after the precipitation by ammonium sulfate, gel filtration, and polyacrylamide gel electrophoresis of isolated complexes. None of isolated complexes showed manganese content more than 10(-10) g/mg of protein.

[Photochemical and spectral properties of photosystem-2 subchloroplast fragments, highly purified from photosystem-1 mixtures].

A rather simple method of isolation of photosystem 2 fragments, which are highly purified from Photosystem 1 admixture, has been developed on the basis of combined action of detergents and differential centrifugation. The isolated fragments are characterized by insignificant content of P700 (one molecule per 10500 molecules of chlorophyll) and by high ratio of band values at 685 and 735 nm in the low temperature emission spectrum of fluorescence (F685/F735=5.9).

[Method of isolating photochemically active pigmet-proteolipid complexes from chloroplasts].

The paper deals with the chromatography isolation method of pigment-lipoprotein complexes of photosystem I and II of pea chloroplasts on the DEAE-cellulose. The componenet composition and photochemical properties of isolated complexes are compared with the data available in literature concerning similar objects. The question about the use of pigment-lipoprotein complexes as the model systems for investigating structure organization and functional characteristics of different parts of the chloroplast photosynthetic apparatus is under discussion.