Hemorrhagic Fever With Renal Syndrome in Vladivostok City, Russia.

Hemorrhagic fever with renal syndrome (HFRS) is a public health problem in Vladivostok city, Russia. From 1997 to 2019, a study of hantaviruses in Norway rats (), a natural reservoir of Seoul virus (SEOV), and in HFRS patients was conducted. We demonstrated the presence of SEOV in the local population of Norway rats and detected SEOV in 10, Amur virus (AMRV) in 4 and Hantaan virus (HTNV) in 1 out of 15 HFRS patients.

[Dynamics of hantavirus detection in the excretory organs of Apodemus mice and its relation to epidemic manifestations of hantavirus infection].

This paper presents data on the detection of hantavirus in the excretory organs of Apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the Primorye Region. Acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. There was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity and activity of hantavirus reproduction in the murine excretory organs.

[Time course of changes in antibodies of various avidity in murine rodents in the epizootic and epidemic processes in hantavirus infection].

The paper presents the results of studying the time course of changes in antibodies of varying avidity in murine rodents. The presence of low-avidity antibodies is shown to reflect the status of acute infection in the rodent population and their active viral shedding. A relationship is shown between the detection of low-avidity antibodies in animals in spring and autumn and the rise of endemic hemorrhagic fever (EHF) morbidity.

[A new genetic variant of the PUUV virus from the Maritime Territory and its natural carrier red-grey vole Clethrionomys rufocanus].

Pulmonary tissues of red-grey voles, Clethrionomys rufocanus from Shkotovo District and Maritime Territory were investigated. rt-PCR was used to detect the hantavirus nt-222 M-segment genome and nt-403 mitochondrial DNA fragment. A new genetic variant of the PUUV virus named as "Shkotovo", that is different from other PUUV strains by 18-23%, was shown to be circulating in red-grey voles.

[Use of kinetic hemagglutination inhibition test in study of antigenic relationships of hantavirus strains circulating in foci of hemorrhagic fever with renal syndrome in Southern Far East of Russia].

The kinetics of reaction between hemagglutinating antigens and immune sera to 10 hantavirus strains (7 isolated in the Primorye territory and 3 in other regions) was studied in the cross kinetic hemagglutination inhibition (KHAI) test. An A parameter is suggested for evaluation of the similarity and differences between the studied strains. This parameter helped detect antigenic differences between Hantaan and Seoul viruses and the inter-type differences between Hantaan-like strains.

[Functional activity of hemagglutinins from Hantaan viruses strains and use of hemagglutinating antigens for serodiagnosis hemorrhagic fever with kidney syndrome].

Hemagglutinins of Hantaan virus strains isolated in Southern Far East of Russia from patients with hemorrhagic fever with the renal syndrome and from rodents, natural vectors of the virus, were studied in the cross kinetic hemagglutination inhibition test with immune sera to the studied strains. The strains differed by the functional activity index. Use of hemagglutinins of functionally active Hantaan strains in serologic studies notably improves the diagnostic efficacy of the hemagglutination test in Hantavirus infection.

[Detection of hemagglutinins in hantavirus serotypes and some of their properties].

Hemagglutinating properties of various serological types of hantavirus isolated in the Primorye area from humans with hemorrhagic fever with the renal syndrome and rodents, natural carriers of the virus, were studied. The strains were found to differ by capacity to accumulate hemagglutinin at multiplication in Vero E-6 cells. A method for elevating hemagglutinin titers by combined treatment of cultural virus-containing fluid with polyethyleneglycol-6000 and Tween-80-ether or acetone-ultrasound is suggested.

Avidin attachment to biotinylated human neutrophils induces generation of superoxide anion.

The influence of biotinylation and subsequent attachment of avidin on generation of superoxide anion by human neutrophils was studied. Biotinylation of human neutrophils with succinimide ester of biotin does not reduce superoxide generation in response to activation with phorbol myristate acetate (PMA) and formyl peptide (FMLP). Addition of avidin to biotinylated, but not native, leukocytes induces generation of superoxide anion.