Films Based on Chitosan/Konjac Glucomannan Blend Containing Resveratrol for Potential Skin Application.

Biopolymers represent a significant class of materials with potential applications in skin care due to their beneficial properties. Resveratrol is a natural substance that exhibits a range of biological activities, including the scavenging of free radicals and anti-inflammatory and anti-aging effects. In this study, chitosan/konjac glucomannan resveratrol-enriched thin films were prepared.

A phase I clinical trial of intrahepatic artery delivery of TG6002 in combination with oral 5-fluorocytosine in patients with liver-dominant metastatic colorectal cancer.

Background: Effective treatment for patients with metastatic cancer is limited, particularly for colorectal cancer patients with metastatic liver lesions (mCRC), where accessibility to numerous tumours is essential for favourable clinical outcomes. Oncolytic viruses (OVs) selectively replicate in cancer cells; however, direct targeting of inaccessible lesions is limited when using conventional intravenous or intratumoural administration routes.

Methods: We conducted a multi-centre, dose-escalation, phase I study of vaccinia virus, TG6002, via intrahepatic artery (IHA) delivery in combination with the oral pro-drug 5-fluorocytosine to fifteen mCRC patients.

Novel Methods in Acne Treatment and Skin Care: A Review.

Acne skin care aims to reduce the stratum corneum layer, normalization of sebum secretion, and mitigation of purulent lesions. Antibiotics, such as macrolides, clindamycin, and tetracyclines, are often used in acne treatment. In clinical practice, the resistance of antibiotics has become an increasing issue.

Physical Properties and Release Profiles of Chitosan Mixture Films Containing Salicin, Glycerin and Hyaluronic Acid.

Chitosan (CS) has gained considerable attention due to its distinctive properties and its broad spectrum of potential applications, spanning cosmetics, pharmaceuticals, and biomedical uses. In this study, we characterized thin films comprising chitosan mixtures containing salicin (SAL) and glycerin (GLY), both with and without hyaluronic acid (HA) as active ingredients. Characterization was achieved through release studies of SAL, infrared spectroscopy, microscopy techniques (AFM and SEM), and thermogravimetric analysis (TGA).

Chitosan-Based Films Containing Rutin for Potential Cosmetic Applications.

Chitosan is a polysaccharide with film-forming properties. Such properties are widely used for the preparation of beauty masks and wound-healing materials. In this work, chitosan-based films containing hyaluronic acid and rutin have been researched for potential cosmetic applications.

The Selection of the Best Derivatization Reagents for the Determination of Polyamines in Home-Made Wine Samples.

The procedures of putrescine, spermine, spermidine, and cadaverine derivatization using 2-chloro-1,3-dinitro-5-(trifluoromethyl)benzene, 1-fluoro-2-nitro-4-(trifluoromethyl) benzene, and 3,5-bis-(trifluoromethyl)phenyl isothiocyanate for chromatographic determination in home-made wine samples are compared in the present study. The procedures discussed were compared regarding simplicity, linearity, precision, and accuracy. The polyamines derivatives were isolated and characterized by X-ray crystallography and H, C, and F NMR spectroscopy.

Simple and Effective Derivatization of Amino Acids with 1-Fluoro-2-nitro-4-(trifluoromethyl)benzene in a Microwave Reactor for Determination of Free Amino Acids in Kombucha Beverages.

Kombucha is a fermentation product of sweetened tea with a symbiotic culture of acetic acid and yeast bacteria, consumed worldwide for its health-promoting properties. Few reports can be found about free amino acids among the health-promoting compounds found and determined in kombucha. These compounds influence the sensory properties of kombucha, and they are precursors of bioactive compounds, which have a significant role as neurotransmitters and are involved in biological functions.

Intravenous Oncolytic Vaccinia Virus Therapy Results in a Differential Immune Response between Cancer Patients.

is an engineered Wyeth-strain vaccinia oncolytic virus (OV), which has been tested extensively in clinical trials, demonstrating enhanced cytotoxic T cell infiltration into tumours following treatment. Favourable immune consequences to include the induction of an interferon (IFN) response, followed by inflammatory cytokine/chemokine secretion. This promotes tumour immune infiltration, innate and adaptive immune cell activation and T cell priming, culminating in targeted tumour cell killing, i.

Complementary and Alternative Medicine Use in Hospitalized Cancer Patients-Study from Silesia, Poland.

Background: The use of complementary and alternative medicine (CAM) is common amongst cancer patients. The aim of the study was to investigate the use of CAM, beliefs about CAM and the purpose of using it amongst Polish cancer patients.

Methods: The study included 864 cancer patients (median 63 years old), who were individually interviewed.

Application of Multi-Branch Cauer Circuits in the Analysis of Electromagnetic Transducers Used in Wireless Transfer Power Systems.

In this paper, the feasibility of applying a multi-branch equivalent model employing first- and second-order Cauer circuits for the analysis of electromagnetic transducers used in systems of wireless power transfer is discussed. A method of formulating an equivalent model (EqM) is presented, and an example is shown for a wireless power transfer system (WPTS) consisting of an air transformer with field concentrators. A method is proposed to synthesize the EqM of the considered transducer based on the time-harmonic field model, an optimization algorithm employing the evolution strategy (ES) and the equivalent Cauer circuits.

Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy.

Stable, single α-helical (SAH) domains exist in a number of unconventional myosin isoforms, as well as other proteins. These domains are formed from sequences rich in charged residues (Arg, Lys, and Glu), they can be hundreds of residues long, and in isolation they can tolerate significant changes in pH and salt concentration without loss in helicity. Here we describe methods for the preparation and purification of SAH domains and SAH domain-containing constructs, using the myosin 10 SAH domain as an example.

Lipid profile and effect of statin treatment in pooled phase II and phase III baricitinib studies.

Objectives: Lipid profiles are altered by active disease in patients with rheumatoid arthritis (RA) and may be further modified by treatment with Janus kinase inhibitors and other disease-modifying antirheumatic drugs.

Methods: Lipid data were analysed from phase II and III studies of 4 mg (n=997) and 2 mg (n=479) oral baricitinib administered once daily in patients with moderate-to-severe active RA. Lipoprotein particle size and number and GlycA were evaluated with nuclear magnetic resonance in one phase III study.

Determination of mycotoxins, alkaloids, phytochemicals, antioxidants and cytotoxicity in Asiatic ginseng (Ashwagandha, Dong quai, ).

Mycotoxins and selected hazardous alkaloids in the medicinal plants (Panax , , ) and dietary supplements were determined. Purine alkaloids were found in majority of samples; however, isoquinoline alkaloids were less abundant than indole. The predominant alkaloids appear to be caffeine (purine group), harman (indole group) and berberine (isoquinoline).

Characterization of long and stable de novo single alpha-helix domains provides novel insight into their stability.

Naturally-occurring single α-helices (SAHs), are rich in Arg (R), Glu (E) and Lys (K) residues, and stabilized by multiple salt bridges. Understanding how salt bridges promote their stability is challenging as SAHs are long and their sequences highly variable. Thus, we designed and tested simple de novo 98-residue polypeptides containing 7-residue repeats (AEEEXXX, where X is K or R) expected to promote salt-bridge formation between Glu and Lys/Arg.

Determination of phytochemicals, antioxidant activity and total phenolic content in Andrographis paniculata using chromatographic methods.

Antioxidant activity, total phenolics content and selected phytochemicals (alkaloids and andrographolides) were determined in Andrographis paniculata and in dietary supplements containing this plant. Antioxidant activity was measured by FRAP, CUPRAC and DPPH procedures and ranged from 503.36 to 6164.

Determination of toxic metals by ICP-MS in Asiatic and European medicinal plants and dietary supplements.

The potentially toxic metals content was determined in selected plants, used in Traditional Chinese Medicine (Angelica sinensis, Bacopa monnieri, Bupleurum sinensis, Curcuma longa, Cola accuminata, Emblica officinalis, Garcinia cambogia, Mucuna pruriens, Ocimum sanctum, Panax ginseng, Pueraria lobata, Salvia miltiorrhiza, Schisandra sinensis, Scutellaria baicalensis, Siraitia grosvenorii, Terminalia arjuna and Terminalia chebula), and some European herbs (Echinacea purpurea, Hypericum perforatum, Vitis vinifera). Samples were mineralized in a closed microwave system using HNO3 and the concentrations of Cd, Pb, Al, As, Ba, Ni and Sb were determined by ICP-MS method. Some relevant aspects of potential toxicity of metallic elements and their compounds were also discussed.

Complete genome sequence of Gluconacetobacter xylinus E25 strain--valuable and effective producer of bacterial nanocellulose.

This study reports the release of complete genome sequence of the producer of bacterial nanocellulose (BNC) - Gluconacetobacter xylinus E25, a vinegar-isolated strain. Preliminary sequence analysis revealed complexity of the genome structure and familiarized genetic basis of productive properties of E25 strain. The genome consists of one chromosome and five plasmids.

The oxidative degradation of dibenzoazepine derivatives by cerium(IV) complexes in acidic sulfate media.

The kinetics of the oxidation of imipramine and desipramine using cerium(IV) complexes were studied in the presence of a large excess of azepine derivative (TCA) in acidic sulfate media using UV-Vis spectroscopy. The reaction proceeds via dibenzoazepine radical formation, identified by EPR measurements. The kinetics of the first degradation step were studied independently of the further slower degradation reactions.

Photochemical behaviour of hydrolysed keratin.

An investigation into the influence of UV irradiation on keratin hydrolysates was carried out using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. It was found that the absorption of keratin hydrolysates in solution increased during irradiation of the sample, most notably between 250-280 and 320-410 nm. The increase in absorbance in the region 320-410 was because of the new photoproducts formed during UV irradiation of keratin hydrolysates.

Mitochondrial dysfunction in Parkinson's disease.

Parkinson's disease (PD) is a progressive, neurodegenerative condition that has increasingly been linked with mitochondrial dysfunction and inhibition of the electron transport chain. This inhibition leads to the generation of reactive oxygen species and depletion of cellular energy levels, which can consequently cause cellular damage and death mediated by oxidative stress and excitotoxicity. A number of genes that have been shown to have links with inherited forms of PD encode mitochondrial proteins or proteins implicated in mitochondrial dysfunction, supporting the central involvement of mitochondria in PD.

Indirect determination of neomycin trisulphate as sulphate by column coupling capillary isotachophoresis.

Anionic capillary isotachophoresis (cITP) with conductometric detection was used for indirect determination of neomycin trisulphate (NMS) as sulphate anion. The following electroylets were tested: leading (LE)--8 mM HCl adjusted with beta-alanine to pH = 3.55, 3 mM bis-tris-propane and 0.

Electrochemical determination of oxytetracycline in veterinary drugs.

Conductometric, potentiometric and cyclic voltammetric (CV) titration methods are proposed for determination of oxytetracycline (OTC), commonly used in veterinary. The electrochemical titration of OTC hydrochloride with NH(4)Mo(7)O(24), NaVO(3), NaOH, AgNO(3) and FeCl(3) as titrants are reported. The proposed methods were found to be highly precise, having a relative standard deviation (R.

Spectrofluorometric determination of 2- and 10-disubstituted phenothiazines.

Spectrofluorometric method for determination of promethazine hydrochloride, thioridazine hydrochloride and perazine dimaleate in the pure form and in drugs is described. Fluorescence excitation spectra of series of aqueous solutions were measured. The fluorescence signal was found to be a linear function of the promethazine hydrochloride (PTM) concentration in the range: 0.

Quantitative determination of selected tricyclic biological active compounds by using capillary electrophoresis.

Five tricyclic antidepressants: imipramine hydrochloride, noxiptilin hydrochloride, hydroxyzine dihydrochloride, diethiazine hydrochloride and amitryptyline hydrochloride were determined by the capillary electrophoresis method. The determinations were performed by using a fused sillica capillary (60 cm x 50 microns i.d.