[Lymphocytes enzymatic status and peripheral blood neutrophils oxygen-dependent metabolism in patients with renal cancer].

The immune system, one of the most important homeostatic organism systems, is actively involved in the protection against malignant tumors. The earliest sighs of immune homeostasis disorders should be invetigated at the cellular level, because of cell functional manifestations depend on the state of intracellular metabolic reactions. The study of lymphocyte NAD(P)-dependent dehydrogenases activity and peripheral blood neutrophils oxygen-dependent metabolism in patients with renal cellular carcinoma (RCC) showed a decrease in the intensity of ribose-5-phosphate and NADH-dependent synthetic processes, inhibition of terminal reactions of glycolysis.

[The immunological characteristics and enzymatic activity of lymphocytes from the peripheral blood of the children presenting with pharyngeal tonsillar hypertrophy].

The objective of the present work was to study the immunological characteristics and activity of NAD(P)-dependent dehydrogenases in peripheral blood lymphocytes in the young children presenting with pharyngeal tonsil hypertrophy (PTH). A total of 57 children at the age from 1 to 3 years with PTH were available for the examination. The control group was comprised of 35 age-matched practically healthy children.

[Changes of the Immunophenotypic Spectrum and the Enzymatic Profile of Peripheral Blood Lymphocytes in Infants with Hypertrophy of the Pharyngeal Tonsil].

Objective: to study immunophenotype and NAD- and NAD(P)-dependent dehydrogenase of blood lymphocytes activity indicators in children with hypertrophy of the pharyngeal tonsils (HPT).

Methods: 57 children aged 1-3 years with HPT were examined. The focus group included 35 healthy children of the similar age.

[Changes in functional activity of neutrophils in peripheral blood of patients with renal cell cancer in the course of the disease].

Objective: Research purpose was to study phagocytic activity and a oxygen depended metabolism of blood neutrophils (NF) at patients with a renal cell carcinoma in dynamics - before operation and after surgical treatment. METHODs; Patients with a locally invasive renal cell carcinoma (T3N0M0) were put under observation before surgical treatment (n =84) and in 10 days after a radical nephrectomy (n =54) at the age of 45-55 years. The 56 conditionally healthy donors combine into the control group.

[Immunologic indexes, enzyme status of lymphocytes and functional activity of blood neutrophils in children with infectious mononucleosis caused by Epstein-Barr virus].

Aim: Explore the immunological parameters, levels of activity of NAD(P)-dependent dehydrogenases lymphocytes, interferon status parameters, phagocytic activity and chemiluminescence response of neutrophils in the blood of children in the acute phase of infectious mononucleosis caused by the Epstein-Barr virus.

Patients And Methods: 65 children at the age of 4-6 years old with infectious mononucleosis caused by EBV in acute phase were observed. Such indexes as cell-mediated, humoral and interferon immunity, NAD(P)-depended dehydrogenases activity in blood lymphocyte, phagocytes activity, levels of spontaneous and induced chemiluminescence ofperipheral blood neutrophils were studied.

Changes in blood lymphocyte enzyme profile in patients with renal cancer after a course of interferon therapy.

Activities of NAD(P)-dependent dehydrogenases in blood lymphocytes of patients with renal cell cancer were studied. The enzyme profiles in patients were different before and after the course of immunotherapy. A relationship between changes in the enzyme status and in vitro cell sensitivity to IFN-α2a was detected.

Enzymatic status of blood lymphocytes in young children with Epstein-Barr virus infection.

Activities of NAD(PH)-dependent dehydrogenases in peripheral blood lymphocytes were studied in children aged 1-3 years in the dynamics of the disease caused by Epstein-Barr virus. A relationship between changes in activities of the studied enzymes and disease period was revealed. The disorders of blood lymphocyte enzymatic status persisted during convalescence.

Lucigenin- and luminol-dependent chemiluminescence of blood neutrophils in patients with renal cancer.

High basal production of primary active oxygen forms was detected in the peripheral blood neutrophils of patients with renal cell cancer. In vitro stimulation of neutrophils led to more rapid release of superoxide radicals into extracellular space and to a reduction of cell capacity to more intense production of primary active oxygen forms.

[Characteristics of lymphocyte enzymatic activity in children with Epstein-Barr infection].

Activity of NAD(P)-dependent dehydrogenases in peripheral blood lymphocytes from 140 children aged 1-14 years with Epstein-Barr infection was studied. It was shown to depend on the disease duration and children's age. Disturbances of lymphocyte enzymatic activity persisted during the reconvalescence period.

[Characteristics of enzymatic state and functional activity of immunocompetent cells in peripheral blood of patients with kidney cancer].

Characteristics of T-cell, humoral component of the immune system, activity of NAD(P)-dependent dehydrogenases in blood lymphocytes, phagocytic activity, and chemiluminescent response of blood neutrophils from patients with renal cell cancer have been examined before and within 14 days after surgical treatment. Changes of these parameters depended on the time elapsed after surgery. It is shown that immune status, enzymatic activity of lymphocytes and functional state of neutrophils in patients with renal cell cancer failed to reach normal levels 14 days after complete nephrectomy.

Evaluation of the expression of early activation marker CD69 by CD8+ lymphocytes in HIV-infected patients.

We studied the expression of CD69 antigen on CD8+ lymphocytes in response to nonspecific activator phytohemagglutinin in 202 patients with HIV infection. It was found that the number of CD8+CD69+ lymphocytes in HIV-infected patients increased; this parameter negatively correlated with relative and absolute content of CD4+ cells and positively correlated with IgG concentration.

Cell sensitivity to reaferon in patients with renal cell cancer after reaferon therapy.

Individual sensitivity of blood neutrophils to IFN-alpha2a was studied in vitro in patients with renal cell cancer before and after the first course of reaferon therapy. Cell sensitivity changed after reaferon therapy in 85% patients. This necessitates evaluation of individual cell sensitivity not only before therapy, but also before the next course of therapy.

[Clinical and immunological effect of vaccination against Haemophilus influenzae, type B, in children with disorders of the nervous system].

Observations on 277 children aged 3 months to 4 years with lesions of the nervous system, immunized with vaccine Act-HIB (Aventis Pasteur, France), were carried out in groups of children. The significant decrease of the level of Hib nasopharyngeal carrier state in 3.3 times and the decrease of total morbidity in acute infections of respiratory and ENT organs in 2.

[The clinical value of immunological markers of EBV infection in children with infectious mononucleosis].

A hundred and sixteen patients aged 1 to 14 years who had infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV) were followed up in its acute phase and 3, 6, and 12 months after the disease experienced by them. The clinical significance of the markers of EBV infection was assessed in children with IM. The high diagnostic capacity of detection of EBV DNA in the lymphocytes was established in patients with IM.

[Correlation of clinical manifestations of atopic dermatitis in children and the activity of NAD(PH)-dependent dehydrogenases in blood lymphocytes].

The bioluminescence was used to study the activity levels of NAD (P)-dependent dehydrogenases in blood lymphocytes of 88 children with atopic dermatitis. The nature and intensity of the investigated enzymes were found to be dependent on age and a disease severity with changes being most pronounced and resistant in the elder age group and in patients with a continuously relapsing disease course. Besides, it was shown that the changes of NAD (P)-dependent dehydrogenases in blood lymphocytes are objectively related with an allergic inflammation clinical course in patients with atopic dermatitis and could be clinically valuable in monitoring the dermatitis progression at the metabolic level, in prognosticating possible relapses and in evaluating a chosen therapy.

[The structural and functional status of the immune system in pre-school children infected with Mycobacterium tuberculosis].

Sixty children infected with Mycobacterium tuberculosis (MBT), aged 3 to 5 years were followed up in the period before specific chemotherapy. The studies established structural and functional changed in the immune system in young MBT-infected children. Moreover, the disorders found in immune homeostasis were of combined nature.

[Comparative analysis of the functional activity of neutrophil granulocytes from blood of patients with renal cell carcinoma before and after the surgical treatment].

Patients with renal cell carcinoma (RCC) were examined by the chemiluminescent analysis before and after surgery. The cells of subjects with the discussed pathology were found to be in the state of functionally metabolic misbalance, i.e.

[Evaluation of the metabolic state and functional activity of immunocompetent cells in children with relapsing herpetic viral infection].

Specific features of the immune response were studied (during the clinical remission period) in 40 children (aged 3 to 6) with a relapsing herpes viral infection (RHVI). The following was established: an increased relative and absolute quantity of lymphocytes, a reduced percentage content of CD+ and an increased absolute count of CD+ in the peripheral blood. Changes in the immunity humoral chain manifested themselves by an increased content of class G immunoglobulins and of circulating immune complexes (CIC) in the blood serum.

[Specific features of functional activity of neutrophilic granulocytes in infection with Mycobacterium tuberculosis in children of different age groups].

Sixty children infected with Mycobacteria tuberculosis from two age groups (3-5 and 12-14 years) were followed up. The phagocytic activity and oxygen-dependent biocidicity of peripheral neutrophilic granulocytes were studied. The studies indicated dysfunctions of neutrophilic granulocytes in the children infected with Mycobacteria tuberculosis.

[Metabolic characteristics of immunocompetent cells in children with relapsing herpes].

Healthy children aged 1-6 years and patients of the same age with recurrent herpesvirus infection (RHVI) during remission were examined. The levels of NAD(P)-dependent dehydrogenases of blood lymphocytes were measured. Lymphocyte metabolism in the patients was characterized by activation of the key reaction of the pentose phosphate cycle, increased level of the mitochondrial compartment oxidoreductases, increased substrate flow through glucose-3-phosphate dehydrogenase, and inhibition of NADH-dependent dehydrogenase reactions of the cytoplasmatic compartment.

[Diagnostic significance of chemiluminescent response of neutrophil granulocyte in children with lymphadenopathy].

Chemiluminescent response of the blood neutrophilic granulocytes was evaluated during the diagnostic period in 22 patients with Hodgkin's disease, 35 with nonspecific lymphadenopathies, and 24 patients with tuberculous infection aged 7-14 years. Disorders in spontaneous and induced chemiluminescence of neutrophils were detected in all patients, and were the most pronounced and stable in patients with Hodgkin's disease. Evaluation of neutrophilic chemiluminescent response in these patient groups leads to a more accurate diagnosis of lymphadenopathies of different origin.

[Interconnection of immunologic indicators of blood and functional parameters of leukocytes in children with relapsing herpesvirus infection].

Correlations between immunological indices of blood and functional activity of leukocytes were studied in healthy children and those with recurrent Herpes virus infection (RHVI) at the age 3-6 and 7-11 years. Functional activity of leukocytes was assessed by the chemiluminescence test. Both healthy and RHVI children are shown to have age-specific features of blood immunology, leukocyte function and their correlation.

[Clinical significance of NADPH-dependent dehydrogenases in lymphocytes from patients with bronchial asthma].

The activities of lymphocyte NADP-dependent dehydrogenases were evaluated in 84 children with atopic asthma during relapses and clinical remission. The pattern and severity of changes in the studied enzymes depended on children's age and duration and severity of disease. Changes in the older age group and during relapse were the deepest and most stubborn.

[A chemiluminescent analysis of the functional activity of the blood leukocytes in children with recurrent obstructive bronchitis and atopic dermatitis].

To study the informative value of chemiluminescence determination of leukocytic functional activity, children aged 3 to 6 years who had recurrent obstructive bronchitis (ROB) and atopic dermatitis (AD) were examined. The mean statistic data on spontaneous and induced chemiluminescence responses were found to be far from fully reflecting the individual characteristics of leukocytic functional activity in children with these diseases (in a group of patients with the same disease, children were found to have lower and higher values of cell chemiluminescence). This suggests that in developed infection, the functions of phagocytic cells may be not only activated, but also suppressed.