New Frontiers in Diagnosis and Therapy of Circulating Tumor Markers in Cerebrospinal Fluid In Vitro and In Vivo.

One of the greatest challenges in neuro-oncology is diagnosis and therapy (theranostics) of leptomeningeal metastasis (LM), brain metastasis (BM) and brain tumors (BT), which are associated with poor prognosis in patients. Retrospective analyses suggest that cerebrospinal fluid (CSF) is one of the promising diagnostic targets because CSF passes through central nervous system, harvests tumor-related markers from brain tissue and, then, delivers them into peripheral parts of the human body where CSF can be sampled using minimally invasive and routine clinical procedure. However, limited sensitivity of the established clinical diagnostic cytology in vitro and MRI in vivo together with minimal therapeutic options do not provide patient care at early, potentially treatable, stages of LM, BM and BT.

[The prophylactic effect of negatively charged air ions in acute stress in rats with different typological behavioral characteristics].

Typological characteristics of behaviour of 63 male white rats weighing 200-250 g were estimated by their locomotion in the open field. Acute stress was induced by putting the rats into narrow tubes for 1 h. Prior to the experiment, the rats were exposed to the air ions produced by Chizhevskiĭ air ionizer ("Elion-132") for 2 h daily within a week.

[The normalizing effect of air ions on neuroticized rats with different typological behavioral characteristics].

The work was carried out in 98 white male rats weighting 200-250 g. Typological properties of behaviour were estimated by locomotor activity in the open field. Neurosis was induced by electro-pain irritation (15 min daily during 3 weeks), accompanied by the white noise (4 h daily).

[The effect of chronic stress, long-term adaptation to hypoxia and their combination on the behavior of rats with different typological characteristics].

Chronic stress caused by 3-week electro-pain stimulation (4 h daily) combined with "white noise", resulted in a decrease of horizontal motor activity of rats with extreme types of behaviour, i.e., active and passive.

[Apha-tocopherol in a complex with dimethyl sulfoxide--an agent possessing highly effective adaptogenic action in chronic emotional-pain stress in rats].

Alpha-tocopherol (5 mg/kg) administered perorally with dimethyl sulfoxide (50 mg/kg) in chronic emotional pain stress in rats possesses an effective antistress action, exceeding the effects of these drugs administered separately. Their prophylactic complex administration prevents the hypertension produced by stress, disturbance of reactivity of the vegetative nervous system during functional load, change of the behaviour in the open field. Adaptogenic action of the drugs is accompanied by a reduction of the content of free-radical oxidation products and by raising of superoxide scavenging activity in the brain and blood serum, by raising of phospholipids content, lowering of cholesterol content and of the ratio of cholesterol phospholipids in the brain extracts.

[Antistress effect of dimethyl sulfoxide in the rat].

Administration of scavenger of hydroxyl radicals--dimethylsulfoxide (1 g/kg intraperitoneally, daily for 3 weeks) did not lead to any significant changes in animals behaviour in the open field and in visceral functions (arterial pressure, respiratory rate, heart rate) but prevented shifts of these characteristics caused by chronic 3-week emotional-pain stress. In rats injected with dimethylsulfoxide, an increase was observed of superoxide dismutase activity in the brain and blood serum. Molecular mechanisms are discussed of antistress action of dimethylsulfoxide (scavenge of hydroxyl radicals, activation of superoxide dismutase) and possible role of hydroxyl radicals in realization of damaging action of stress on the organism.