PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells.

High-throughput ribosome profiling demonstrates the translation of thousands of small open reading frames located in the 5' untranslated regions of messenger RNAs (upstream ORFs). Upstream ORF can both perform a regulatory function by influencing the translation of the downstream main ORF and encode a small functional protein or microprotein. In this work, we showed that the 5' untranslated region of the PRPF19 mRNA encodes an upstream ORF that is translated in human cells.

Express Diagnostics of Proteolytic Activity of Periodontopathogens-Methodological Approach.

The species spectrum of the oral microbiome is considered to be the key factor in the development and progression of periodontal inflammatory disorders. The “red complex” including Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola has the highest pathogenic potential. These bacteria have several biochemical mechanisms that allow them to colonize and destroy periodontal tissues.

[Computed tomography in determining the differentiation of ductal adenocarcinoma of pancreatic head].

Objective: To evaluate the features of «hypervascular rim», tumor dimensions and density as prognostic factors of differentiation of pancreatic head adenocarcinoma.

Material And Methods: Pancreatoduodenectomy was performed in 311 patients with pancreatic head adenocarcinoma for the period 2013-2019. A retrospective study included 81 patients who met the following criteria: available data of morphological and immunohistological examination indicating tumor grade from Grade 1 to Grade 3, as well as available preoperative CT images in four phases (native, arterial, portal and delayed).

[Central hemodynamics during conduction anesthesia in patients with pyonecrotic forms of the diabetic foot].

Conduction anesthesia-induced central hemodynamic changes were studied in 20 patients with pyonecrotic forms of the diabetic foot and coronary heart disease (CHD). There was a 19% reduction in cardiac output and a 25% increase in total peripheral vascular resistance, as compared with the baseline values, the level of blood pressure being unchanged. The authors proposed to include droperidole into premedication and intraoperative perlinganite infusion for postload reduction, which stabilized central hemodynamics during conduction anesthesia in patients with diabetes mellitus and CHD.

[Regional anesthesia and timely treatment for neuropathy in patients with pyonecrotic forms of the diabetic foot].

Regional anesthesia was studied for its impact on the functional status of a peripheral nerve in 20 patients with purulent diseases of the lower extremities in the presence of diabetes mellitus. The impact of conservative therapy on the peripheral nerve was also studied in these patients. There was no negative impact of regional methods of anesthesia on the function of the nerve, its improvement due to treatment with alpha-lipoic acid and serotonin adipinate.

[Evaluation of the clinical status of patients with surgical infection].

The possibility of objectively evaluating the severity of the clinical status of patients with surgical infection is discussed. A retrospective and prospective analysis of clinical laboratory values is carried out in 196 patients with surgical sepsis. SAPS score indicated a statistically significant difference in the state of two groups of patients (survivors and dead).

[Health care standards for surgical patients in clinical hospital].

In Russian Federation the Obligatory Health Insurance Standards are based on the a priori expert assessments but not on statistical analysis of really recorded cases. This leads to the essential standard incompleteness, indistinctness and heterogeneity. In Vishnevsky Institute of surgery the "Automatized system for care registration in hospital" was designed, and data base on more than 10,000 patients treated in 1995 to 1999 was created giving now the starting-point for further efforts for overcoming the above standards insufficiency.

[Current methods of morphological and hemostasiological analysis of reparative process in wounds making use of information programs].

Objective rapid morphological and hemostasiological methods for evaluating the reparative process in the wound making use of PC software have been developed for identification of stages of reparative process in the wound under conditions of modern surgical service. Cytran consultative software has been developed for evaluation of phases of reparative reaction in the wound. Coagulogram consultative software was used for identification of reparative reaction phases in the wound from hemostasiological changes in tissue factors of granulation tissue in the wound.

[Clinical and laboratory study of cefodizime (Modivid) in prophylaxis and treatment of surgical patients].

The results of the clinical and laboratory study of the efficacy of the prophylactic and therapeutic use of cefodizime (modivid) in patients with chronic calculous cholecystitis and burns are presented. Expediency of the preoperative prophylactic use of the drug and its significance in the treatment of infectious complications of the burn disease were verified. The immunological investigation gave evidence of an increase of the phagocytosis functional activity when cefodizime (modivid) was used prophylactically in cholecystectomy.

[Late results of lymphovenous anastomoses in lymphedema of the lower extremities].

The authors examined 152 patients with lymphedema of the lower limbs 2-6 years after operation for lymphovenous anastomoses (LVA). They evaluated the dynamics of edema according to Alberton's formula, the changes in the character of the episodes of erysipelas, and the patient's feeling of heaviness in the involved limb. The function of the anastomosis was studied by the radioisotope method.

[Myocardial asynergy of the left ventricle in patients with aortic stenosis].

Left ventriculograms obtained from 21 patients without any intracardiac hemodynamic abnormalities were analyzed by using the correlation method. The analysis revealed three functional areas of the left ventricle and defined normal values of their systolic and diastolic pulses. Hypo- and hyperkinesia of the functional areas in the left ventricle were quantitatively determined.

[Place of multivariate statistics in clinical physiological research].

On the basis of their own experience of many years in the use of mathematical methods in medicine the authors provide a critical analysis of the results of using computers in making the diagnosis and the main reasons for dissatisfaction with the results. Regarding clinico-physiological studies as a field for the application of modern mathematical statistics, they suggest a logical scheme which they had tested time and again for statistical analysis of the data by multidimensional methods (particularly with the use of the method of principal components, cluster analysis, latent analysis, lambda-moments, etc.) and also some methodological devices which permit keeping the data at hand throughout the entire analysis, resorting time and again to all possibilities for their complete and exhaustive description.

[Diagnosis of thrombosis of the left atrium in rheumatic heart defects, using the main components methods].

A new method of diagnosis of left atrial thrombosis was developed for rheumatic heart disease patients. The mathematic method of the main components was used that permits to detect the thrombosis of the left atrium in 94.3% of the cases.

[The character of the background spike activity of cortical neurons under the influence of psychotropic drugs].

A comparison was made on alert rabbits between the nature of spike activity of normal cortical neurones and of those after a two-week daily administration of neuroleptics, namely chlorpromazine, trifluoperazine and haloperidol in 1 and 5 mg/kg doses. The groups of neurones did not differ in the mean frequency of firing. However, the use of the main components method and of cluster analysis showed considerable differences between neuronal activity following the action of neuroleptics and that in control animals.

[Classification of cortical neurons according to the character of their background impulse activity].

The method of basic components and cluster analysis was used to classify 75 units in the visual cortex of alert rabbits, proceeding from the empiric form of distribution of interspike intervals (DII), the mean frequency of impulsation and the relative number of intervals up to 500 msec. They were classified into nine groups containing from two to twelve units. Besides the cues, used as a basis for classification, the groups of units also differed in the structure of burst activity, the correlation of adjacent intervals and the ratio between the short (up to 20 to 60 msec) and long intervals.