The in vitro activity of selected mouthrinses on Candida strains isolated from the oral cavity.

The purpose of this in vitro study was to determine the effects of 13 mouth rinses on one hundred Candida strains isolated from the oral cavity of patients. Five of the rinses had no antifungal activity. Chlorhexidine, Colgate and Dentosept acted on all isolated strains; Corsodyl did not act on C.

The in vitro activity of selected mouthrinses on the reference strains of Trichomonas tenax and Entamoeba gingivalis.

Protozoa, such as Trichomonas tenax, Entamoeba gingivalis and Leishmania braziliensis, may be present in the mouth but their role in the pathophysiology of oral diseases is not clear yet. The use of various types of mouthrinses plays an important role in maintaining proper oral hygiene and in removing some of the microbial components from the oral cavity. The purpose of this study was to investigate the effects of selected mouthrinses on the reference strains of Trichomonas tenax and Entamoeba gingivalis which can be a part of the oral cavity microbiota.

Drug susceptibility of fungi isolated from ICU patients.

Candida species can be a reason of infections associated with high morbidity and mortality. The risk of invasive candidosis for patients admitted to intensive care units (ICUs) is increased due to immunosuppressive states, prolonged length of stay, broad-spectrum antibiotics and Candida colonization. The aim of the study was to determine selected properties of fungi isolated from patients treated in the ICUs of hospitals in Lodz.

Prevalence of fungi in cases of geographical and fissured tongue.

One of the most common malformations of the tongue is fissured tongue, very often, is accompanied by geographic tongue; the etiopathogenesis of them is not known. The aim of study was to evaluate the prevalence of fungi in cases of fissured and geographic tongue, and to determine their species and susceptibility to selected antifungal drugs. The study included 104 patients with fissured and/or geographical tongue.

Minimal inhibitory concentration (MIC) of caspofungin and itraconazole inhibiting growth of Candida strains calculated from the linear regression equation.

Purpose: The aim of the study was to compare the susceptibility of Candida species to caspofungin and itraconazole.

Material And Methods: 118 strains of Candida species were used in the study: 8 pattern strains and 110 strains isolated from different ontocenoses. The susceptibility of fungi strains to drugs was determined by diffusion in agar gel.

Cigarette smoking induces overexpression of c-Met receptor in microvessels of oral lichen planus.

Introduction: Cigarette smoking is related to many pathological conditions; however, chemical substances affect the oral cavity first, so it is important to consider its influence on oral mucosa and oral potentially pre-malignant lesions. The aim of this study was to investigate the effect of smoking on microvessel density in oral lichen planus. Special emphasis was placed on examining the relationship between the expression of c-Met receptor in blood vessels and smoking habits.

Expression of Th1/Th2/Th3/Th17-related genes in recurrent aphthous ulcers.

The pathogenesis of recurrent aphthous ulceration (RAU) is unknown, although an abnormal immune reaction appears to be involved. RAU may result from oral epithelium damage caused by T cell-mediated immune response. To improve understanding of the role of T cells in RAU, the present study analyzed.

Knowledge on Lyme disease among foresters.

Objectives: The first aim was an attempt to evaluate the level of knowledge on Lyme disease among people whose profession involves working in the forest; the second--recognition of the health problems that should be included in health education programmes concerning Lyme disease in this group of professionals.

Materials And Methods: The study was performed on 159 subjects.

Results: Only 15% know the etiological factor of disease, 98%--the main cause of infection, and route of pathogen transmission.

[Interspecies differentiation of Candida albicans (Robin, 1853) Berkhout, 1923 strains from multifocal invasions--their identity or similarity parameters].

The subject of analysis is a pathogenic species of fungi that has the highest prevalence in the word--Candida albicans. The aim of this study was to determine the identity and similarity of strains for the diagnosis of multifocal (concerning two or more organs) invasions. The material was comprised of 210 strains isolated from 83 women from 6 various ontocenoses: in group I--from the vagina, oral cavity and rectum, in group II--from the vagina and oral cavity, in group III--from the oral cavity, oesophagus, stomach, duodenum and rectum.

[The immune response to fungal infections].

In mycoses congenital--nonspecific innate as well as acquired immunity (involving neutrophiles, monocytes, macrophages, dendritic cells and lymphocytes) both play important roles in host defence. Th1 lymphocytes release cytokines (IL-2, IL-12, IFN gamma) and stimulate cytotoxic cells and neutrophiles to destroy fungal cells. Th2 lymphocytes, on the other hand, suppress cellular immunity by releasing the cytokines IL-4, IL-6 and IL-10 which counter regulate the secretion of IL-2, IL-12, IFN gamma and depress the activity of macrophages.

[Susceptibility to miconazole and itraconazole of Candida strains isolated from hospitalized and outpatient clinic patients].

It is known that fungi representing different genera and species can cause organ-limited or systemic infections after disrupting of the natural defense mechanisms in a human organism. The treatment of mycoses still encounters considerable difficulties. Therefore, in vitro assessment of the susceptibility of fungal strains to the antifungal agents now in use and to new drugs is needed more urgently than ever before.

[The diagnostic methods applied in mycology].

The systemic fungal invasions are recognized with increasing frequency and constitute a primary cause of morbidity and mortality, especially in immunocompromised patients. Early diagnosis improves prognosis, but remains a problem because there is lack of sensitive tests to aid in the diagnosis of systemic mycoses on the one hand, and on the other the patients only present unspecific signs and symptoms, thus delaying early diagnosis. The diagnosis depends upon a combination of clinical observation and laboratory investigation.

[Occurence of fungi in oral cavity of patients with periodontitis].

The role of fungi in the etiology and pathogenesis of periodontal diseases is unclear. What more, there exist only few studies concerning occurrence of fungi in the oral cavity of patients with periodontitis. The aim of the study was to assess the frequency of fungi occurrence and to search relationship between its presence and the state of paradontium, indices of oral hygiene as well as to evaluate periodontal treatment needs.

Dysfunction of CD4+CD25high T regulatory cells in patients with recurrent aphthous stomatitis.

Background: Recurrent aphthous stomatitis (RAS) is a chronic inflammatory disease of unknown etiology characterized by recurring formation of painful oral ulcers. RAS may result from oral epithelium damage caused by T-cell-mediated immune response. CD4(+)CD25(+) T regulatory (Treg) cells suppress proliferation and effector functions of other immune cells, and therefore are crucial in regulating the immune response.

[Treatment of fungal infections of upper respiratory tract and ear].

Fungi, in comparison with other pathogenic factors, have high pathogenicity. The number of fungal species which are able to infect people is over 500. The upper respiratory tract and ear have permanent contact with external environment which makes their ontocenoses open to continuous exchange of microorganisms of which they consist.

[Clinical evaluation of periodontium in pregnant women with risk of preterm birth].

Objectives: The aim of the study was to evaluate condition of the periodontium in pregnant women with pathological progress of the pregnancy, clinically and to compare it to periodontium in pregnant women in good health.

Design: Over the last years, the studies have described that periodontitis caused by dental plaque, could be the risk factor for preterm birth and low birth weight.

Materials And Methods: This study was performed in 80 pregnant women, 40 with pathologic pregnancy and 40 with normal pregnancy in it.

Search for the pathogenic fungi in amniotic fluid.

Intra-amniotic infection with Candida is rare but considers an etiological factor for preterm rupture of membranes (PROM). The aim of the study was the investigation of the mycotic invasion of the amniotic cavity in pregnant women with PROM and with intact fetal memranes. 170 women were included in two groups: I (n = 150)--women with intact membranes; II (n = 20)--women with PROM.

[Some aspects of fungi multifocal invasions connected with the genital organs in women].

The aim of presented study was analysis of fungal invasions of the genital organs, oral cavity and digestive tract in 799 women. Fungal strains were identified using API 20C and API 20C AUX (bioMérieux); they were as follows: Candida albicans, C. krusei, C.

[Susceptibility to itraconazole and miconazole of fungi strains isolated from the vaginal ontocenosis].

The activity of itraconazole (RO 51211, molecular weight 705,64) and miconazole (RO 14889, molecular weight 479,15) obtained from Janssen Pharmaceutica NV, against 100 fungi strains, was studied. These strains were isolated from vaginal materials of patients with recognized candidosis. The axenic fungal strains were assessed by own methods and unified tests (bioMérieux: API 20 C, API 20 C AUX).

[Proteolytic activity of Candida albicans strains isolated from women's genital organs with clinical diagnosis of mycosis].

The aim of the presented study was to assess the in vitro activity of aspartate proteinase of strains isolated from women's genital organs with clinical diagnosis of mycosis. We have evaluated the activity of proteinase of 75 Candida albicans strains using Staib method with Rózga modification, in agar medium with human albumin at pH 4.6.

[Multifocal fungal infections in pregnant women].

The aim of the study was to estimate the prevalence of fungi in monofocal and multifocal infections in pregnant women. 251 pregnant women were examined for presence of fungi in vagina, oral cavity and rectum. Fungal strains were recovered in 259 out of 753 samples (49.

[Prevalence of Toxoplasma gondii and analysis of specific immunoglobulins concentration in serum of women during the reproductive period in a sample of Włocławek population].

The aim of the study, from 1996 to 1999, was to investigate the serological course of prevalence Toxoplasma gondii in 985 women of procreation period. In non - pregnant women the incidence of Toxoplasma gondii specific IgG was significantly higher (59.0 +/- 1.

Serodiagnostics of Toxoplasma gondii invasions.

Diagnosis of T. gondii invasions is based mainly on serological tests, because of their relative simplicity and availability. In the present work we examined efficiency of immunoblot and two inhouse ELISA assays in anti-Toxoplasma antibody detection, comparing our results with those obtained using commercial ELISA kit of Organon Teknika.

[Hydrolytic activity of Trichomonas vaginalis Donné strains].

The hydrolytic activity of Trichomonas vaginalis Donné strains was investigated using API ZYM test (bioMérieux). Among 19 determined hydrolases were found alkaline and acid phosphatases; also in several strains N-acetyl-beta-glucosaminidase and a-glucosidase were demonstrated. The API ZYM test may be used for characterization of enzymatic activity of T.

[Prevalence of Trichomonas vaginalis Donné in women of Lódź population in 1955-1999 years].

Search for Trichomonas vaginalis was carried out in direct (in 0.85% NaCl and 0.1% safranine) and fixed stained microscopical preparations ( Wright or Giemsa methods, after fixation with 70% methyl alcohol) and cultures ( Pavlova, Johanson-Trussel-John, Roiron-Ratner, Simic media), from materials of the vagina, urethra and cervix uteri.