[A case of Creutzfeldt-Jakob disease with markedly elevated ubiquitin concentration in the cerebrospinal fluid].

A 56-year-old woman initially noticed dizziness in October, 1988, and later dementia and gait disturbance developed, associated with myoclonus and periodic synchronous discharge in the electroencephalogram. On the basis of these clinical findings we made a diagnosis of Creutzfeldt-Jakob disease (CJD). Using RIA for ubiquitin (signal peptide of the ATP dependent proteolytic system), we measured the cerebrospinal fluid (CSF) ubiquitin levels.

Calcitonin gene-related peptide immunoreactivity in spinal spheroids in motor neuron disease.

The spinal cords from seven autopsy cases of sporadic motor neuron disease (MND) and two controls were immunohistochemically examined using antibodies directed to calcitonin gene-related peptide (CGRP) and to neurofilament proteins (Nf). CGRP immunoreactivity was observed in the posterior horns, especially in the laminae I and II, of all the spinal cords examined. In MND cases, in addition, a considerable number of spheroids in the anterior horns were labelled with the antibody.

Sideways and rotational displacement of the temporomandibular joint disk: diagnosis by arthrography and correlation to cryosectional morphology.

Arthrography and arthrotomography were performed on 29 fresh temporomandibular joint autopsy specimens to diagnose medial and lateral disk displacement. A horizontal contrast medium margin crossing over the condyle (edge sign) and the relative size of the anterior recess of the lower joint compartment in the lateral and medial parts of the joints were used as indicators of the mediolateral position of the disk. Arthrographic findings were compared with coronal cryosections in a blinded fashion.

Osteoplasty of the mandibular condyle with preservation of the articular soft tissue cover: comparison of fibrin sealant and sutures for fixation of the articular soft tissue cover in rabbits.

Fibrin sealant and sutures were compared for fixation of the articular soft tissue cover after it had been raised in association with osteoplasty of the mandibular condyle. Nine adult rabbits were operated on bilaterally with the use of fibrin sealant on one joint and sutures on the other joint. The rabbits were killed after 3 months.

Temporomandibular joint: variation of normal arthrographic anatomy.

The arthrograms of 34 individuals with asymptomatic, clinically and arthrographically normal temporomandibular joints were analyzed. The results showed a substantial variation of the dimensions and configuration of the joint compartments. Thus the anterior recess of the lower joint compartment was small in 15 joints (44%), medium-size in 16 joints (47%), and large in three joints (9%).

High condylar shave of the temporomandibular joint with preservation of the articular soft tissue cover: an experimental study on rabbits.

The high condylar shave has the disadvantage of removing the articular soft tissue cover together with the underlying condylar bone. In an attempt to overcome this disadvantage, we elevated the articular soft tissue cover, shaved the condylar head, and replaced the articular soft tissue cover in its original position in nine adult rabbit temporomandibular joints. On the contralateral joints, the same amount of bone was shaved together with the articular soft tissue cover.

Reliability of a negative clinical temporomandibular joint examination: prevalence of disk displacement in asymptomatic temporomandibular joints.

Unilateral temporomandibular joint arthrography was performed in 40 healthy volunteers with asymptomatic and clinically normal temporomandibular joints. Thirty-four joints (85%) showed superior disk position, and six joints (15%) were radiographically abnormal with displacement of the disk. Thus, two showed anterior displacement, one showed rotational anterolateral displacement, one showed rotational anteromedial displacement, one showed sideways lateral and one showed sideways medial displacement.

Cryosectional observations of functional anatomy of the temporomandibular joint.

The anatomy and function of the temporomandibular joint are complex, and our understanding about how the retrocondylar tissues change when the condyle translates anteriorly has been incomplete. We therefore examined the sectional anatomy of 24 fresh human temporomandibular joints at different gradations of jaw opening, protrusion, and laterotrusion. We found that the tissues of the posterior disk attachment expanded in volume and occupied the space behind the condyle when the mouth was opened, protruded, or laterotruded to the contralateral side.

Arthroscopic diagnosis of perforation and adhesions of the temporomandibular joint: correlation with postmortem morphology.

Arthoscopy was performed in the upper joint compartment of 28 temporomandibular joints in 16 fresh cadavers to investigate the accuracy of arthroscopic diagnoses of perforation and adhesion. Subsequent dissections revealed that only three of nine perforations were seen arthroscopically and that three of four joints with adhesions were arthroscopically diagnosed. There was no overdiagnosis of perforation or adhesions.

Histologic features of the temporomandibular joint disk and posterior disk attachment: comparison of symptom-free persons with normally positioned disks and patients with internal derangement.

To gain further knowledge about the differences between normal and pathologic anatomy of the temporomandibular joint disk, we examined histologically disks obtained at autopsy from 10 symptom-free persons and compared our findings with observations involving 17 surgically removed disks. The surgical patients had internal derangement and severe long-standing temporomandibular joint pain and dysfunction. The normal disks were biconcave, whereas the surgically removed disks were deformed and thicker than the normal disks.

MR imaging of the temporomandibular joint: comparison of images of autopsy specimens made at 0.3 T and 1.5 T with anatomic cryosections.

We made MR images of 39 autopsy specimens of the temporomandibular joint at 0.3 and 1.5 T and compared the images with anatomic cryosections.

Temporomandibular joint: MR assessment of rotational and sideways disk displacements.

The accuracy of coronal and sagittal magnetic resonance (MR) imaging was examined in the assessment of rotational and sideways displacements of the temporomandibular joint (TMJ) disk. Rotational disk displacement implies a combination of anterior and medial or lateral displacements, whereas sideways displacement implies pure medial or lateral displacement without an anterior component. Multiple 3-mm-thick coronal and sagittal MR images were obtained of 18 fresh TMJ autopsy specimens and compared with the observations in corresponding coronal cryosections.

A case of giant mediastinal leiomyoma with long-term survival.

A 67-year-old female was emergently admitted to our hospital because of severe dyspnea with cyanosis. One month before admission, she consulted a doctor and chest x-ray disclosed a huge tumor shadow occupying most of the left hemithorax. At that time, she did not complain of any particular symptoms except slight cough.

[Arterial infusion chemotherapy and embolization in unresectable hepatocellular carcinoma using an intraarterial catheter].

We performed arterial infusion chemotherapy and embolization on 22 patients with unresectable hepatocellular carcinoma via an intraarterial catheter, which was inserted in the hepatic artery under laparotomy. The administration frequency of intraarterial chemotherapy on a patient was 1 to 155 with a median of 17, while that of embolization was 1 to 6 with a median of 2.5.

A simple freezing medium for serum-free cultured cells.

Methylcellulose was found to protect serum-free cultured cells from the deleterious effects of freezing and thawing. We have formulated a simple medium suitable for freezing serum-free cultured cells that consists of 0.1% methylcellulose, 10% dimethylsulfoxide, and MEM or any other serum-free culture medium.

Stopped-flow X-ray scattering device with a slit-type mixer.

A slit-type mixer specially designed for stopped-flow X-ray scattering was constructed. It mixes two solutions at a time, allowing a large-sized window to be used immediately after mixing. Dead time of the mixer was 13 ms with 3.

Bony mass associated with hypertrophy of the sternohyoid muscle after radical neck dissection.

As a late complication after radical neck dissection, this paper reports a bony mass at the sternal end of the clavicle associated with hypertrophy of the sternohyoid muscle. The bony mass and hypertrophy of the muscle were considered due to continuous mechanical strain by the drooping of the shoulder caused by injury of the accessory and cervical nerves, left-handed physical labour, and the lack of the sternocleidomastoid muscle in rotating the head.