IMMUNOHISTOCHEMICAL ANALYSIS OF APPENDIX CELL WALL INFILTRATE IN ACUTE PHLEGMONOUS APPENDICITIS.

The purpse of the study was to analyze the content of the mucous infiltrate of cells of the lamina propria of the appendix using immunohistochemical analysis in patients with acute phlegmonous appendicitis. The research included 21 patients with acute phlegmonous appendicitis. The control group consisted of 15 persons lacking the inflammation of the intraperitoneal cavity.

[Antagonistic Properties of Microscopic Fungi Isolated from Plasterboard].

Aim: The aim was to study the antagonistic interactions between microscopic fungi that often contaminate plasterboard, and test cultures which recommended determining the fungus resistance.

Methods: Determination of interactions between test-cultures and strains isolated from plasterboard was carried out by agar block method.

Results: The isolated from plasterboard Alternaria infectoria F-41218 E.

Cellulase and Xylanase Activities of Endophytic and Soil Penicillium funiculosum Strains.

Aim: To carry out a comparative study of cellulase and xylanase activities of 16 endophytic and soil Penicillium funiculosum strains.

Methods: The objects of the study were 16 endophytic and soil P. funiculosum strains from culture collection of microscopic fungi of Department of Physiology and Taxonomy of Micromycetes of D.

[Screening of Mannane-Degrading Enzymes Producers].

The aim of research was to investigate the prevalence of complex mannan-degrading enzymes among museum and freshly isolated cultures of micromycetes, actinobacteria and bacteria. It has been shown that the producers of β-mannanases mostly extracted from sources that are rich in plant residues. We detected strains of Penicillium aculeatum, P.

[Screening of the Producents of α-L-Rhamnosidases Amongst Representatives of Penicillium].

The aim of this work was to study α-L-rhamnosidase [КФ 3.2.1.

[THE SPECIES COMPOSITION OF MICROMYCETES ISOLATED FROM PLASTERBOARD].

Aim: To investigate the mycobiota of usual and water-resistant plasterboard of two types and to represent its ecological characteristic.

Methods: Microscopic fungi were iso- lated using the method of modeling effects of humid air and water on the plasterboard, and methods of serial dilutions and direct inoculation on agar nutrient media. Analysis of mycobiota of different types of plasterboard was conducted using the methods of quan- titative ecology.

[GROWTH OF MICROMYCETES FROM DIFFERENT ECOLOGICAL NICHES ON AGAR NUTRIENT MEDIA].

Radial growth rate of (K(r)) 153 strains 6 species of micromycetes from different ecological niches was studied on 7 agar media: three standard (malt extract agar, potato-dextrose agar, Czapek's agar), and on agar media with plant polymers (carboxymethylcellulose, xylan, soluble starch and apple pectin). Endophytic and plant pathogenic strains (biotrophs) of all studied species did not differ significantly in their ability to grow on nutrient media of different composition--average values of K(r) for these two groups were the same (0,200 and 0,199 mm/h, respectively). Soil micromycetes (saprophytes) characterized by the lowest average growth rate (0,169 mm/h) and significantly differed from the endophytic and plant pathogenic ones.

[Biodegradation of agricultural plant residues by Fusarium oxysporum strains].

The cellulolytic and endoglucanase activity of Fusarium oxysporum strains isolated from soil and plants in the media with plant waste as carbon source has been studied. It was established that the majority of studied strains were able to hydrolyze the filter paper, husk of sunflower seeds, wheat straw and corn stalks. Cellulolytic activity depended on the strain of microscopic fungi, type of substrate and duration of cultivation.

[Growth characteristics of Fusarium poae (Peck) Wollenw. and Penicillium funiculosum Thom strains].

A comparative study of growth characteristics and peculiarities of glucose utilization by Fusarium poae and Penicillium funiculosum strains isolated from different habitats was conducted. It was found that F. poae and P.

[Cellulolytic activity of Fenellia flavipes and Fusarium oxysporum strains].

The production of cellulolytic enzymes by Fenellia flavipes and Fusarium oxysporum strains in submerged culture with plant residues as carbon source was studied. It was established that the majority of studied strains was able to hydrolyze the filter paper, husk of sunflower seeds, wheat straw and corn stalks. The ability to synthesize enzymes depended on the strain of microscopic fungi, type of substrate and duration of cultivation.

[Trichothecene mycotoxins of Fusarium poae from different habitats].

Comparative study of the ability of three strains of Fusarium poae for the synthesis of trichothecen mycotoxins has been carried out. Studied strains were isolated from different habitats: forest soil, wheat (plant pathogen) and cranberry root (endophytic strain). All three strains were able to synthesize T-2 toxin, HT-2 toxin and T-2 tetraol but they were in various amounts.

[Utilization of different carbon sources by soil and endophytic strains of Penicillium funiculosum Thom].

The ability of soil and endophytic strains of P. funiculosum to accumulate biomass under cultivation conditions in media containing carbon sources from mono- to polysaccharides has been studied. It has been shown that the most favorable carbon sources for the studied strains were maltose, sucrose, xylose, fructose, pectin, less assimilated lactose, arabinose, and especially microcrystalline cellulose.

[Polygalacturonase activity of microscopic fungi of different trophic groups].

A comparative study of polygalacturonase activity of 85 saprophytic, plant pathogenic and endophytic strains of Fusarium poae, Alternaria alternata, Penicillium funiculosum and Mycelia sterilia was conducted. It was established that in general polygalacturonase activity of Mycelia sterilia and P. funiculosum strains was significantly higher than that of A.

[Utilization of carbon sources by Fusarium poae (Peck) Wollenw. Strains from different trophic groups].

It was shown that saprophytic (soil), endophytic and plant pathogenic strains of F. poae under cultivation conditions in the media containing carbon sources from mono- to polysaccharides had different abilities to use them and to accumulate biomass. Maltose, xylose, fructose, pectin were the most favorable carbon sources for the studied strains; less assimilated lactose, arabinose, and especially microcrystalline cellulose were less assimilated.

[Amylolytic activity of Fusarium LK:FR. and Alternaria NEES:FR. species].

A comparative study of saprophytic, plant pathogenic and endophytic strains of different Fusarium and Alternaria species was conducted. It was shown that amylolytic activity of Fusarium strains of different trophic groups was low or absent. Plant pathogenic Alternaria spp.

[Xylanase activity of phytopathogenic and endophytic strains of Ceratocystis sp].

A comparative analysis of xylanase activity of 36 phytopathogenic and endophytic Ceratocystis sp. strains was conducted. The rate of their linear growth on the medium with xylan was studied.

[Mycobiota of Quercus robur L. from the oak forest in the Zhytomyr region].

Different organs of 155 samples of conditionally healthy and sick oaks were examined for mycological contamination. The isolated microscopic fungi belonged to 129 species of different taxonomic groups. The pathogenic complex was represented by 30 fungi species, however most of these species were isolated in single cases.

[Cellulase activity of Ceratocystis sp. of different trophic groups].

A comparative analysis of cellulase activity of 36 fungal strains of phytopathogenic and endophytic Ceratocystis sp. was conducted. The rate of their linear growth on the media with carboxymethylcellulose was studied.

Endoglucanase-producing micromycetes capable of plant wastes degrading.

The screening study of endoglucanase producers among 58 strains 28 species 12 genera of mesophilous and thermotolerant micromycetes has been performed. Approximately 20% of investigated strains of micromycetes showed the highest endoglucanase activities (hydrolysis rates reached 0.40-0.

[Cellulase and xylanase activities of Fusarium Lk:Fr. genus fungi of different trophic groups].

A comparative analysis of cellulase and xylanase activities of 26 fungal strains of phytopathogenic, saprophytic and endophytic Fusarium species has been realized using the qualitative reactions. The rare of their linear growth on the media with carboxymethyl cellulose or xylane has been studied. It was shown that the fungi of genus Fusarium belonging to different trophic groups possessed low activities of investigated enzymes as a whole, but in endophytic strains their levels were lower than in phytopathogenic ones.

[Cellulase and xylanase activity of phytopathogenic and endophytic fungal strains of Alternaria alternata (Fr.) Keissler].

A comparative analysis of cellulase and xylanase activity of 25 fungal strains of phytopathogenic and endophytic Alternaria alternata had been realized for the first time using the qualitative reactions. The rate of their linear growth on the media with carboxymethylcellulose or xylane had been studied. The cellulase and xylanase activities clearly depended on the distinct strain.

[Study of availability of some hydrolytic and redox enzymes in strains of Fusarium oxysporum (Schlecht.) Snyd. and Hans. isolated from different habitats].

The work authors have used 52 strains of F. oxysporum for the collection of cultures of the Department of Physiology and Taxonomy of Micromycetes of IMV of the NAS of Ukraine. The strains were isolated from three habitats--grain cultures, cultivated and noncultivated soils of different regions of Ukraine.