[Changes in growth patterns and intracellular calcium concentrations in aspergillus awamori treated with amphotericin B].

Growth patterns and intracellular Ca2+ concentrations in the mutant strain Aspergillus awamori 66A, containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin B. The cell response, i.e.

[Effect of a chemical analogue of autoinducers of microbial anabiosis on the Ca2+ response of mycelial fungi].

The microbial alkylhydroxybenzenes (AHB), autoinducers of anabiosis, or d1 factors, participate in stress response of mycelial fungi, as determined from changes in intracellular Ca2+ concentration. By using the genetically modified strain Aspergillus awamori 66A, which produces a recombinant Ca2+-dependent protein aequorin, the dynamics of Ca2+ was studied in the cytosol of cells exposed to mechanical shock in the presence of the protective doses (0.001-0.

[Analysis of the Ca2+ response of mycelial fungi to external effects by the recombinant aequorin method].

Using the mutant strain Aspergillus awamori 66A producing a recombinant Ca2+-dependent photosensitive protein aequorin, the dynamics of Ca2+ was studied for the first time in the cytosol of the micromycetes exposed to stressful factors, such as an increase in extracellular Ca2+ to 50 mM, hypoosmotic shock, and mechanical shock. Cell response to stress proved to involve an increase in the Ca2+ concentration in the cytosol, which was determined from the amplitude of aequorin luminescence and the time of the amplitude enhancement and relaxation. The level of Ca2+ response depended on the physiological stimulus.

[Dependence of the effect of RNAase from Bacillus intermedius on the growth of baker's yeast on the concentration of the exogenous enzyme].

Bacillus intermedius RNase added at a low concentration (0.001 microgram/ml) stimulated yeast growth, while a high RNase concentration (1500 micrograms/ml) was inhibitory to yeast growth. The inhibitory effect of RNase was transient and correlated with the increase in the trehalose pool of yeast cells.

[Effect of Bacillus intermedius ribonuclease on properties of the yeast Saccharomyces cerevisiae].

Effects of Bacillus intermedius ribonuclease on physiological, biochemical, and consumer properties of baker's yeast Saccharomyces cerevisiae were studied. This enzyme improved the yeast raising strength and increased the cell tolerance to various adverse factors. The antistress effect of RNase correlated with an earlier start of the stationary growth phase and increased trehalose pool.

[Stabilization of enzymes by anabiosis autoinducers as a possible mechanism of resistance of resting microbial forms].

Alkyl-substituted hydroxybenzenes (AHBs), auto-inducers of microbial dormancy (or d1 factors), were found to stabilize the structure of protein macromolecules, making them metabolically less active and more resistant to stresses. In vitro experiments with the Bacillus intermedius ribonuclease and chymotrypsin showed that the degree of the physical and chemical stability of these enzymes treated with AHBs depends on their concentration and incubation time. Experiments with RNase, which is capable of refolding, i.

[Changes in the trophic organization of mitotic cycle of Candida utilis after exposure to RNAse from Bacillus intermedius].

The effect of the RNase from Bacillus intermedius on the growth and trophic cycle of Candida utilis was studied. The RNase at concentrations of 0.001-0.

[Effect of the RNAase from Bacillus intermedius on growth and physiological characteristics of Escherichia coli].

The effect of the RNase from Bacillus intermedius on the growth of Escherichia coli was investigated. RNase added to growth medium enhanced the synthesis of DNA, RNA, and protein and stimulated cell division; the degree of stimulation depended on the enzyme concentration. A necessary condition for stimulation was the adsorption of the enzyme on the cell surface and its interaction with the cytoplasmic membrane, as demonstrated immunocytochemically.

[The use of pancreatic RNAse in the production of baker's yeast].

Microdoses of a preparation of pancreatic RNase were shown to stimulate the growth of Saccharomyces cerevisiae yeast. The effect was only retained at a certain inoculum/enzyme preparation ratio. Industrial tests of the preparation showed that the addition of RNase to the first reservoirs for culture accumulation (an inoculator and a seeding device) increased the yield of bakers' yeast and improved their quality.

[Use of exogenous ribonuclease for improvement of Lactobacillus biological properties].

The influence of exogenous RNAse on the dynamics of the acid formation by the industrial strain 8R-A3 of Lactobacillus plantarum, its antibiotic sensitivity and antagonistic activity was studied. In the presence of the RNAase growth stimulating dose both a decrease of the culture lag-phase and a more intensive accumulation of lactic acid in the incubation medium resulting in an increase of the Lactobacillus antagonistic activity were observed. It was shown that RNAase increased the Lactobacillus stability to tetracycline and erythromycin by 32 to 57 per cent as compared to the control.

[Effect of Bacillus intermedius RNAases with various catalytic activity on multiplication of Escherichia coli and Bacillus subtilis].

The effect of the Bacillus intermedius ribonuclease and its mutant forms derived by site-specific mutagenesis on the growth of gram-positive and gram-negative bacteria was studied. Both catalytically active and catalytically inactive (mutant) ribonucleases stimulated bacterial growth, the extent of stimulation correlating with the catalytic activity of the enzymes. It was suggested that the biological activity of exogenous ribonucleases is mainly due to their catalytic activity.

[The stimulating action of exogenous RNase on eubiotic strains of Lactobacillus, Bifidobacterium and Escherichia coli].

The influence of Bacillus intermedius RNAase on the multiplication on B.B.bifidum, L.

[Effect of Bacillus intermedius RNAase on growth of a Saccharomyces cerevisiae culture].

The effects of RNase from Bacillus intermedius on proliferation of Saccharomyces cerevisiae were studied. The enzyme (0.01 microgram/ml) stimulated the yeast cell budding.

[Change in various biological properties of Lactobacillus as affected by ribonuclease].

The influence of RNAse from Bacillus intermedius on the growth of the industrial strain Lactobacillus plantarum 8R-A3 was studied. It was shown that the stimulating effect of the enzyme depended on its dose and manifested itself in decreasing the growth lag phase. At the same time the growth stimulating dose of RNAse increased the Lactobacillus adhesion to the epithelial cells and promoted secretion of proteinases from Lactobacillus to the culture medium.

[An immunocytochemical study of bacterial ribonuclease penetration into Candida utilis yeast cells and the effect of the enzyme on yeast multiplication].

Permeability of Candida utilis cells for exogenic Bacillus intermedius RNase was studied cytochemically. The enzyme was shown to penetrate through the outer membrane of C. utilis after a 20 min incubation with cells being in exponential phase, and to be connected with the yeast cytoplasmic membrane.

[Effect of membranotropic physiologically-active substances on the growth of Saccharomyces cerevisiae cultures].

The effect of Bacillus intermedius RNase and yeast autoregulatory d2 factor on growth of Saccharomyces cerevisiae is reported. It is shown that 0.01 microgram/ml of RNase stimulates the growth of the yeast on malt wort and molasses, the peak of the effect being observed for the enzyme added in the middle exponential phase.

[The effect of Bacillus intermedius RNAase on the growth and development of Bacillus mucilaginosus].

The effect of Bacillus intermedius RNAase on vegetative cells and spores of siliceons bacteria Bacillus mucilaginosus was investigated. It is shown that the enzyme stimulates the growth of vegetative cells of B. mucilaginosus at a concentration of 10 mkg/ml and promotes germination of spores at a 1000-fold lesser concentration (0.

[Penetration of pancreatic DNase into Candida tropicalis cells].

Permeability of Candida tropicalis cells for exogenic DNAse was studied by a cytochemical method. The enzyme were shown to penetrate yeast outer membrane and cell wall after a 20 minute incubation period when incubated together with cells at the beginning of the stationary phase.

[The biochemical aspects of the stimulating action of exogenous RNAse].

A sequence of biochemical reactions in yeast from moment of RNAse interaction with cell membrane to cell division has been studied. RNAse addition in growth medium causes the increase of Ca2+ entering rate in cells in 2.4 times.

[The effect of Bacillus intermedius RNAse on the multiplication of Candida tropicalis yeasts].

The effect of Bacillus intermedius RNAse on the reproduction of Candida tropicalis and synthesis of the main biopolymers in the yeast cells. It has been found that stimulating action of the enzyme appears at the concentration of 10(-5)-10(-6) mg/ml and does not depend on the physiological state of the sowing culture. The connection between the increase of the ionic penetration and stimulation of the RNA and proteins synthesis in the yeast cells subjected to the RNAse action is shown.