[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].

Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested.

[Evaluation of morbidity with temporary work disability in a metal treatment enterprise].

The authors present materials about influence of occupational factors on transitory disablement morbidity, exemplified by metal treatment enterprise.

[Study of hepatitis E virus replication in FRhK-4 cell culture].

The cytolytic form of hepatitis E virus reproduction in FRhk-4 cells is described. The cytopathic effect was observed by days 6-7 of the 25th passage in this cell culture. Reverse transcription with polymerase chain reaction were used to study virus reproduction daily for 10 days postinfection.

[Neurovasal relations in the human neocortex].

Different types of interneurons producing nitric oxide in human brain motor cortex were studied by means of histochemical reaction to NADPH diaphorase--the marker of nitric oxide synthase. Non-synaptic dendrito-, axo- and somatovasal contacts between typical smooth and spinous checking interneurons were shown. A hypothetic scheme of local blood circulation control is represented, after which these cells, exerting inhibitory synaptic action on pyramidal neuron at the same time cause vasodilatation by secreting nitric oxide near to the capillary.

[The morphofunctional heterogeneity between peritoneal and alveolar macrophages in their biogenic amine content: the effect of their presence or absence in the microenvironment of mast cells].

Peritoneal macrophages differ from alveolar ones in biogenic amine content: peritoneal macrophages contain smaller amounts of histamine and serotonin. When activated, alveolar macrophages release biogenic amines, whereas in peritoneal macrophages stimulators of macrophage activity induce the increase in amine content. This may be due to the presence of mast cells in their microenvironment macrophage activity.

[The effect of immunomodulators that stimulate macrophagal functional activity on the morphofunctional characteristics of mast cells].

The influence of drugs stimulating macrophage activity (prodigiosan, leukinferon, alpha-interferon) on cytochemical properties of mast cell populations of gastric and jejunal mucosa (mucosal mast cells) and peritoneal fluid and mesenterial mast cells (connective tissue mast cells) of Wistar rats and CBA mice was investigated. It was determined that prodigiosan and leukinferon caused degranulation and decreasing in the number of mucosal mast cells, while interferon had no such effect. Prodigiosan decreases histamine and serotonin contents in the peritoneal mast cells, whereas leukinferon decreases histamine content only.

[Functional significance of 2 pathways of energy transport in cardiomyocytes].

To study the relative roles of creatine kinase (CK) and adenylate systems in cardiac energy turnover, the effect of CK inhibitor, iodoacetamide- (IAA, 0.5 mM), and 2-deoxyglucose-(DOG, 2 mM) induced) 65% depletion of adenine nucleotides at slightly decreased CK flux was determined in isolated rat heart. Both substances did not substantially affect contractile parameters of the isovolumic heart.

[Metabolic and functional consequences of complete inhibition of creatine kinase by iodoacetamide in the perfused heart].

Treatment of perfused rat hearts with 0.5 mM iodoacetamide (IAAm) for 15 min at different workloads resulting in a nearly complete inhibition of creatine kinase (CK, 99%) was followed by a rapid decline of the phosphocreatine (PCr) level (30%) and a 2-fold increase of the P(i) level which then stabilized. Conversely, the ATP content started to drop monotonously at the beginning of the IAAm washout and reached 30% 90 min after the IAAm removal under medium load.

[The hemodynamics and activity of the sympathetic-adrenal system in air traffic controllers during their professional duties].

A functional status of the circulation and catecholamines excretion in air traffic controllers operating in two shifts (morning and daytime duties) and attending a Start air traffic control system have been assessed. It is shown that a correlations pattern between parameters under study is significantly altered depending on an occupational activity time. The dependence of hemodynamic shifts on an activity of hormonal and mediator components of sympathetic-adrenal system, an accomplishment of the catecholamine effects on cardiovascular activity with an involvement of alpha- and beta-adrenoreceptor system are shown.

[Adrenergic stimulation of the heart during inhibition of phosphocreatine or adenylate pathways of energy transfer in cardiomyocytes].

Functional and metabolic response of an isovolumically perfused heart of a rat to isoproterenol (0.1 microM) has been studied. A heart with the normal content of adenine nucleotides (AN) and phosphocreatine (PCr) as well as that with the 5-fold reduced AN content (with 2-deoxyglucose treatment) significantly increased cardiac work index (PRP), maximal contraction rate (MCR) and maximal relaxation rate (MRR) (by 50, 30-40 and 100-150%, respectively).

[Role of creatine phosphokinase in the energy supply of the pumping function of the heart].

The cardiac output of isolated working rat heart and left ventricular pressure were estimated in either almost complete inhibition of creatine kinase by iodoacetamide or predominant fall in adenine nucleotides (AdN) content induced by 2-deoxyglucose treatment. In the former case, a profound cardiac pump failure was observed despite almost normal levels of myocardial AdN and phosphocreatine. Those hearts could not maintain the aortic output at standard load due to lower LV systolic pressure, that was accompanied by increased minimal and maximal diastolic pressures by 5-7 mm Hg as well as by LV diastolic stiffness.

[The pavement lining of the vessels in the earthworm (Lumbricus terrestris)].

Effect of some preparatory methods to make ready earthworm tissues for electron microscopical investigation has been studied in order to reveal structural components of the vascular wall. The method for an immediate fixation of the earthworm tissues in liquid nitrogen with a subsequent additional fixation in cooled 2.5% glutar aldehyde, applied in combination with OsO4-thiocarbohydroside-OsO4 method is optimal.

[Structure and function of lymphatic postcapillaries (coordinating mechanism of the processes of interstitial transport and lymphatic resorption].

Investigation performed on topography, structure and resorptive function of lymphatic microvessels in the serous membranes gives possibilities to work out a model on formation and transport of lymph. Lymphatic postcapillaries (LP) - thin-walled endothelial channels with valves make an important link of the lymphatic system. The LP structure is similar to that of capillaries.

[Various subjective and objective indicators of intellectual and emotional stress in air traffic controllers during different work shifts].

The purpose of the study was to examine air-traffic controllers attending the automated air-traffic management system called Start. The data revealed that during the night shift air-traffic controllers experienced significant changes in body functioning in comparison with morning and day shifts (deterioration of self-appraisal indices and elevation of catecholamin excretion). The correlation analysis demonstrated that activation of sympathoardrenal chains (primarily of the mediator chain) had a negative influence on the indices of health state, activity and attitude of mind of air-traffic controllers.

[Biomechanics of the spiral arrangement of muscle elements in the vessels and mechanism of its regulation during hemodynamics].

On the basis of a previously proposed hypothesis about a spiral orientation of muscle elements in the walls of muscular arteries, terminal arteries and arterioles, as a universal regularity, concrete mechanisms of vascular biomechanics and bioregulation at hemodynamics are analyzed. The latter come to a cyclic change of the vasodilator phase into the vasoconstrictory one and vice versa, accompanying with a change of pressures in the local areas of the vessel from the lower to the higher and vice versa along the vascular length. Movement of the pulsed wave with the muscle elements is ensured by a consecutive change of pressures in successive local areas.

[Ultrastructural organization of vessels of the slug (Agriolimax agrestis)].

Ultrastructural analysis on organization of the slug microvessel walls (arterioles, capillaries and venules) has been performed. Transport canals of endothelial cells in the capillaries and venules have been estimated. A conclusion is made that in the evolutionary process of development the vascular endothelium becomes more specialized.

[Structural characteristics of the mediastinal pleura and its blood vessels].

By means of impregnation of the microcirculatory bed vessels after V. V. Kupriyanov and transmissive electron microscopy, topological picture of hemomicrovessels has been estimated in the glomerular constructions of the dog mediastinal pleura.

[Morphofunctional characteristics of the vascular endothelium in the common leech (Clepsina complanta)].

The electron microscopic investigation has revealed in the body of the leech two types of vessels, that together with common signs possess a number of peculiarities in composition of endothelial cells. These vessels have different level of organization: the vessels of the first type, concerning the parenchyma of the leech, are nutritive, and the vessels of the second type combine capacitance and transport functions and are the place of hemolymph renovation at the expense of an active metabolic and synthetic activity of the endothelial cells. A fact is stated concerning a specific position of mitochondria in the endotheliocytes of the second type vessels: from the vessel's lumen they are not covered with plasmolemma along their whole extent and come into an immediate contact with hemolymph.

[Angiogenic effect of colchicine].

The effect of colchicine on the microcirculatory vessels of eye perilimbal area was revealed in 2 groups of experimental rabbits. Colchicine was found to promote the growth of capillaries both during intravenous injections of colchicine solution and during local colchicine application as an ointment. The proper substance of cornea was also changed under the influence of colchicine.