A real-time PCR for rapid identification of Salmonella enterica Gaminara serovar.

Salmonella is one of the leading causes of foodborne illnesses in the USA. When a Salmonella outbreak occurs, rapid identification of the causative serovar is important for tracing the source of contamination and for preventing the further spread of the illness. Each serovar is characterized by the presence of a group-specific somatic O-antigen(s) and an assortment of flagellar phase-1 and phase-2 antigens.

Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples.

The methods currently used for detecting in environmental samples require 2 days to produce results and have limited sensitivity. Here, we describe the development and validation of a real-time PCR screening method that produces results in 18 to 24 h. Primers and probes specific to the gene , group D, and serovar Enteritidis organisms were designed and evaluated for inclusivity and exclusivity using a panel of 329 isolates representing 126 serovars and 22 non- organisms.