Extensive influence of microsporidian infection on sucrose solution consumption, antioxidant enzyme activity, cell structure, and lifespan of Asian honeybees.

is the original host of ceranae, a widespread fungal parasite that causes bee nosemosis, which severely threatens the health of bee colonies and the sustainable development of the apiculture industry. To evaluate the impact of infection on workers, spores were purified and used to inoculate newly emerged workers to evaluate the effects of infection. This was followed by an in-depth investigation of spore load and host sucrose solution consumption.

ame-miR-5119- axis modulates larval-pupal transition of western honeybee worker.

The miRNA plays a key role in the regulation of hormone signaling in insects. The pathways by which miRNAs affect hormone levels are unclear in the honeybee (), an indispensable pollinator in nature. In this study, ame-miR-5119 was overexpressed and knocked down in larvae by feeding mimics and inhibitors, respectively, and we determined that ame-miR-5119 regulates hormone signaling through the target gene ecdysis triggering hormone (), which affects the larval-pupal transition of workers.

Construction of a Full-Length Transcriptome of Western Honeybee Midgut Tissue and Improved Genome Annotation.

Honeybees are an indispensable pollinator in nature with pivotal ecological, economic, and scientific value. However, a full-length transcriptome for , assembled with the advanced third-generation nanopore sequencing technology, has yet to be reported. Here, nanopore sequencing of the midgut tissues of uninoculated and -inoculated workers was conducted, and the full-length transcriptome was then constructed and annotated based on high-quality long reads.

Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection.

Non-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs in the larval guts of A.

3D Vertically Aligned Microchannel Three-Layer All Ceramic Lithium Ion Battery for High-Rate and Long-Cycle Electrochemical Energy Storage.

Due to the growing energy and safety demands, rechargeable all-solid-state Li batteries using metallic Li anode and ceramic-based electrolytes have attracted extensive attentions. However, the inherent safety problem of Li metal anode, the ceramic-electrode low Li conductivity, and the high electrolyte/electrode solid-solid interfacial impedance slow the development of high-performance all-solid-state batteries. In this work, a three-layer all ceramic battery with Li Ti O ceramic as anode, LiCoO as cathode, and Li La TiO as electrolyte to solve the safety problem is proposed.