Mechanical method of inoculating plates for antibiotic sensitivity testing.

A mechanical method of inoculating culture plates for antibiotic sensitivity testing is described. This method, which involves the use of a modified laboratory centrifuge, is rapid and provides a homogeneous distribution of organisms.

Use of sealed agar plates for bacterial cultures under jungle conditions.

Commercially available sealed blood-agar plates have been demonstrated to retain their usefulness for as long as 3 months under jungle conditions without refrigeration.

Routine identification of yeasts with the aid of molybdate-agar medium.

A large number of yeasts, including a variety of species other than Candida albicans, were isolated from clinical specimens. C. tropicalis and Torulopsis glabrata were each found one-third as frequently as C.

Biochemical differentiation of the Enterobacteriaceae with the aid of lysine-iron-agar.

A procedure is described for identifying members of the family Enterobacteriaceae isolated from clinical specimens. The methods are based on primary differentiation of the various groups of bacteria by the use of Kligler Iron Agar and lysine-iron-agar. For identification of Salmonella, Shigella, and Arizona group organisms from stools, Triple Sugar Iron Agar and lysine-iron-agar are employed.