Antifungal lipopeptides from the marine Bacillus amyloliquefaciens HY2-1: A potential biocontrol agent exhibiting in vitro and in vivo antagonistic activities against Penicillium digitatum.

This study aimed to clarify the antifungal activity and action mechanism of the lipopeptides from a marine Bacillus amyloliquefaciens HY2-1 against Penicillium digitatum both in vitro and in vivo. Results showed that HY2-1 lipopeptides exerted obvious inhibitions on spore germination and mycelium growth of P. digitatum.

Dietary Influence on Growth, Physicochemical Stability, and Antimicrobial Mechanisms of Antimicrobial Peptides in Black Soldier Fly Larvae.

Safe antibiotic substitutes are needed given the rise in antimicrobial resistance, environmental contamination, and stringent antibiotic regulations. Insect-derived antimicrobial peptides (AMPs) are promising candidates due to their antimicrobial activity, stability, and safety. This study investigates the antimicrobial mechanism of crude AMP extracts and their physicochemical characteristics in black soldier fly larvae (BSFL).

Preparation of chitosan/cellulose nanofibril composite aerogel and its adsorption performance for Cu(II)-MO binary pollutant.

Heavy metals and organic dyes commonly coexist in water, which pose a serious threat to human health. Herein, a functional aerogel for adsorption of Cu(II)-methyl orange binary-polluted system was prepared. Cellulose nanofibril (CNF) was prepared by 2,2,6,6-tetramethylpiperidinyloxy (TEMPO)-NaBr-NaClO system using abandoned pineapple leaves as the main raw material, and chitosan/cellulose nanofibril (CS/CNF) composite aerogel was constructed by sol-gel method combined with freeze-drying.

Polysaccharides from pineapple peel: Structural characterization, film-forming properties and its effect on strawberry preservation.

The growing demand for food safety has stimulated the development of new environmentally friendly food packaging. It is the development trend of food packaging in recent years by using natural polysaccharides as carriers and adding bioactive ingredients extracted from plants to prepare multifunctional films with antioxidant, antimicrobial and biodegradable properties. Herein, three polysaccharide components (PPE40, PPE60, and PPE80) from pineapple peel were extracted by ultrasound-assisted hot water extraction combined with gradient ethanol precipitation method, which all showed a certain scavenging activities against DPPH, ABTS, and hydroxyl radical.

L-arabinose isomerase from Lactobacillus fermentum C6: Enzymatic characteristics and its recombinant Bacillus subtilis whole cells achieving a significantly increased production of D-tagatose.

L-arabinose isomerase (L-AI) is a functional enzyme for the isomerizing of D-galactose to produce D-tagatose. In this study, L-AI-C6-encoding gene from the probiotic Lactobacillus fermentum C6 was cloned and expressed in Bacillus subtilis WB600 for investigating enzymatic characteristics and bioconverting D-tagatose by means of whole-cell catalysis. Results showed that the engineered B.

An efficient preparation process of sisal fibers via the specialized retting microorganisms: Based on the ideal combination of degumming-related enzymes for the effective removal of non-cellulosic macromolecules.

Bioaugmentation retting with the specialized pectinolytic and xylanolytic microorganisms can accelerate the removal of non-cellulosic macromolecules around plant fibers, thus shortening retting time and facilitating fiber quality. Currently, few specialized microorganisms have been explored for the retting of sisal fibers. The present study excavated the retting fungi including Aspergillus micronesiensis HD 3-6, Penicillium citrinum HD 3-12-3, and Cladosporium sp.

Chitin Deacetylase from TCI-16: Heterologous Expression, Characterization, and Deacetylation Performance.

Chitin deacetylase (CDA) removes the acetyl group from the chitin molecule to generate chitosan in a uniform, high-quality deacetylation pattern. Herein, BaCDA was a novel CDA discovered from our previously isolated strain TCI-16, which was excavated from mangrove soil. The gene was cloned and overexpressed in BL21 (DE3) to facilitate its subsequent purification.

Pretreatment of shrimp shells with an acidic deep eutectic solvent system for chitin extraction and its enhanced performance as a carrier for immobilized lipase.

Existing methods for chitin extraction usually produce substantial waste, which poses ecological hazards. Natural deep eutectic solvent (NADES) offers a promising one-step pretreatment alternative, replacing the resource-intensive demineralization (DM) and deproteinization (DP) process. Hence, in this study, the influence of various acidic NADES, on achieving a simplified one-step DM and DP in the chitin extraction process was investigated.

Co-production of surfactin and fengycin by BBW1542 isolated from marine sediment: a promising biocontrol agent against foodborne pathogens.

Unlabelled: Pathogenic bacteria contaminations and related diseases in food industries is an urgent issue to solve. The present study aimed to explore natural food biopreservatives from microorganisms. Using dilution-plate method, a strain BBW1542 with antimicrobial activities against various foodborne pathogenic bacteria was isolated from the seabed silt of Beibu Gulf, which was identified as by the morphological observation and 16S rDNA sequences.

Structural and enzymatic characterization of a novel metallo-serine keratinase KerJY-23.

KerJY-23 was a novel keratinase from feather-degrading Ectobacillus sp. JY-23, but its enzymatic characterization and structure are still unclear. In this study, the KerJY-23 was obtained by heterologous expression in Escherichia coli BL21(DE3), and enzymatic properties indicated that KerJY-23 was optimal at 60 °C and pH 9.

Effective biodegradation on chicken feather by the recombinant KerJY-23 Bacillus subtilis WB600: A synergistic process coupled by disulfide reductase and keratinase.

Keratin wastes are abundantly available but rich in hard-degrading fibrous proteins, and the keratinase-producing microorganisms have gained significant attention due to their biodegradation ability against keratinous materials. In order to improve the degradation efficiency of feather keratins, the keratinase gene (kerJY-23) from our previously isolated feather-degrading Ectobacillus sp. JY-23 was overexpressed in Bacillus subtilis WB600 strain.

Isolation of a novel feather-degrading Ectobacillus sp. JY-23 strain and characterization of a new keratinase in the M4 metalloprotease family.

Microbial keratinases have prominent potential in biotransformation of recalcitrant keratin substrates to value-added products which has made keratinases a research focus in the past decades. In this study, an efficient feather-degrading bacterium was isolated and identified as a novel species in Ectobacillus genus and designated as Ectobacillus sp. JY-23.

A marine lipopeptides-producing Bacillus amyloliquefaciens HY2-1 with a broad-spectrum antifungal and antibacterial activity and its fermentation kinetics study.

The antagonistic Bacillus amyloliquefaciens HY2-1 was a marine microbiology that was isolated previously from the seabed silt of Beibu Gulf in China by dual culture with Penicillium digitatum. As a continuous study, the present work focused on evaluating the antimicrobial activity, identifying the produced active components, and revealing the fermentation characteristics of B. amyloliquefaciens HY2-1, respectively.

Nitrogen Removal Characteristics of a Marine Denitrifying Pseudomonas stutzeri BBW831 and a Simplified Strategy for Improving the Denitrification Performance Under Stressful Conditions.

A marine aerobic denitrifying bacterium was isolated and identified as Pseudomonas stutzeri BBW831 from the seabed silt of Beibu Gulf in China. According to the genome analysis, P. stutzeri BBW831 possessed a total of 14 genes (narG, narH, narI, narJ, napA, napB, nirB, nirD, nirS, norB, norC, norD, norQ, and nosZ) responsible for fully functional enzymes (nitrate reductase, nitrite reductase, nitric oxide reductase, and nitrous oxide reductase) involved in the complete aerobic denitrification pathway, suggesting that it had the potential for reducing nitrate to the final N.

Liquid hot water pretreatment combined with high-solids enzymatic hydrolysis and fed-batch fermentation for succinic acid sustainable processed from sugarcane bagasse.

In order to sustainable process of bio-succinic acid (SA), response surface methodology (RSM) was applied to optimize liquid hot water pretreatment pretreatment of sugarcane bagasse (SCB), followed by high-solids enzymatic hydrolysis of pretreated residual that without washing, then the hydrolysates and partial pretreatment liquid were used as carbon sources for SA fermentation. Results showed that the highest sugars yield could be achieved at pretreatment conditions of temperature 186 °C, time 25 min and solid-to-liquid ratio 0.08; enzymatic digestion the pretreated residuals at 20 % (w/v) solid content via enzymes reconstruction and fed-batch strategy, the obtained sugars reached to 121 g/L; by controlling the nutrition and conditions of the fermentation process, most of the C5 and C6 sugars in the hydrolysate and pretreatment liquid were converted into SA with a conversion rate high to 280 mg/g SCB.

Exopolysaccharides from Lactobacillus plantarum reduces cadmium uptake and mitigates cadmium toxicity in rice seedlings.

Exopolysaccharides (EPSs) can be used as effective exogenous substances to alleviate the toxic effect of cadmium (Cd) on rice and other crops, thus improving plant growth characteristics under stress conditions, and reducing the accumulation of Cd in grains, but the underlying mechanism is still unclear. In the present work, the effects of EPSs from Lactobacillus plantarum on the efficiency of Cd absorption and distribution in rice seedlings under Cd stress were investigated. The results revealed that growth of rice seedlings was severely inhibited by exposure to Cd, resulting in the decrease of plant height, leaf length and biomass.

Root cell wall remodeling: A way for exopolysaccharides to mitigate cadmium toxicity in rice seedling.

Exopolysaccharides (EPS) are macromolecules with environment beneficial properties. Currently, numerous studies focus on the absorption of heavy metals by EPS, but less attention has been paid to the effects of EPS on the plants. This study explored the effects of EPS from Lactobacillus plantarum LPC-1 on the structure and function of cell walls in rice seedling roots under cadmium (Cd) stress.

Isolation, characterization, and genome sequencing of a novel chitin deacetylase producing TCI-16.

Chitin deacetylase (CDA) is a chitin degradation enzyme that catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues, playing an important role in the high-value utilization of waste chitin. The shells of shrimp and crab are rich in chitin, and mangroves are usually recognized as an active habitat to shrimp and crab. In the present study, a CDA-producing bacterium, strain TCI-16, was isolated and screened from the mangrove soil.

Dynamics of the Bacterial Community's Soil During the In-Situ Degradation Process of Waste Chicken Feathers.

Unlabelled: Bacteria play an important role in the biodegradation of feather waste. The exploration of the related microbial community structure and diversity is essential to improve the performance of feather waste treatment processes. In the present work, an in-situ soil sampled from a poultry farm was directly used to simulate and accelerate the natural degradation processes of feather waste under laboratory conditions, in which the dynamics of the microbial communities was further analyzed by Illumina HiSeq high-throughput 16S rRNA gene sequencing.

Alkaline hydrogen peroxide pretreatment combined with bio-additives to boost high-solids enzymatic hydrolysis of sugarcane bagasse for succinic acid processing.

Alkaline hydrogen peroxide (AHP) pretreatment of sugarcane bagasse (SCB) at mild conditions was optimized with response surface methodology (RSM), then enzymatic hydrolysis was performed at high-solids substrate loading (30 %, w/v), followed by fed-batch fermentation to convert the fermentable sugars into succinic acid (SA). Results showed the AHP pretreatment conditions of HO concentration 5.5 % (v/v), solid-to-liquid ratio 0.

Revealing the Promoting Effect of Betaine on Vitamin B Biosynthetic Pathway of Pseudomonas denitrificans by Using a Proteomics Analysis.

Background: Our previous comparative metabolomics research revealed that betaine (N,N,Ntrimethylglycine, a typically essential methyl-group donor for vitamin B biosynthesis) had powerful promoting effect on the generation of vitamin B precursors and intermediates in vitamin B-producing Pseudomonas denitrificans. However, the integral effect of betaine on the vitamin B12 biosynthetic pathway is still unclear.

Objectives: Considering the vitamin B biosynthetic pathway of P.

Antifungal Action of Antifungalmycin N2 Against Rhizoctonia solani by Disrupting Cell Membrane and Inhibiting Succinate Dehydrogenase.

Antifungalmycin N2 (3-methyl-3,5-amino-4-vinyl-2-pyrone, CHON) was a novel structural antifungal metabolite produced by Streptomyces sp. strain N2. Our previous study reported that the antagonistic interaction between antifungalmycin N2 and Rhizoctonia solani was accompanied by an oxidative stress in R.

Structural characterization and induced copper stress resistance in rice of exopolysaccharides from Lactobacillus plantarum LPC-1.

Excessive accumulation of copper could decrease growth and quality of crops, and little information was currently available on the role of exopolysaccharides (EPS) from Lactobacillus plantarum in inducing copper stress resistance in plants. The main objective of this work was to purify and characterize the EPS produced by our isolated L. plantarum LPC-1, and evaluate its potential protection for rice against copper stress.

Antagonistic activity and mechanism of an isolated Streptomyces corchorusii stain AUH-1 against phytopathogenic fungi.

The various diseases that occur during the growth of plants usually cause a significant reduction in production and quality of agricultural products. Actinomycetes, especially Streptomyces spp., become a valuable biological control resource due to their preponderant abilities to produce various secondary metabolites with novel structure and remarkable biological activity.