[The localization and preliminary characteristics of antigenic sites (B epitopes) in the nucleocapsid protein of the Lassa virus].

Nucleocapsid protein of Lassa virus contains 4 epitope sites. Three of them were localized in amino acid position 123-1 27, 337-346, and 518-527. Monoclonal antibody 1108 specific to Lassa virus NP-protein reacted with 123-127 synthetic peptide in solid-phase ELISA and precipitated nucleocapsid proteins of Machupo, Tacaribe, and Mopeia arenaviruses in RIP.

[Monoclonal antibodies to the Machupo virus: their isolation and preliminary characteristics].

Six monoclonal antibody-producing hybridoma cell lines were generated by fusion of NS-1 myeloma cells with BALB/c immune splenocytes. Monoclonal antibodies (MCA) specific to Machupo virus NP protein were used to study cross-reactivity between pathogenic and nonpathogenic arenaviruses. It was shown that 3140 MCA cross-reacted in IFA with Lassa, Tacaribe, and Tamiami arenaviruses whereas 3101 MCA reacted with Machupo virus alone.

[The production of antibody-producing hybridomas to the Lassa virus].

The work deals with obtaining hybrid cell lines producing monoclonal antibodies to Lassa arenavirus. To obtain preparations for the screening of hybridomas by indirect immunofluorescence techniques, the dynamics of the accumulation of Lassa virus antigen in cell cultures Vero and 4647 was studied. The maximum accumulation of the virus antigen in Vero cells was shown to occur on day 3 after inoculation with a dose of 1.

[Recombinant monoclonal antibodies to the Lassa virus: the formation of reactive paratopes].

Eighteen hybrid lines secreting recombinant monoclonal antibodies to Lassa virus were produced by fusion of mouse splenocytes with antibody-secreting X-63 myeloma cells. Interrelations between the structure and reactivity of the antibodies were studied by different serological and immunochemical methods. Monoclonal antibodies were divided into different groups according to their serological properties and macromolecular structure.

[The isolation and characteristics of recombinant monoclonal antibodies to the Lassa virus].

Recombinant monoclonal antibodies to Lassa virus were produced. The reactivity of the monoclonal antibodies was studied by indirect fluorescence antibody (IFA) technique, enzyme-linked immunosorbent assay (ELISA), and radioimmunoprecipitation method. The observed reactivity did not correlate with IgG isotyping groups.

[Production of infectious virus and the accumulation in Vero cells of Pichinde virus antigens detectable by an indirect fluorescent antibody method].

The dynamics of accumulation of infectious Pichinde virus in the culture medium and virus-specific antigens in cells was studied in relation to multiplicity of infection in a multicycle experiment. Differences in the fluorescence pattern of Pichinde virus antigens in IFAT were found to depend on the use of acetone or formaldehyde for fixation of the infected cells.