Heterologous Biosynthesis of Spinosad: An Omics-Guided Large Polyketide Synthase Gene Cluster Reconstitution in Streptomyces.

With the advent of the genomics era, heterologous gene expression has been used extensively as a means of accessing natural products (NPs) from environmental DNA samples. However, the heterologous production of NPs often has very low efficiency or is unable to produce targeted NPs. Moreover, due to the complicated transcriptional and metabolic regulation of NP biosynthesis in native producers, especially in the cases of genome mining, it is also difficult to rationally and systematically engineer synthetic pathways to improved NPs biosynthetic efficiency.

Development of sp. FR-008 as an emerging chassis.

Microbial-derived natural products are important in both the pharmaceutical industry and academic research. As the metabolic potential of original producer especially is often limited by slow growth rate, complicated cultivation profile, and unfeasible genetic manipulation, so exploring a as a super industrial chassis is valuable and urgent. sp.