Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes.

PCR-based assays for detecting enterohemorrhagic Escherichia coli serogroups O26 and O113 were developed by targeting the wzx (O-antigen flippase) and the wzy (O-antigen polymerase) genes found in the O-antigen gene cluster of each organism. The PCR assays were specific for the respective serogroups, as there was no amplification of DNA from non-O26 and non-O113 E. coli serogroups or from other bacterial genera tested.

[Dislocation of an endoprosthesis of the hip joint operated by the open method.].

The authors evaluated in a series of endoprostheses of the hip joint the X-ray documentation and peroperative revision findings in 8 patients who from a total of 16 dislocations (2.2%) were subjected to surgical revision. Conditions of a postdysplastic hip joint with previous surgery predominated (62%).

Differences in saccadic eye movement-related potentials under regular and irregular intervals of visual stimulation.

The saccadic eye movement-related potentials (SEMRPs) were recorded over various brain areas in a group of righthanders while they performed saccades to visual stimuli appearing either at regular or at irregular time intervals. The premotion positivity, motion execution component and lambda responses were of shorter latencies and lower amplitudes over the parietal areas as compared to the occipital ones. This finding did not depend on the regularity of intervals.

[Computer analysis of muscle reflex responses to electric stimulation of peripheral nerves].

A computer program is described which allows analysis of reflex muscle responses to electric stimulation of peripheral nerves. Basically, the program is derived from an older methodic approach, yet the process of evaluating the obtained means of reflex muscle responses is in this computer assisted procedure simpler and of a higher quality. The proposed improvement of the original methodic approach provides an important advantage for clinical practice in significantly speeding up the investigation and evaluation of reflex muscle responses on using financially accessible computer technique of Czechoslovak make.

[Physiologic range of stabilometry values obtained in the upright posture using a computer].

The authors present physiological ranges of values of seven parameters of stabilometric examinations of the upright posture in man. In order to make the examination effective, the authors suggested and tested three situations which make it possible to characterize the activity of the visual, vestibular and proprioceptive feedback in the process of maintenance of the upright posture. For measuring the supporting forces during the upright posture the authors used a stabilometer with automatic compensation of the body weight.

[Use of Septopal in the treatment of bone infections].

The authors evaluate a group of 29 patients where they used during treatment of a bone infection gentamicin pellets--Septopal Merck. The mean age of the patients was 46.6 years (9-75 years) the mean duration of the symptoms of inflammation before operation was 20 months.

Activity changes in nucleus reticularis gigantocellularis in relation to skilled forelimb movement in rats.

Adult hooded rats were trained to reach for food pellets into a narrow plexiglass tube and the movement of the preferred forelimb was photo-electrically detected. Multiple unit activity (MUA) or single unit activity in n. reticularis gigantocellularis (NGC) was recorded during reaching.

Ensemble reactions of neurons in the reticular formation of the cat--categorization of inputs and temporal influences.

A method of recording ensemble reactions of reticular neurons was applied in anesthetized cats to obtain information about categorization of inputs and the influence of the time factor upon a pattern of responses, reactions to stimulus intensity changes and to complex stimuli, and temporal influences with their relation to plastic changes induced by iterative stimulation. In spite of some variability of the pattern of responses evoked in the reticular formation (RF) by electrical stimulation of the same source, reactions of the parenchyma of the RF differentiate heterotopic stimuli; reactions evoked from receptor areas situated closely to each other exhibited a similar pattern whereas less resemblance was found in the case of responses evoked from sources with a heterosegmental projection and from contralateral sources. Stimulus intensity changes did not influence substantially the pattern of recordings in the RF.

Reticulospinal regulative commands in the cat evoked at the segmental level: the effect of repetitive stimuli.

The reticular formation of the brain stem participates in the neuronal activity which is evoked by an unexpected or intense stimulus and results in a generalized (startle) reaction of the organism. If this reaction is evoked from the segmental receptor level, it comprises a spino-reticulo-spinal (i.e.

Heterotopic stimuli-related potential gradients in a small volume of the medullary reticular formation in the cat.

In anaesthetized cats the role of the medullary reticular formation in discriminative functions was studied. We analysed (1). variability of collective reactions of the reticular neurons (extracellular potential gradients recorded bipolarly in three directions by a tetrad of micropipettes with closely-positioned tips in a small volume of the reticular formation) to the same stimulus, and (2).