[Isolation, culture and cryopreservation of cells derived from fetal appendages].

Objective: To investigate the optimal method for isolation, culture and cryopreservation of cells from fetal appendages, for the purpose of providing viable cells for tissue engineering, cell therapy and gene therapy.

Methods: Trypsin dispersion method was used to isolate cells from human umbilical cord and placenta. The tissues from umbilical cord and placenta were cryopreserved with dimethylsulfoxide (DMSO) in different concentrations.

[Changes in the calcitonin content after inhalation injury in dogs].

Objective: To investigate the changes in the serum content of immunoreactive calcitonin (iCT) after burns or inhalation injury, and to explore its diagnostic significance.

Methods: Twenty-four dogs were randomized into 4 groups, i. e.

An experimental model of an electrical injury to the peripheral nerve.

Objective: Injury to the peripheral nerves is a common complication found in patients suffering from electrical burns. At present, there are many kinds of experimental models for electrical injury, but no report describes an animal-based experimental model for a relatively simple electrical injury to the peripheral nerves. We have designed and constructed a specific device to generate increasingly severe electrical shocks of a known voltage for the experiment.