Infectious Bursal Disease Virus Structural Protein VP2 Expressed by a Baculovirus Recombinant in Bombyx mori.

VP2 cDNA gene of the infectious bursal disease virus HZ96 strain, encoding a major host-protective antigen, was cloned into baculovirus transfer vector pBacPAK8, resulting in a recombinant transfer vector pBacPAK-VP2. The vector pBacPAK-VP2 and linearized DNA of modified baculovirus Bm-BacPAK6 were co-transfected into the cultured Bombyx mori (Bm) N cells, in which homologous recombination occurred. Then, baculovirus recombinants were screened out.

Amplification and Cloning by Long RT-PCR of Full-length Genome of Larger Segment of Chicken Infectious Bursal Disease Virus.

To develop the genetic rescue techniques for infectious bursal disease virus (IBDV), Birnaviridae family, the full-length cDNA of the larger segment of the chicken IBDV was amplified and cloned by long RT-PCR. A comparison of four purification and extraction methods of RNA from IBDV infected chicken embryoid fibroblasts (CEF) showed that the ultracentrifugation followed by proteinase Kdigestion extracted dsRNA more effectively. Then reverse transcription was carried out at 50 degrees using Superscipt II enzyme, followed by RNase H digestion.

The 30-bp deletion variant: a polymorphism of latent membrane protein 1 prevalent in endemic and non-endemic areas of nasopharyngeal carcinomas in China.

Development of nasopharyngeal carcinoma (NPC) is closely associated with Epstein-Barr virus (EBV) infection. However, NPC occurs with a marked geographic and racial distribution, whereas EBV infection is ubiquitous in the world. This leads to a question whether certain subtypes of EBV have a greater potential to induce cell transformation.