Regeneration of ectomycorrhizal fungal isolates following deep freezer storage.

Mycelial growth and survival ratio of ectomycorrhizal fungi were determined after storage at -70℃ for 1, 3, or 6 mon. Seventeen of 23 ectomycorrhizal fungi did not survive after storage for more than 6 mon, whereas Cenococcum geophilum, Lepista nuda, and some species of Rhizopogon and Suillus did survive.

Enzyme Activity of Cenococcum geophilum Isolates on Enzyme-specific Solid Media.

Enzyme activities of Cenococcum geophilum isolates were examined on enzyme-specific solid media. Deoxyribonuclease, phosphatase, and urease were detected in all isolates, whereas cellulase was not detected in any of the isolates. Variations in enzyme activities of amylase, caseinolysis, gelatinase, lipase, and ribonuclease were observed among isolates.

Variation in Sodium Chloride Resistance of Cenococcum geophilum and Suillus granulatus Isolates in Liquid Culture.

We studied the resistance of Cenococcum geophilum and Suillus granulatus isolates to NaCl during growth under axenic culture conditions. C. geophilum isolates displayed variations in NaCl resistance; mycelial growth of most isolates was inhibited above 200mM.

Ectomycorrhizal fungal communities associated with Pinus thunbergii in the eastern coastal pine forests of Korea.

We investigated the ectomycorrhizal (ECM) fungal colonization status of Pinus thunbergii mature trees and regenerating seedlings varying in age in coastal pine forests on the east coast of Korea. We established one 20 x 20-m plot at each of two study sites at P. thunbergii coastal forests in Samcheok.