Transcriptome dynamics in developing leaves from C and C Flaveria species.

C species have evolved more than 60 times independently from C ancestors. This multiple and parallel evolution of the complex C trait suggests common underlying evolutionary mechanisms, which could be identified by comparative analysis of closely related C and C species. Efficient C function depends on a distinctive leaf anatomy that is characterised by enlarged, chloroplast-rich bundle sheath cells and narrow vein spacing.

Streamlined regulation of chloroplast development in the liverwort Marchantia polymorpha.

Chloroplasts develop from undifferentiated plastids in response to light. In angiosperms, after the perception of light, the Elongated Hypocotyl 5 (HY5) transcription factor initiates photomorphogenesis, and two families of transcription factors known as GOLDEN2-LIKE (GLK) and GATA are considered master regulators of chloroplast development. In addition, the MIR171-targeted SCARECROW-LIKE GRAS transcription factors also impact chlorophyll biosynthesis.

MYB-related transcription factors control chloroplast biogenesis.

Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors. However, glk mutants contain residual chlorophyll, indicating that other proteins must be involved. Here, we identify MYB-related transcription factors as regulators of chloroplast biogenesis in the liverwort Marchantia polymorpha and angiosperm Arabidopsis thaliana.

A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C and C grasses.

Background: It has been proposed that engineering the C photosynthetic pathway into C crops could significantly increase yield. This goal requires an increase in the chloroplast compartment of bundle sheath cells in C species. To facilitate large-scale testing of candidate regulators of chloroplast development in the rice bundle sheath, a simple and robust method to phenotype this tissue in C species is required.

Shedding light on AT1G29480 of -An enigmatic locus restricted to Brassicacean genomes.

A key feature of C Kranz anatomy is the presence of an enlarged, photosynthetically highly active bundle sheath whose cells contain large numbers of chloroplasts. With the aim to identify novel candidate regulators of C bundle sheath development, we performed an activation tagging screen with . The reporter gene used encoded a chloroplast-targeted GFP protein preferentially expressed in the bundle sheath, and the promoter of the C phosphopyruvate carboxylase gene from served as activation tag because of its activity in all chlorenchymatous tissues of .

Insights into the control of metabolism and biomass accumulation in a staple C4 grass.

This article comments on: . 2020. BIOMASS YIELD 1 regulates Sorghum biomass and grain yield via the shikimate pathway.

Reporter-based forward genetic screen to identify bundle sheath anatomy mutants in A. thaliana.

The evolution of C photosynthesis proceeded stepwise with each small step increasing the fitness of the plant. An important pre-condition for the introduction of a functional C cycle is the photosynthetic activation of the C bundle sheath by increasing its volume and organelle number. Therefore, to engineer C photosynthesis into existing C crops, information about genes that control the bundle sheath cell size and organelle content is needed.

Transcriptome Assembly and Comparison of C, C-C, and C Species of Tribe Salsoleae (Chenopodiaceae).

C photosynthesis is a carbon-concentrating mechanism that evolved independently more than 60 times in a wide range of angiosperm lineages. Among other alterations, the evolution of C from ancestral C photosynthesis requires changes in the expression of a vast number of genes. Differential gene expression analyses between closely related C and C species have significantly increased our understanding of C functioning and evolution.

C3 cotyledons are followed by C4 leaves: intra-individual transcriptome analysis of Salsola soda (Chenopodiaceae).

Some species of Salsoleae (Chenopodiaceae) convert from C photosynthesis during the seedling stage to the C pathway in adult leaves. This unique developmental transition of photosynthetic pathways offers the exceptional opportunity to follow the development of the derived C syndrome from the C condition within individual plants, avoiding phylogenetic noise. Here we investigate Salsola soda, a little-studied species from tribe Salsoleae, using an ontogenetic approach.