Multimodal spectroscopy combining time-of-flight-secondary ion mass spectrometry, synchrotron-FT-IR, and synchrotron-UV microspectroscopies on the same tissue section.

Mass spectrometry and spectroscopy-based approaches can provide an overview of the chemical composition of a tissue sample. This opens up the possibility to investigate in depth the subtle biochemical changes associated with pathological tissues. In this study, time-of-flight secondary ion mass spectrometry (TOF-SIMS) and synchrotron-FT-IR and -UV imaging were applied to the same tissue section by using the same sample holder.