This report describes six successful pregnancies (five healthy children from four deliveries and two miscarriages) with SrC1(2) oocyte activation using spermatozoa from nine patients with repeated fertilization failure. Oocytes were artificially activated by SrC1(2) 30 min after intracytoplasmic sperm injection (ICSI). Oocytes were placed in 10 mmol/l of SrC1(2) medium for 1 h, rinsed several times, and then cultured in Universal IVF medium.
View Article and Find Full Text PDFPurpose: The aim of this study was to investigate the feasibility of using frozen-thawed testicular sperm as well as the timing of testicular sperm extraction (TESE) in patients with non-mosaic Klinefelter syndrome.
Methods: Intracytoplasmic sperm injection (ICSI) was performed in six of 17 (35%) patients whose sperm was recovered by TESE. Multiple biopsies of both testes were performed on the day of oocyte retrieval in all but one of the six patients.
Objective: To describe the first case of delivery after the transfer of a single blastocyst derived from a vitrified mature human oocyte.
Design: Case report.
Setting: Private assisted reproduction clinic.