[Effects of thymus tissue transplantation on the survival of mice exposed to a lethal X-Ray dose.].

During the radiation exposure of the body, processes similar to those characteristic of the natural aging of mammals occur. This phenomenon is most often referred to as accelerated aging. By transplanting the thymus tissue, it is possible to achieve a normalization of food and water consumption, a stabilization of weight gain, and a significant increase in the life expectancy of experimental animals, even after their lethal irradiation.

Incoherent optical fluctuation flowmetry for detecting limbs with hemodynamically significant stenoses in patients with type 2 diabetes.

Introduction: The development of new highly accurate, inexpensive and accessible methods for the detection of lower-extremity peripheral artery disease (LE-PAD) in diabetic patients is required. The aim of this study was to evaluate the accuracy of a new incoherent optical fluctuation flowmetry (IOFF) method in detecting legs with hemodynamically significant stenoses compared to ankle brachial index (ABI) and transcutaneous oximetry (TcPO2) in patients with diabetes mellitus (DM).

Materials And Methods: Patients were recruited into 2 groups.

Skin microvascular reactivity in patients with diabetic retinopathy.

Aims: Early detection of microangiopathic complications of diabetes mellitus (DM) is necessary to analyze the patient's condition and prevent disease progression. The study was aimed to investigate the relationship between the presence of retinopathy and decreased reactivity of the microcirculatory bed in patients with diabetes.

Methods: The study involved 130 subjects: healthy volunteers (n = 48), DM patients without retinopathy (n = 53) and with retinopathy (n = 29).

Laparoscopic cyst enucleation is a promising technique for treatment of gastrointestinal tract duplications in children.

Background: Laparoscopic cyst enucleation has its advantages and limitations in treatment of gastrointestinal tract (GIT) duplications. It allows to avoid bowel resection in such locations as ileocecal valve. We introduced laparoscopic cyst enucleation with further bowel plasty using cyst muscle layer and without monopolar coagulation, for cyst dissection we used straight scissors.

Incoherent Optical Fluctuation Flowmetry: A New Method for the Assessment of Foot Perfusion in Patients with Diabetes-Related Lower-Extremity Complications.

(1) Background: To date, there are no studies evaluating the ability of the incoherent optical fluctuation flowmetry (IOFF) method to assess foot tissue perfusion. The aim of this study was to evaluate the correlation between perfusion values measured by IOFF and TcPO2 in patients with diabetes-related lower-extremity complications. (2) Methods: This was an observational, cross-sectional, two-center study.

Skin microcirculation reactivity to local thermal hyperaemia in patients with COVID-19 - A pilot observational study.

Background: Endothelial dysfunction and microvascular disturbances are suggested to play a key role in higher morbidity and worse prognosis in patients with COVID-19 and cardiometabolic diseases.

Objective: Study was aimed to establish relationships between the skin microcirculation parameters and various clinical and laboratory indicators.

Methods: The study included 18 patients with moderate disease according to WHO criteria.

The Influence of Spatial Effects on the Dynamics of Solutions in Keynes' Mathematical Model of the Business Cycle.

We consider the special case of the mathematical model of Keynes' business cycle with the spatial interaction. In this model we assume that macroeconomic factors affect a certain geographical region and economic indicators. The dependence occurs on the spatial (geographical) coordinates in addition to the dependence on the temporal evolution, even if the economic subject is externally homogeneous in space.

[Correlation of cutaneous blood microcirculation parameters with biological age in diabetic patients.].

It has been established that the age together with the number of chronic diseases cause the decrease of the reactivity of the microcirculatory bed. This study aims to evaluate the relationship between cutaneous microcirculation parameters and biological and chronological age of patients with diabetes mellitus. 11 diabetic patients (median age 57 (51; 64) years) were examined in course of this study; biological age was figured by Aging.

A review on transcriptomic and metabolomic responses of plants to nanopollution.

Nanoparticles (NPs) are tiny substances that can exist in the soil with different forms at different concentrations. In general, they present enormous effects on the growth, physiology, and molecular responses in plants. Indeed, they can penetrate the roots, stem, and leaves via different ways like stomata, plasmodesmata, xylem, and phloem and through transporter proteins like aquaporins.

Proteomic and molecular dynamic investigations of PTM-induced structural fluctuations in breast and ovarian cancer.

Post-translational processing leads to conformational changes in protein structure that modulate molecular functions and change the signature of metabolic transformations and immune responses. Some post-translational modifications (PTMs), such as phosphorylation and acetylation, are strongly related to oncogenic processes and malignancy. This study investigated a PTM pattern in patients with gender-specific ovarian or breast cancer.

Methods of Non-Invasive In Vivo Optical Diagnostics in the Assessment of Structural Changes in the Skin Induced by Ultraviolet Exposure in an Experimental Model.

Background: This paper demonstrates the use of optical diagnostic methods to assess the dynamic skin changes observed in acute and chronic exposure to ultraviolet (UV) radiation in vivo.

Methods: Firstly, in order to initiate photoaging (chronic UV exposure), animals ( = 40) were divided into two groups: chronic UV exposure ( = 30), and control ( = 10; without irradiation). Photoaging in animals was induced by chronic repeated exposure to UVA radiation three times per week, for 12 weeks continuously, while the UV dose increased stepwise over the course of the experiment (55 minimal erythema doses (MED) in total).

Convolutional neural network in proteomics and metabolomics for determination of comorbidity between cancer and schizophrenia.

The association between cancer risk and schizophrenia is widely debated. Despite many epidemiological studies, there is still no strong evidence regarding the molecular basis for the comorbidity between these two pathological conditions. The vast majority of assays have been performed using clinical records of schizophrenic patients or those undergoing cancer treatment and monitored for sufficient time to find shared features between the considered conditions.

Reduced microvascular reactivity in patients with diabetic neuropathy.

Background: Neurogenic regulation is involved in the development of microcirculation response to local heating. We suggest that microvascular reactivity can be used to estimate the severity of diabetic polyneuropathy (DPN).

Objective: To evaluate the prospects for using the parameters of skin microvascular reactivity to determine the severity of DPN.

Reactivity of skin microcirculation as a biomarker of cardiovascular events. Pilot study.

Background: The role of microcirculatory disorders is progressively being accepted in the pathogenesis of cardiovascular diseases.

Objective: The purpose of current study is to assess whether we can consider skin microcirculation disorders as a biomarker of cardiovascular events.

Methods: Group 1 consisted of healthy volunteers (n = 31); group 2 (n = 42) consisted of patients with diseases that increase the risk of cardiovascular events; group 3 (n = 39) included patients with the history of cardiovascular events.

Synchronization of Fluctuations in the Interaction of Economies within the Framework of the Keynes's Business Cycle Model.

In this paper, we will study two independent economies in a country (national, regional and urban), where the dynamics of fluctuations in each economy is described by Keynes's mathematical business cycle model. This is an interaction of two economies which include trade and competition. In the resulting system that consists of two independent economic entities, we show that fluctuations can emerge as two possible types of economic indicators (synchronous and antiphase) when the peaks and downturns of business activities in each of the economies are completely synchronized or on the contrary when the rise of one economy is accompanied by a recession (antiphase cycles).

Perspectives on using laser fluorescence spectroscopy in chronological skin ageing assessment.

Introduction: Laser fluorescence spectroscopy (LFS) is a potential tool for diagnosing pathological skin processes such as fibrosis, hypoxia, and inflammation. This article describes the results of the non-invasive assessment of skin autofluorescence for animals of different ages.

Methods: The study was performed on outbred white male mice (n = 14).

Fibroin-Gelatin Composite Stimulates the Regeneration of a Splinted Full-Thickness Skin Wound in Mice.

The effects of composite fibroin-gelatin microparticles (100-250 μ) on the rate of wound healing and regeneration under conditions of contraction prevention were studied on the model of splinted full-thickness skin wound in a mouse. Subcutaneous injection of these particles into the defect area accelerated wound healing and promoted re-epithelialization and recovery of normal structure of the epidermis. In addition, the composite microparticles promoted the formation of connective tissue of characteristic structure, replacing the derma over the entire defect, and stimulated regeneration of subcutaneous muscle (panniculus carnosus) and skin appendages (sebaceous glands and hair follicles).

[Possible approaches to increase longevity of experimental animals.].

A statistically significant difference in the cumulative survival of Wistar rats born in different seasons has been shown: animals born in late November - early December had an average lifespan by 24,8% longer, a minimum lifespan by 14,3% longer, and a maximum lifespan by 20,7% longer in comparison with animals born in April. The geroprotective effect of the technique of thymus tissue transplantation into the immune privileged anterior chamber of the eye in animals born both in November and April has been shown.

Approaches to improve the predictive value of laser Doppler flowmetry in detection of microcirculation disorders in diabetes mellitus.

Background: Laser Doppler flowmetry is widely used in scientific studies of blood microcirculation but constrained in clinical use due to the low diagnostic significance.

Objective: This research is aimed at creation of approaches to prognostic value increase in detection of microcirculation disorders in patients with type 2 diabetes mellitus.

Methods: The study included 10 volunteers and 10 patients with t2DM.

Fluorescence of radiation-induced tissue damage.

Purpose: The aim of this study was evaluating changes in the photosensitizer fluorescence in vivo in the radiation-induced damage area in comparison of intact areas with a simultaneous assessment of changes both in blood parameters and in histological data.

Materials And Methods: The study was conducted in white outbred SHK mice (n = 21). Their right hindlimbs were irradiated with a dose of 25 Gy after the intraperitoneal injections of photosensitizer 'Photosens'.

Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers.

We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the large surface area, and the contribution of microcarrier mineralization to the stimulation of the osteogenic differentiation of mesenchymal stem cells was revealed.

Regeneration of jejunal wall defect using an implant based on silk fibroin fibers.

Regenerative properties of fibroin implant vitalized with allogeneic bone marrow cells were assessed. The study was performed using the experimental model of rat jejunum wall damage. Three weeks after surgery, we observed recovery of all layers of the jejunum wall at the site of injury and complete degradation of the implant material.

[Pathogenetic significance of C774T single nucleotide polymorphism of the endothelial NO synthase gene in the development of metabolic syndrome].

The relationship between nitric oxide production and metabolic disorders and the role of endothelial nitric oxide synthase (eNOS or NOS3) in metabolic syndrome (MS) remain poorly understood and need deeper investigation. In this context the role of the NOS3 gene in pathogenesis of MS is of special interest. The aim of the study was to investigate association of NOS3 single nucleotide polymorphism C774T with risk of MS in the Slavic population of the Kaliningrad region and the relationship of this polymorphic variant with some parameters of endothelial dysfunction.

New Silk Fibroin-Based Bioresorbable Microcarriers.

We fabricated bioresorbable microcarriers from water solution of Bombyx mori silk fi broin. The microcarriers are 3D structures with intricate surface and pores allowing penetration of culture medium, gas exchange, and cell adhesion. Fibroin molecules form hydrophobic structures and normally have a negative charge, which stimulates migration, but inhibits cell adhesion and makes it less effective.

Effects of allogenic fetal pancreatic tissue transplantation on regeneration of islet cells in recipient rats with alloxan-induced diabetes mellitus.

The linear parameters and number of Langerhans islets were evaluated in rats with alloxaninduced diabetes mellitus after transplantation of fetal pancreatic tissue to the anterior chamber of the eye. The islets significantly increased in size by week 3 after surgery and a trend to an increase in their number was observed.