Extracorporeal Photopheresis in Children with Chronic Graft-Versus-Host Disease.

Chronic graft versus host disease (cGVHD) remains a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). It significantly decreases survival and quality of life. The present study demonstrates retrospective data on extracorporeal photopheresis (ECP) in children with cGVHD.

Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II-Wettability and Biological Performance.

This work describes the wettability and biological performance of Zn- and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45°. An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6° with water and to 17° with glycerol.

[Hemostatic Gene Polymorphisms in Acute Coronary Syndrome with Nonobstructive Coronary Atherosclerosis].

Purpose: to study distribution of genes of the coagulation system, unfavorable in relation to the risk of thrombosis, and their influence on serum parameters ofthe hemostasis system in patients with nonobstructive coronary atherosclerosis (NCA) and acute coronary syndrome (ACS).

Materials And Methods: We included in this nonrandomized open study patients with ACS older than 18 years with intact coronary arteries or confirmed at coronary angiography stenosis <50%. Genotypes of these patients were analyzed by 8 polymorphic variants of the hemostatic system genes which previously were found to be associated with the thrombophilia risk: F2 (20210 G>A) rs1799963, F5 (1691 G>A) rs6025, F7 (10976G>A) rs6046, F13 (163 G>T) rs5985, F1 (-455G>A) rs1800790, GP Ia - Ila (807C>t) rs1126643, GP Ilb-IIIa (1565 T>C) rs5918, PAI-I (-6755G>4G) rs1799889.

Electric field control of magnon-induced magnetization dynamics in multiferroics.

We consider theoretically the effect of an inhomogeneous magnetoelectric coupling on the magnon-induced dynamics of a ferromagnet. The magnon-mediated magnetoelectric torque affects both the homogeneous magnetization and magnon-driven domain wall motion. In the domains, we predict a reorientation of the magnetization, controllable by the applied electric field, which is almost an order of magnitude larger than that observed in other physical systems via the same mechanism.

Controlling superconducting spin flow with spin-flip immunity using a single homogeneous ferromagnet.

Spin transport via electrons is typically plagued by Joule heating and short decay lengths due to spin-flip scattering. It is known that dissipationless spin currents can arise when using conventional superconducting contacts, yet this has only been experimentally demonstrated when using intricate magnetically inhomogeneous multilayers, or in extreme cases such as half-metals with interfacial magnetic disorder. Moreover, it is unknown how such spin supercurrents decay in the presence of spin-flip scattering.

Dynamic excitation states and firing patterns are controlled by sodium channel kinetics in myenteric neurons: a simulation study.

Enteric neurons located in the gastro-intestinal tract are of particular importance to control digestive functions such as motility and secretion. In our recent publication, we showed that mouse myenteric neurons exhibit 2 types of tetrodotoxin-resistant Na(+) currents: a fast inactivating Na(+) current produced by Nav1.5 channels, present in nearly all myenteric neurons, and a persistent Na(+) current attributed to Nav1.

[Extracorporeal photopheresis in the treatment of patients with refractory chronic graft-versus-host disease after allogeneic bone marrow transplantation].

Aim: To evaluate the efficiency of extracorporeal photopheresis (ECP) in the treatment of patients with refractory chronic graft-versus-host disease (cGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).

Subjects And Methods: The study included 49 patients aged 2 to 55 years. Allo-HSCT was carried out in 38 (79%) patients with acute leukemias, 5 (10%) with chronic leukemias, 4 (8%) with myelodysplastic syndrome/myeloproliferative disease, and 2 (3%) with other hematologic diseases.

[Effects of strontium ranelate on mononuclear leukocyte culture].

Strontium ranelate (SR) effect on the functional parameters of human blood mononuclear leukocytes culture has been investigated. SR in concentrations of 2.9, 29, and 290 mg/ml produces a dose-dependent decrease in the activity of alkaline phosphatase and the content of calcium and potassium ions in supernatants.

[Dynamical electrical states of heterogeneous populations of ion channels in the membranes of excitable cells].

In computer models, we studied instantaneous (time-varying) current-voltage relationships (iIVs) of populations of ion channels characteristic of the membrane of different type excitable cells, of which the responses to electrical stimuli essentially differ: giant squid axon (Hodgkin-Huxley model), cardiomyocyte, dendrites of CA3 hippocampal pyramidal neurons and Purkinje neurons of the cerebellum. The membrane potential was stepped from the rest level to a certain depolarization test level that was clamped for a certain time, and the total current was measured at different moments after the step onset. For each iIV zero-current points (potentials) were determined.

[Modern possibilities of the pharmacotherapy of acute bronchitis].

The obtained results reflect data of multicenter open prospective randomized comparative study that included 150 patients with acute bronchitis. The estimation of the efficacy of recombinant human interferon 2 in the form of suppositories--genferon and viferon in the treatment of acute bronchitis in adults is given. It was revealed that the usage of these medications in the treatment of acute bronchitis is accompanied by a more dynamic regression of fever and intoxication syndromes, reduces the need for the appointment of additional medications and reduces the period of temporary disability of patients.

The electro-dynamics of the dendritic space in Purkinje cells of the cerebellum.

The functional geometry of the reconstructed dendritic arborization of Purkinje neurons is the object of this work. The combined effects of the local geometry of the dendritic branches and of the membrane mechanisms are computed in passive configuration to obtain the electrotonic structure of the arborization. Steady-currents applied to the soma and expressed as a function of the path distance from the soma form different clusters of profiles in which dendritic branches are similar in voltages and current transfer effectiveness.

Heterogeneous synaptic covering and differential charge transfer sensitivity among the dendrites of a reconstructed abducens motor neurone: correlations between electron microscopic and computer simulation data.

Ultrastructural studies on the synaptology of dendritic arborizations of motoneurones have been problematic because dendrites are very thin in relation to their great length, and most of the studies on this topic have therefore dealt with only small parts of the dendritic tree. Here we compared the ultrastructural characteristics of the axon terminals distributed along the various dendrites of a single motoneurone. For this purpose, the light microscopic 3D reconstruction of the dendritic arborization of an intracellularly labelled abducens motoneurone was combined with an electron microscopic analysis of its synaptic contacts.

Spatial reconfiguration of charge transfer effectiveness in active bistable dendritic arborizations.

The aim of this work was to explore the electrical spatial profile of the dendritic arborization during membrane potential oscillations of a bistable motoneuron. Computational simulations provided the spatial counterparts of the temporal dynamics of bistability and allowed simultaneous depiction the electrical states of any sites in the arborization. We assumed that the dendritic membrane had homogeneously distributed specific electrical properties and was equipped with a cocktail of passive extrasynaptic and NMDA synaptic conductances.

Activity-dependent reconfiguration of the effective dendritic field of motoneurons.

A neuron in vivo receives a continuous bombardment of synaptic inputs that modify the integrative properties of dendritic arborizations by changing the specific membrane resistance (R(m)). To address the mechanisms by which the synaptic background activity transforms the charge transfer effectiveness (T(x)) of a dendritic arborization, the authors simulated a neuron at rest and a highly excited neuron. After in vivo identification of the motoneurons recorded and stained intracellularly, the motoneuron arborizations were reconstructed at high spatial resolution.

[The hygienic assessment of atmospheric pollution by xylene isomers].

The biological action of xylol isomers as ambient air pollutants was investigated. The maximum single allowable concentrations of orthoxylol (0.3 mg/m3), methaxylol (0.

Geometry-induced features of current transfer in neuronal dendrites with tonically activated conductances.

The impact of dendritic geometry on somatopetal transfer of the current generated by steady uniform activation of excitatory synaptic conductance distributed over passive, or active (Hodgkin-Huxley type), dendrites was studied in simulated neurons. Such tonic activation was delivered to the uniform dendrite and to the dendrites with symmetric or asymmetric branching with various ratios of branch diameters. Transfer effectiveness of the dendrites with distributed sources was estimated by the core current increment directly related to the total membrane current per unit path length.

[Status of the pool of hematopoietic stem cells in the peripheral blood in patients with lung cancer before and during polychemotherapy].

The pool of hematopoietic cells-precursors from the peripheral blood of patients with lung cancer stage III-IV was characterized by reduced number of circulating cells-precursors of granulomonocyto-(CFU-GM) and erythropoiesis (CFU-E). In the course of antitumor chemotherapy the counts of CFU-GM and CFU-E diminished 2 and 4-fold, respectively. The prednisolone functional test determined that depletion of CFU-GM and CFU-E pools in the peripheral blood was preceded with lowered pool reserve for hematopoietic precursors.

[Marginal and bone marrow pools of neutrophil granulocytes in patients with lung cancer].

Marginal and bone marrow pools of neutrophil leucocytes were studied in 194 patients with lung cancer, stage III-IV. Reactions of neutrophil marginal pools (adrenaline test) were characterized by the development of neutrophil leucocytosis (NL) in the peripheral blood of 64.5% of cases (typical reaction), or by NL absence in 35.

[The cytochemical characteristics of neutrophil leukocytes in lung cancer before and during antineoplastic chemotherapy].

A number of cytochemical characteristics and the NBT test were studied in neutrophilic granulocytes of 194 patients with diffuse pulmonary carcinoma (Stages III-IV), 31 patients with chronic nonspecific pulmonary diseases, and 20 normal subjects. Changes in the neutrophilic morphology and function were revealed in lung cancer patients, presenting as elevated alkaline phosphatase activity, reduced myeloperoxidase activity and lipid and glycogen levels, increased endogenous activation of the neutrophils in the NBT test, and decreased reaction activity in zymosan stimulation. Antitumor chemotherapy involved a lowering of the cationic protein level, as well of the acid phosphatase activity, and elevation of glycogen content.