Mastoparan induces Ca(2+)-dependent deflagellation of the unicellular green alga Chlamydomonas moewusii Gerloff, as well as the activation of phospholipase C and the production of inositol 1,4, 5-trisphosphate (InsP(3); T. Munnik et al., 1998, Planta 207: 133-145).
View Article and Find Full Text PDFWe previously reported that transcripts for a 30-kD nodulin (Npv30) are very abundant in the nodule. In this paper we describe the isolation and characterization of Npv30 cDNA and genomic clones. Npv30 has the following characteristic features: (a) a putative signal sequence at the deduced amino-terminal region, (b) a proline-rich stretch at the carboxy terminus, and (c) a characteristic domain of four cysteines that resemble metal-binding sites.
View Article and Find Full Text PDFA Phaseolus vulgaris uricase II cDNA clone has been isolated and sequenced. Comparison on the nucleotide level between this clone and the soybean uricase II clone revealed 88.8% identity.
View Article and Find Full Text PDFAlcohols induce mating-structure activation in Chlamydomonas eugametos gametes. From the effect of ethanol on the (32)P-labelling of polyphosphoinositides, we conclude that the synthesis of these lipids is stimulated. Biologically inactive concentrations of ethanol (<6%) had no effect on synthesis, but 6-8% ethanol stimulated synthesis for upto 60 min.
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