Mechanical force promotes tissue and molecular changes in adipose tissue regeneration post-transplantation.

Introduction: Fat grafting often yields inconsistent and suboptimal results, necessitating improved fat processing techniques. A stromal vascular fraction (SVF) gel created using mechanical emulsification demonstrates superior retention rates to conventional Coleman fat grafts.

Methods: This study investigated the mechanisms at play by transplanting fat aspirates from liposuction patients-either processed as Coleman fat grafts or further refined into an SVF gel via mechanical shear force-onto the backs of nude mice.

Dermatoscope for the diagnosis of erythema with purpura induced by lidocaine/prilocaine cream: A case report.

Background: Lidocaine/prilocaine (EMLA) cream is a local anesthetic that is applied to the skin or mucosa during painful therapeutic procedures with few reported side effects.

Case Summary: Here, we report the use of dermatoscopy to identify a case of erythema with purpura, a rare side effect, after the application of 5% EMLA cream.

Conclusion: We conclude that erythema with purpura is caused by irritation and toxicity associated with EMLA, but the specific mechanism by which the toxic substance affects skin blood vessels is unclear.

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat.

The retention of fat-derived grafts remains a challenge for regenerative medicine. Fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare a stromal vascular fraction (SVF)/extracellular matrix (ECM) gel. The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3, 14, 28, and 60 days following transplantation on the backs of nude mice.

Tear trough deformity: different types of anatomy and treatment options.

Aim: To explore the efficacy of tear trough deformity treatment with the use of hyaluronic acid gel or autologous fat for soft tissue augmentation and fat repositioning via arcus marginalis release.

Material And Methods: Seventy-eight patients with the tear trough were divided into three groups. Class I has tear trough without bulging orbital fat or excess of the lower eyelid skin.

Congenital arhinia: A rare case.

Patient: Male, 4 months

Final Diagnosis: Congenital arhynia Symptoms: Absence of the nose Medication: - Clinical Procedure: - Specialty: Pediatrics and Noenatology • Genetics.

Objective: Congenital defects.

Background: Congenital nasal absence (arhinia) is an extremely rare malformation.

Compressed collagen gel as the scaffold for skin engineering.

Collagen gel scaffolds can potentially be utilized as cell seeded systems for skin tissue engineering. However, its dramatic contraction after being mixed with cells and its mechanical weakness are the drawbacks for its application to skin engineering. In this study, a compressed collagen gel scaffold was fabricated through the rapid expulsion of liquid from reconstituted gels by the application of 'plastic compression'(PC) technique.

Skin epithelial cells in mice from umbilical cord blood mesenchymal stem cells.

Unlabelled: The purpose of this study was investigation of the potential to isolate mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) and differentiate them into epithelial cells in mouse skin tissues. Mononuclear cells (MNCs) from UCB (UCB-MNCs) were isolated and induced to MSCs in culture. UCB-MSCs were transfected with pEGFP and labeled with PKH26 dye.

[Construction of a tissue engineering skin with epidermal stem cells].

Objective: To constitute a composite skin substitute that can proliferate well with epidermal stem cells and fibroblasts on collagen sponge.

Methods: Epidermal stem cells were selected by rapid attachment to collagen IV for 10-15 min and cultured on 3T3 feeder layers. Collagen was extracted from rat tail.

[Separation and culture of human epidermal stem cells in vitro].

Objective: To investigate the culture method for epidermal stem cells in vitro.

Methods: The epidermis was separated from the dermis, and shaken for 10 min in 0.05% trypsin at 37 C to dissociate into single cells.

An experimental study on the repair of full skin loss of nude mice with composite graft of epidermal stem cells.

This study is to constitute a composite skin substitute with epidermal stem cells (ESCs) and fibroblasts on collagen sponge. ESCs were selected by rapid attachment to collagen IV for 10 min. Collagen was extracted from rat's tail.