Publications by authors named "Kuhn P"

Background: Physical separation contributes to parental trauma and poor bonding in the context of therapeutic hypothermia (TH) for hypoxic-ischemic encephalopathy (HIE). Parental holding (PH) may improve parents' experience. We aim to determine the physiological and behavioral stability of the newborn held by the parents during TH.

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Aim: Healthcare givers are exposed to stress and therefore are at risk of the development of pathologies. We aimed to provide recommendations regarding psychosocial risks such as stress, moral distress, burnout syndrome or secondary stress syndrome in neonatal care units to best support neonatal healthcare givers.

Methods: We searched PubMed for articles published from 1 January 2017 to 1 December 2023 by using the keywords burnout OR (moral and distress) AND neonatal unit.

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Article Synopsis
  • The research investigates how prostate cancer adapts to changing environments and the role of the Polyaneuploid Cancer Cell (PACC) state in metastasis, suggesting that PACC can enhance metastatic potential.
  • Evidence from previous studies shows that the presence of PACC in patient tumors correlates with a higher risk of future metastasis, prompting the use of innovative methods to analyze circulating and disseminated tumor cells from animal models.
  • The findings reveal that a significant majority of these tumor cells were in the PACC state, and specific traits associated with PACC contribute to its ability to spread and colonize new sites, raising concerns about the effectiveness of certain cancer treatments that may inadvertently promote metastasis.
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Background/objectives: Up to 30% of patients with breast cancers will develop brain or leptomeningeal metastases, and this risk is especially high with HER2-positive cancers. For patients with central nervous system metastases, cerebrospinal fluid (CSF) liquid biopsies are a promising opportunity to monitor disease, inform treatment, and predict prognosis. This pilot study investigated CSF liquid biopsy analytes from three patients diagnosed with central nervous system metastases based on imaging but not confirmed via clinical cytology.

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  • Therapeutic resistance in cancer, especially in advanced prostate cancer, contributes to high mortality as patients often relapse after initial treatments.
  • Research on circulating tumor cells with increased genomic content (CTC-IGC) from 44 advanced prostate cancer patients reveals a link to poorer survival outcomes and clonal origins, indicating polyploidization.
  • Identification of new RNA and protein markers associated with chemotherapy resistance, such as HOMER1, TNFRSF9, and LRP1, suggests pathways for improving cancer treatment and understanding relapse mechanisms.
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Proteome profiling of formalin-fixed paraffin-embedded (FFPE) specimens has gained traction for the analysis of cancer tissue for the discovery of molecular biomarkers. However, reports so far focused on single cancer entities, comprised relatively few cases and did not assess the long-term performance of experimental workflows. In this study, we analyze 1220 tumors from six cancer entities processed over the course of three years.

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Recent advancements in aqueous humor (AH) cell-free DNA (cfDNA) genomics have opened new avenues for ex vivo molecular profiling of retinoblastoma (RB), the most common pediatric intraocular malignancy, where biopsy is typically prohibited. While these insights offer a genetic blueprint of the tumor, they lack multi-omic molecular phenotyping, which is essential for understanding the functional state. Extracellular vesicles (EVs), naturally present in AH, are promising by offering time-resolved phenotypic information.

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Background: Cancer becomes lethal as it spreads from the primary site to the rest of the body. Circulating tumor cells (CTCs) are biomarkers of disease progression and have been associated with decreased overall survival. Blood filtration is a novel concept for removing CTCs from circulation to improve patient prognosis.

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Therapeutic resistance in cancer significantly contributes to mortality, with many patients eventually experiencing recurrence after initial treatment responses. Recent studies have identified therapy-resistant large polyploid cancer cells in patient tissues, particularly in late-stage prostate cancer, linking them to advanced disease and relapse. Here, we analyzed bone marrow aspirates from 44 advanced prostate cancer patients and found the presence of CTC-IGC was significantly associated with poorer progression-free survival.

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This paper aims to present clear and evidence-based proposals for the integration of Early Parental Vocal Contact into the clinical practices of neonatal units. In the first part, we present a comprehensive rationale exploring the ontogenesis of voice perception in both term and preterm newborns that establishes a foundational understanding. This knowledge serves as a crucial starting point for developing evidence-based auditory and multisensory interventions aimed at fostering the developmental trajectory of preterm infants.

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RT027 strains cause infections that vary in severity from asymptomatic to lethal, but the molecular basis for this variability is poorly understood. Through comparative analyses of RT027 clinical isolates, we determined that isolates that exhibit greater variability in their flagellar gene expression exhibit greater virulence . flagellar genes are phase-variably expressed due to the site-specific inversion of the 5'UTR region, which reversibly generates ON vs.

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Background: Lung cancer screening with low-dose computed tomography (CT) scans (LDCT) has reduced mortality for patients with high-risk smoking histories, but it has significant limitations: LDCT screening implementation remains low, high rates of false-positive scans, and current guidelines exclude those without smoking histories. We sought to explore the utility of liquid biopsy (LBx) in early cancer screening and diagnosis of lung cancer.

Methods: Using the high-definition single-cell assay workflow, we analyzed 99 peripheral blood samples from three cohorts: normal donors (NDs) with no known pathology (n = 50), screening CT patients (n = 25) with Lung-RADS score of 1-2, and biopsy (BX) patients (n = 24) with abnormal CT scans requiring tissue biopsy.

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Therapeutic resistance in cancer significantly contributes to mortality, with many patients eventually experiencing recurrence after initial treatment responses. Recent studies have identified therapy-resistant large polyploid cancer cells in patient tissues, particularly in late-stage prostate cancer, linking them to advanced disease and relapse. Here, we analyzed bone marrow aspirates from 44 advanced prostate cancer patients and found the presence of circulating tumor cells with increased genomic content (CTC-IGC) was significantly associated with poorer progression-free survival.

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In the United States, 2.0 million new cancer cases and around 600,000 cancer deaths are estimated to occur in 2024. Early detection gives cancer patients the best chance for treatment success.

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Article Synopsis
  • Researchers are tackling the challenges of using MEK inhibitors in cancer treatment due to off-target toxicity and the need for better predictive markers; they suggest that E-cadherin levels could help predict MEK inhibitor effectiveness.
  • Instead of using traditional methods that require frequent high-dose injections, the study introduces a new approach with a thermosensitive and biodegradable hydrogel that can release MEK inhibitors and doxorubicin locally and sustainably.
  • This innovative hydrogel-liposome system shows promise in reducing tumor growth and improving survival rates in E-cadherin-positive triple-negative breast cancer models, while minimizing toxicity.
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Aging negatively impacts skin health, notably through the senescent cell phenotype, which reduces collagen production and leads to thinner, more fragile skin prone to injuries and chronic wounds. We designed a drug delivery system that addresses these age-related issues using a hybrid hydrogel-nanoparticle system that utilizes a poly(δ-valerolactone--lactide)--poly(ethylene-glycol)--poly(δ-valerolactone--lactide) (PVLA-PEG-PVLA) hydrogel. This hydrogel allows for the local, extended release of therapeutics targeting both proliferating and senescent cells.

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This study explores the critical success factors and barriers to mHealth implementation in South Africa and Tanzania. Through an unstructured literature review and semi-structured interviews with eight mHealth experts, the study uncovers common challenges, including lack of alignment with user needs, inadequate government support, and sustainability issues. Critical success factors identified include user-friendly design and adaptable tools offered at low or no cost.

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Article Synopsis
  • There is a significant need to better understand how prostate cancer spreads, specifically the role of the Polyaneuploid Cancer Cell (PACC) state in this process.
  • Emerging research suggests that the PACC state enhances a cancer cell's ability to metastasize, with a retrospective study indicating that the presence of PACCs in prostate tissue can predict future metastasis.
  • Innovative flow-cytometric techniques reveal that a high percentage (74%) of circulating and disseminated tumor cells are in the PACC state, and further experiments demonstrate that these cells can regain the ability to proliferate at metastatic sites, linking PACCs to increased metastatic potential.
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Background: Heterogeneity can impact biomarker identification. Thus, we investigated the somatic copy number alterations (SCNAs) of individual tumor cells in the vitreous humor of a retinoblastoma patient using single-cell whole-genome profiling and explored the genomic concordance among vitreous and aqueous humor, vitreous seeds, and tumor.

Methods: Aqueous humor (AH), vitreous humor (VH), and tumor biopsy were obtained from an enucleated globe with retinoblastoma and vitreous seeding.

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Article Synopsis
  • Latrodectism, caused by widow spider bites, leads to severe pain and various health complications, posing a significant global health issue, particularly in developing countries.
  • Current treatments include equine serum-derived antivenoms, which have potential risks like allergic reactions, batch variability, and controversial efficacy since latrodectism is seldom fatal.
  • Research has developed fully human antibodies that effectively neutralize alpha-latrotoxin from widow spiders, demonstrating potential for safer, more targeted therapies and diagnostics in the future.
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There is a growing interest in the composition of amniotic fluid (AF) in both humans and animals. In addition to its nutritional and protective functions for the foetus, current knowledge demonstrates that AF also serves advanced diagnostic, prognostic, and therapeutic roles. Newborn dogs have an underdeveloped immune system, making them highly susceptible to dangerous pathogens such as canine parvovirus (CPV-2), canine infectious hepatitis virus (CAdV-1), and canine distemper virus (CDV), thus exposing them to a high risk of mortality in the first weeks of life.

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Despite recent therapeutic advances, metastatic castration-resistant prostate cancer (mCRPC) remains lethal. Chimeric antigen receptor (CAR) T cell therapies have demonstrated durable remissions in hematological malignancies. We report results from a phase 1, first-in-human study of prostate stem cell antigen (PSCA)-directed CAR T cells in men with mCRPC.

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Undifferentiated small round cell sarcomas (USRS) of bone and soft tissue are a group of tumors with heterogenic genomic alterations sharing similar morphology. In the present study, we performed a comparative large-scale proteomic analysis of USRS (n = 42) with diverse genomic translocations including classic Ewing sarcomas with EWSR1::FLI1 fusions (n = 24) or EWSR1::ERG fusions (n = 4), sarcomas with an EWSR1 rearrangement (n = 2), CIC::DUX4 fusion (n = 8), as well as tumors classified as USRS with no genetic data available (n = 4). Proteins extracted from formalin-fixed, paraffin-embedded pretherapeutic biopsies were analyzed qualitatively and quantitatively using shotgun mass spectrometry (MS).

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In the United States, lung cancer is the second most common type of cancer with non-small cell lung cancer (NSCLC) encompassing around 85% of total lung cancer cases. Late-stage patients with metastatic disease have worsening prognosis, highlighting the importance of longitudinal disease monitoring. Liquid biopsy (LBx) represents a way for physicians to non-invasively track tumor analytes, such as circulating tumor cells (CTCs), and understand tumor progression in real-time through analyzing longitudinal blood samples.

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Background And Aims: Parental guidance is essential for supporting parental involvement, maintaining the quality and safety of infant care, and limiting parental stress. The efficiency of a new tool to support parental empowerment - "Step by step with my baby" - was evaluated. The perception of this tool by parents and nurses was studied.

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