Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase.

An indigenous microalga Chlorella vulgaris ESP-31 grown in an outdoor tubular photobioreactor with CO(2) aeration obtained a high oil content of up to 63.2%. The microalgal oil was then converted to biodiesel by enzymatic transesterification using an immobilized lipase originating from Burkholderia sp.

Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31.

The growth and lipid productivity of an isolated microalga Chlorella vulgaris ESP-31 were investigated under different media and cultivation conditions, including phototrophic growth (NaHCO(3) or CO(2), with light), heterotrophic growth (glucose, without light), photoheterotrophic growth (glucose, with light) and mixotrophic growth (glucose and CO(2), with light). C. vulgaris ESP-31 preferred to grow under phototrophic (CO(2)), photoheterotrophic and mixotrophic conditions on nitrogen-rich medium (i.

Nitrogen starvation strategies and photobioreactor design for enhancing lipid content and lipid production of a newly isolated microalga Chlorella vulgaris ESP-31: implications for biofuels.

Microalgae are recognized for serving as a sustainable source for biodiesel production. This study investigated the effect of nitrogen starvation strategies and photobioreactor design on the performance of lipid production and of CO(2) fixation of an indigenous microalga Chlorella vulgaris ESP-31. Comparison of single-stage and two-stage nitrogen starvation strategies shows that single-stage cultivation on basal medium with low initial nitrogen source concentration (i.

Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review.

Microalgae have the ability to mitigate CO(2) emission and produce oil with a high productivity, thereby having the potential for applications in producing the third-generation of biofuels. The key technologies for producing microalgal biofuels include identification of preferable culture conditions for high oil productivity, development of effective and economical microalgae cultivation systems, as well as separation and harvesting of microalgal biomass and oil. This review presents recent advances in microalgal cultivation, photobioreactor design, and harvesting technologies with a focus on microalgal oil (mainly triglycerides) production.

Harvesting of Scenedesmus obliquus FSP-3 using dispersed ozone flotation.

The Scenedesmus obliquus FSP-3, a species with excellent potential for CO(2) capture and lipid production, was harvested using dispersed ozone flotation. While air aeration does not, ozone produces effective solid-liquid separation through flotation. Ozone dose applied for sufficient algal flotation is similar to those used in practical drinking waterworks.

Strategies to enhance cell growth and achieve high-level oil production of a Chlorella vulgaris isolate.

The autotrophic growth of an oil-rich indigenous microalgal isolate, identified as Chlorella vulgaris C--C, was promoted by using engineering strategies to obtain the microalgal oil for biodiesel synthesis. Illumination with a light/dark cycle of 14/10 (i.e.

Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil-contaminated site.

Rhamnolipid is one of the most effective and commonly used biosurfactant with wide industrial applications. Systematic strategies were applied to improve rhamnolipid (RL) production with a newly isolated indigenous strain Pseudomonas aeruginosa EM1 originating from an oil-contaminated site located in southern Taiwan. Seven carbon substrates and four nitrogen sources were examined for their effects on RL production.