[Are the properties of peripheral blood lymphocytes of patients with prostate cancer connected with the efficiency of radiation therapy?].

The genome damage (frequency of cells with micronuclei and chromosome aberrations), concentration of reactive oxygen forms (ROS), markers of lymphocytes activation, expression of proliferation (CD69, Ki67) and proapoptotic antigen (CD95), as well as the ability to adaptive response have been investigated in blood lymphocytes of healthy donors and patients with prostate gland cancer. The influence of hormone-therapy on lymphocytes properties and connection between the parameters studied with the effectiveness of treatment, which was estimated by the level of prostate specific antigen (PSA), have been investigated. It was discovered that the genome damage to the patients with prostate gland cancer lymphocytes does not differ from control.

[Individual variability of immunological markers, radiosensitivity and oxidative status in blood lymphocytes of Moscow residents].

Expression of activation (CD69) and proliferation (Ki67) markers, their connection with each other, with the oxidative status (reactive oxygen species--ROS) and with radiosensitivity (determined by micronucleus test) have been studied on stimulated blood lymphocytes from Moscow inhabitants. It was shown that the content of T-lymphocytes with the expressed CD69 and the content of T-lymphocytes with the expressed Ki67 markers correlate (r = 0.571; p = 0.

[Cytogenetic and immunological characteristics of stimulated human peripheral blood lymphocytes are connected with cell proliferation rates].

In the current study the authors investigated the connection between the proliferation rate of stimulated hu- man peripheral blood lymphocytes with some other characteristics of the population: expression of immunological markers, spontaneous genome damage, radiosensitivity. The population's proliferation index (PI) was taken as a measure of the rate. It was calculated using the composition of a cell population, which was cyto- kinesis-blocked with a cytochalasin B.

[Changes in cellular radiosensitivity after low dose irradiation].

When the adaptive response (AR) was studied on human blood lymphocytes, a new dependence was discovered. This dependence defines the direction of the radiosensitivity change after a low dose of irradiation. Using micronucleus (MN) test with cytochalasin B the dependence between the cell reaction after low level irradiation and radiosensititvity (the effect after irradiation at the dose of 1 Gy) was observed.

[The disturbances of the relationship between oxidative homeostasis and the immune status of lymphocytes in liquidators of the consequences of the Chernobyl accident].

We investigated into the relations between the immune status of individuals who took part in liquidating the consequences of the Chernobyl accident (liquidators) and the level of active oxygen forms in peripheral blood lymphocytes, as well as the level of the genome damage in lymphocytes (frequency of cells with micronuclei). The results show that the immune status changes as the level of the genome damage increases: the content of some markers increases and others decreases. It has also been shown that a) active oxygen forms participate in forming some indexes of the immunological lymphocyte status and b) the exposure of liquidators to irradiation many years ago almost completely changes the characteristics of the relation between the concentration of active oxygen forms and immunological status indexes.

[Molecular-biological properties of blood lymphocytes of Hodgkin's lymphoma patients. Plausible possibility of treatment effect prognosis].

Hodgkin's lymphoma (HL) patients were investigated before and during chemical and radiation therapy. The properties of peripheral blood lymphocytes of the HL patients before treatment have been compared with healthy donors and the patients during the treatment. The genetic damage--frequency of cells with micronuclei (MN), the level of DNA single- and double-strand breaks (SSB and DSB), DNA-protein cross-links (DPC) have been studied.

[Adaptive response of blood lymphocytes as a marker of hemopoiesis status in exposed persons].

An obvious correlation between the type of reaction manifested by peripheral blood lymphocytes to low dose irradiation in vitro (adaptive potential), the RBM cell composition (during the period of the major exposure), and the peripheral blood cell composition (at a late time period coincident with the studies of induced radioresistance) has been found in the Techa riverside residents in the later periods after the onset of a long-term low-dose rate radiation exposure (55-60 years later) within a range of individual red bone marrow doses from 0.01 to 1.79 Gy.

[Molecular and cellular consequences of the Chernobyl accident].

In this paper the results of the Chernobyl accident investigation 5-10 and 24 years after are summarized. The genomic instability, adaptive response formation, genome damage and oxidative status have been investigated. The studies were performed on cells in culture, mice, children and adults living in contaminated areas and liquidators.

[Distribution of individuals by spontaneous frequencies of lymphocytes with micronuclei. Particularity and consequences].

By micronucleus (MN) assay with cytokinetic cytochalasin B block, the mean frequency of blood lymphocytes with MN has been determined in 76 Moscow inhabitants, 35 people from Obninsk and 122 from Chelyabinsk region. In contrast to the distribution of individuals on spontaneous frequency of cells with aberrations, which was shown to be binomial (Kusnetzov et al., 1980), the distribution of individuals on the spontaneous frequency of cells with MN in all three massif can be acknowledged as log-normal (chi2 test).

[Molecular-cellular characteristic of blood lymphocytes in Hodgkin lymphoma].

The molecular-cellular parameters complex has been studied on the blood lymphocytes of malignant Hodgkin's lymphoma (HL) patients: the frequency of cells with micronuclei (MN) and chromosome aberrations; the level of DNA single and double strand breaks - OR and DR DNA (DNA comet assay), oxidative status--the content of reactive oxygen species (ROS) by using nonfluorescent dye that is oxygenated in the cells to fluorescent reagent and detection of fluorescence intensity after there. It was shown that the patients with LH had the increased level of DR and OR DNA, the increased frequency of cells with chromosome aberrations and the number of aberrations per cell was increased too. The concentration of ROS is increased too for the most individuals with intoxication.

[The connection between molecular-cellular parameters and immune status of liquidators after Chernobyl accident].

The genome damage (the frequencies of cells with micronuclei (MN), chromosome aberrations, the level of DNA double-strand breaks (DSB DNA), the concentration of reactive oxygen species (ROS) and 28 immunological parameters have been studied on the blood lymphocytes of Chernobyl accident liquidators. The purpose of this article was the investigation of cytogenetic, molecular changes of blood lymphocytes of irradiated individuals 24 years after accident, examination it there are correlation between genome damage and immunological parameters. It was shown that in lymphocytes of liquidators the frequencies of cells with MN and with all type of chromosome aberrations didn't differ from the lymphocytes of nonirradiated individuals, but the frequency of chromosome aberration type was increased, the level of DSB DNA was increased too.

[Polymorphic markers G(-455)A of gene FGB and C(-1654)T of gene PROC and genetic predisposition to unfavorable outcomes patients undergoing acute coronary syndrome].

We investigated the association of polymorphisms of genes FGB G(-455)A and PROCC(-1654)T with coronary artery disease (CAD) in the Russian population. A total of 1145 patients with CAD diagnose on the basis of clinical studies in cardiological hospitals of Moscow, St. Petersburg, Kazan, Chelyabinsk, Perm, Stavropol and Rostov-on-Don.

[Genomic damage, adaptive response induction in blood lymphocytes at the prostate cancer patients. Connection with the tumour radiotherapy efficiency].

On the blood lymphocytes of prostate cancer (PCa) patients before and during radiotherapy: DNA damage by DNA commet assay (DNA double strand breaks - DSB); the frequency of cells with micronuclei (MN) with cytokinetic cytochalasin block; the adaptive response induction by the additional irradiation of PHA stimulated lymphocytes in the doses of 0.05 and 1.0 Gy 24 h and 48 h after stimulation were studied.

[Adaptive response in the different mitotic cycles after irradiation].

The frequency of cells with chromosome aberrations and the number of aberrations per cell by metaphase analysis have been studied in the nonirradiated progeny of irradiated human blood lymphocytes. The DNA fragmentation (DNA double strand breaks) have simultaneously been investigated by the DNA comet assay. PHA stimulated lymphocytes have been irradiated in the adaptive dose 0.

[The radiosensitivity change of human blood lymphocytes in different mitotic cycles after a low dose irradiation].

The adaptive response (AR) in stimulated blood lymphocytes (8 donors) have been studied by two methods: analyses of unstable chromosome aberrations in metaphases and micronuclei assay (MN) with cytochalasine cytokinetic block. The adaptive irradiation in the dose of 5 cGy have been conducted 24 h and challenge irradiation after 48 h after stimulation. For the metaphase analysis of the first and subsequent mitosis cells were incubated with BrdU and were fixed 50, 72 and 96 h after the stimulation.

[The adaptive response of first generation offsprings irradiated parents].

In offspring's of first generation irradiated inhabitants of Techa river (fathers, mothers and both parents) the spontaneous level of damaged blood lymphocytes, sensitivity of lymphocytes to the additional acute irradiation in dose 1.0 Gy and radioinduced adaptive response after adaptive (5 cGy) and challenge (1.0 Gy) irradiation 5 h after was studied.

[Individual cytogenetic and molecular-biological characteristics of lymphocytes in blood of pilots and cosmonauts].

The purpose of this paper was the investigation of the pilots and of cosmonauts individual sensitivity to the fly conditions, to the additional irradiation (in the dose of 1 Gy), the adaptive response manifestation (in the doses 0.05 and 0.5 Gy).

[On the response of a cell population to low-dose irradiation].

The cell composition of a population of human blood lymphocytes was studied after irradiation at doses of 5 cGy, 1.0 Gy and 5 cGy + 1.0 Gy and the use of a cytokinesis block.

[Effect of low dose irradiation on the reaction of blood lymphocytes of individuals with the somatic diseases].

The reaction of blood lymphocytes on adaptive irradiation in vitro (0.05 Gy) and challenge irradiation (1.0 Gy) 5 h after has been studied among 320 children 3-16 years old by micronuclei test with cytochalasin B cytokinetic block.

[On the new mechanism of adaptive response].

The role of changes in cell composition of population of human blood lymphocytes in the forming of an adaptive response (AR) has been studied. By micronuclei assay and cytokinetic block with cytohalasin B the frequency of mono-, bi- and multinuclear cells with micronuclei (MN) and without MN were determined in the initial population. The same parameters have been studied after exposure of the population to the adaptive (0.

[Adaptive response of blood lymphocytes of the inhabitants of the South Ural chronically exposed to radiation].

The inhabitants of Techa river villages exposed to injured radiation action and inhabitants of uncontaminated regions were examined by micronuclei (MN) assay. The initial damage of blood lymphocytes, the role of radiation in the induction of sensitivity to the acute irradiation and the ability to form the adaptive response were evaluated. It was shown that the initial level of damaged lymphocytes in the inhabitants of the contaminated area did not differ significantly from the spontaneous level.

[The cytogenetic damage, radiosensitivity and adaptive response in children living in the different districts of Moscow].

The spontaneous level of blood lymphocytes with micronuclei (MN), the sensitivity to 1.0 Gy irradiation and adaptive response (AR) after adaptive irradiation with a dose of 0.05 Gy 5 hr later have been studied in children population living in different districts of Moscow.

[The reaction of cell population to low level of irradiation].

The results of long-term investigations of the effectivity of low level irradiation (below 0.5 Gy) carried out on the cells in culture and blood human lymphocytes (adults and children) have been brought. In the experiments conducted in the laboratory conditions and in the contaminated with radionuclides regions (after Chernobyl accident) the genomic instability have been discovered.

[The cell composition in the lymphocyte population and the radiation adaptive response].

Using lymphocytes of 5 healthy individuals the ability to adaptive response (AR), cell composition of population after PHA stimulation, changes in cell composition population after irradiation in the dose of 1.0 Gy and after irradiation in adaptive (0.05 Gy) and challenge (1.