[The toxic action of the "diabetogenic factor" metabolite in the body during the development of insulin-dependent diabetes].

Investigations of "diabetogenic factor" (DgF) (Diploma No. 386, USSR) in the blood of men and animals with insulin-dependent diabetes have been summarized. The nature and properties of the DgF purified preparation have been outlined and its pathophysiological importance characterized.

[Prophylactic effect of vitamin A, neutralizing the development of experimental insulin-dependent diabetes in animals].

Vitamin A deficiency contributed to higher incidence of abnormalities in experimental animals with insulin-dependent diabetes induced by alloxan. However, the similar doses of alloxan did not cause diabetes in the animals maintained on a diet containing adequate amounts of vitamin A used for prophylactic purposes for a long time. The natural diabetogenic factor specific to insulin-dependent diabetes was not found in the blood serum of these animals.

[Fibrinolytic and anticoagulant complexes of low-molecular heparin with tuftsin].

The complex of immunopeptide taftsin with low-molecular heparin has been obtained. The complex has fibrinolytic and anticoagulant activities in vitro and in vivo after the injection to albino rats.

[Low-molecular heparin complex formation with the blood coagulation proteins--thrombin and fibrinogen].

It has been found that low molecular heparin (LMH) forms complexes with fibrinogen and thrombin. The formation of the heparin-fibrinogen and heparin-thrombin complexes has been testified by cross-linked electrophoresis. The reaction of complex formation was carried out at variable weight ratios of the components, i.

[Fibrinolytic complexes of low-molecular heparin and acetylsalicylic acid].

Complexes of low-molecular heparin with acetylsalicilic acid was formed in vitro when the weight ratio of components was 1:1, 1:5 and 5:1, respectively. All the complexes possessed fibrinolytic and anticoagulating activities. The complex possessed the highest activity when the ratio of heparin to acetylsalicilic acid was 5:1.

[A comparative study of the action of preparations of high- and low-molecular weight heparin on hemostatic indices in vitro and in their intravenous administration to animals].

Low-molecular heparin preparations (a Soviet sample and that manufactured by the Celsus Company) in vitro, added to bovine plasma possess a lower antifactor-II activity, less time of recalcification and partial thromboplastin time as compared to high-molecular heparin of equal concentration (mg/ml) or dose (Units/ml). After intravenous injection of low-molecular heparin preparations to animals in a dose of 50 Units/200 g bw the time of hemorrhage was far less, while anticoagulant activity was lower than in animals given the same dose of high-molecular heparin. Both injection of low-molecular and high-molecular heparin results in a rise of the intensity of non-enzymatic fibrinolysis in the animals' blood plasma.

[Heparin from the meadowsweet (Filipendula ulmaria) and its properties].

In the present work, the nature of an anticoagulant from Philipendula ulmaria was studied. A method for purification of this anticoagulant was developed. Using diverse methods it was shown that the molecular weight, data on element (sulphur, nitrogen, and hydrogen) content, spectral characteristics in the infrared region of the spectrum, and electrophoretic properties of the product indicate its similarity to heparin of animal origin.

[The action of regulator peptides with different biological activities on the process of fibrin-monomer polymerization and on nonenzymatic fibrinolysis].

It is shown that preparations of regulatory peptides (RP): interleukin-1, thymoptin, and the endogenous nonpeptide opioid salsalinol produce a marked depolymerization effect on the fibrin-monomer and display nonenzymatic fibrinolytic activity in relation to unstabilized fibrin. Preparations of regulatory peptides thymalin and diphensin of rabbits intensify fibrin polymerization. A single intravenous infusion of diphensin preparation inhibits nonenzymatic fibrinolysis of blood plasma in vivo and in vitro.

[The blood anticoagulant system in rats perorally administered a heparin-acetylsalicylic acid complex].

Heparin/acetylsalicylate complexes (1:9 and 10:1) were obtained in vitro. Single or chronic (7-8 days) per os administration to white rats of 0.1% solution of the heparin/acetylsalicylate complex (0.

[Stable reduction of endogenous insulin production in the body in experimental insulin-dependent diabetes].

Implantation of beta-cells allogenic culture into animals with alloxan diabetes did not produce persistent positive effect. The implanted beta-cells lost their viability as a result of toxic effect of natural diabetogenic factor occurring in blood plasma during insulin-dependent diabetes. Long-term administration of heparin into these animals within first 90 days of the experiment enabled to avoid the negative phenomenon and to neutralize the diabetogenic factor activity.

[The effect of tuftsin on fibrin polymerization in vitro and on the hemostatic system when administered intravenously to healthy animals].

Taftsine tetrapeptide has antiprocoagulatory properties in vitro in the presence of plasma. Taftsine exerts depolymerization influence on fibrin monomer in concentrations of 10(-1) to 10(-9) mg/ml. At intravenous injection in doses of 1 mg and 300 ug/kg, taftsine causes the increase in plasma clotting time and significant increase in enzymatic and nonenzymatic fibrinolysis.

[The pancreas as a source of natural diabetogenic factor in alloxan diabetes].

The results of experimental studies bear evidence that the pancreas of healthy animals produces a humoral factor which differs from insulin and prevents the development of alloxan diabetes. The pancreas of diabetic animals loses the above-mentioned activity and produces into the blood plasma a natural diabetogenic factor which promotes the development of alloxan diabetes.

[The effect of regulatory peptides with various biological activity on the fibrin polymerization process and non-enzymatic fibrinolysis. Hypophyseal and hypophthalamic hormones, liberin, ACTH, and its analogs].

Preparations of regulating peptides from hypophysis oxytocin (10(-1)-10(-3) mg/ml) and vasopressin (10(-2)-10(-5) mg/ml) demonstrated weak depolymerizing effect on unstabilized fibrin. Regulating peptides from hypothalamus thyroliberin and PR-546 (10(-1)-10(-3) mg/ml) increased polymerization of fibrin-monomer.

[The antiheparin thrombocyte factor 4 and its interaction with heparin].

A rat platelet factor has a high antiheparin activity. It also decreases nonenzymatic fibrinolytic activity of normal rat plasma and antithrombin III-heparin complex. The platelet factor 4 formed inactive complexes with heparin in molar ratios of 1:1 and 2:1.

[Process of fibrin depolymerization and non-enzymatic fibrinolysis in rabbits receiving atherogenic rations with addition of antioxidants].

The rabbits were kept on atherogenic ration for 2 months. This diet contained 0.3 mg/kg of cholesterol.

[Heparin-histamine complex, its physico-chemical and biological properties].

Complexes between heparin and histamine at various ratio exhibited various physiological activity. The complex containing heparin-histamine at the ratio of 6:1, 10:1 or 15:1 showed anticoagulation, antipolymerization and nonenzymatic fibrinolytic effects. The complex dissociated in circulation within 90 min after its administration.

[The content of a heparin-like anticoagulant in the flowers of the meadowsweet (Filipendula ulmaria)].

The flowers of Filipendula ulmaria were found to contain heparin bound to the plant proteins in the form of a complex. This complex enhances the anticoagulant and fibrinolytic properties of the nonenzymatic nature at its administration to animals both intramuscularly and intravenously. The neutralizing effect of protaminesulphate on the anticoagulant activity of the plant heparin was shown.

[Platelet aggregation induced by fibrinmonomer and the effect of the heparin-adrenaline complex or plasmin on this process].

Addition of purified fibrin-monomer in a concentration of 0.5 mg/ml induces aggregation in a suspension of washed rat platelets in the absence of aggregants. Maximum aggregation takes place 1-3 min after fibrin-monomer addition, and then the disaggregation phase follows.

[The action of timoptin peptides on the hemostatic system].

Repeated introduction of thymus polypeptide drug thymoptine (8 times every 24 hours, 1 microgram/kg) led to the increase in total and non-enzymatic fibrinolytic activity of blood plasma and the decrease in fibrinogen concentration. Thymoptine could lyse non-stabilized fibrin and cause depolymerization of aggregates of fibrin monomer in virto. Highest depolymerization activity was observed at thymoptine concentration of 10 micrograms/ml.

[Recovery of the anticoagulation system function in patients with insulin-dependent diabetes by neutralization of the activity of diabetogenic factor].

Functions of the anticoagulation system were restored in patients with insulin-dependent diabetes after neutralization of the diabetogenic factor in blood of patients by repeated administration of heparin at low doses. Development of thrombosis was also prevented in these patients.

[Effect of defensin on the process of healing of aseptic skin wound and on the permeability of blood vessels].

It was shown, for the first time, that polypeptide of rabbit's neutrophils, defensin (D) has the ability to accelerate the reparation process (RP). D was infused intramuscular to rats (125 micrograms/kg) 2 days before the operation, then the skin on the back was dissected through all layers (the length of the wound was constant-10 mm). The rate of the RP was estimated by the changes of the wound length on 2, 5, 7, 13-15 days after the operation.

[C-peptide, diabetogenic factor and the blood anticoagulating system in patients with the insulin-dependent type of diabetes mellitus before and after heparin therapy].

The authors analyzed the results of investigation of insulin residual secretion determined by the concentration of C-peptide in response to the stimulation of 1 mg of glucagon. The blood level of the diabetogenic factor (DGF) and activity of the anticoagulative system (ACS) were studied in parallel in patients with insulin-dependent type of diabetes mellitus before and after heparin therapy. The blood DGF disappeared, ACS function was restored, and the patient's body resistance to blood hypercoagulation developed against a background of heparin therapy.

[A method of determining the anti-, de- and fibrin-polymerization activity of the blood plasma].

The suggested method is based on measuring the changed level of polymerized fibrin monomer and nonstabilized fibrin under the effects of agents enhancing or inhibiting fibrin monomer polymerization. This method permits measurement of plasma specific activity influencing fibrin monomer and unstabilized fibrin polymerization in human and animal blood plasma in health and various diseases involving disorders of hemostasis system, as well as in various drug exposures.