LMVSegRNN and Poseidon3D: Addressing Challenging Teeth Segmentation Cases in 3D Dental Surface Orthodontic Scans.

The segmentation of teeth in 3D dental scans is difficult due to variations in teeth shapes, misalignments, occlusions, or the present dental appliances. Existing methods consistently adhere to geometric representations, omitting the perceptual aspects of the inputs. In addition, current works often lack evaluation on anatomically complex cases due to the unavailability of such datasets.

Different factors limit early- and late-season windows of opportunity for monarch development.

Seasonal windows of opportunity are intervals within a year that provide improved prospects for growth, survival, or reproduction. However, few studies have sufficient temporal resolution to examine how multiple factors combine to constrain the seasonal timing and extent of developmental opportunities. Here, we document seasonal changes in milkweed ()-monarch () interactions with high resolution throughout the last three breeding seasons prior to a precipitous single-year decline in the western monarch population.

Risk Assessment of Hospitalized Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)-Infected Patients Using Laboratory Data and Immune Cell Morphologic Assessment.

Context.—: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly infectious agent, with the propensity to cause severe illness. While vaccine uptake has been increasing in recent months, many regions remain at risk of significant coronavirus disease 19 (COVID-19)-related health care burden.

Engineered Cells as a Test Platform for Radiohaptens in Pretargeted Imaging and Radioimmunotherapy Applications.

Pretargeted imaging and radioimmunotherapy approaches are designed to have superior targeting properties over directly targeted antibodies but impose more complex pharmacology, which hinders efforts to optimize the ligands prior to human applications. Human embryonic kidney 293T cells expressing the humanized single-chain variable fragment (scFv) C825 (huC825) with high-affinity for DOTA-haptens (293T-huC825) in a transmembrane-anchored format eliminated the requirement to use other pretargeting reagents and provided a simplified, accelerated assay of radiohapten capture while offering normalized cell surface expression of the molecular target of interest. Using binding assays, biodistribution, and imaging, we demonstrated that radiohaptens based on benzyl-DOTA and a second generation "Proteus" DOTA-platform effectively and specifically engaged membrane-bound huC825, achieving favorable tumor-to-normal tissue uptake ratios in mice.

Aedes aegypti miRNA-33 modulates permethrin induced toxicity by regulating VGSC transcripts.

Aedes aegypti is a major vector of Zika, dengue, and other arboviruses. Permethrin adulticidal spraying, which targets the voltage-gated sodium channel (VGSC), is commonly done to reduce local mosquito populations and protect humans from exposure to arbovirus pathogens transmitted by this dangerous pest. Permethrin resistance, however, is a growing problem and understanding its underlying molecular basis may identify avenues to combat it.

TERT-Regulation and Roles in Cancer Formation.

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. Telomerase complex plays a key role in cancer formation by telomere dependent or independent mechanisms. Telomere maintenance mechanisms include complex changes such as gene amplifications, structural variants, promoter germline and somatic mutations, epigenetic changes, and alternative lengthening of telomere.

Novel 3D heart left ventricle muscle segmentation method for PET-gated protocol and its verification.

Objective: The aim of this study was to propose and verify a universal method of left ventricular myocardium segmentation, able to operate on heart gated PET data with different sizes, shapes and uptake distributions. The proposed method can be classified as active model method and is based on the BEAS (B-spline Explicit Active Surface) algorithm published by Barbosa et al. The method was implemented within the Pmod PCARD software package.

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti.

Association mapping of factors that condition pyrethroid resistance in Aedes aegypti has consistently identified genes in multiple functional groups. Toward better understanding of the mechanisms involved, we examined high throughput sequencing data (HTS) from two Aedes aegypti aegypti collections from Merida, Yucatan, Mexico treated with either permethrin or deltamethrin. Exome capture enrichment for coding regions and the AaegL5 annotation were used to identify genes statistically associated with resistance.

Sub-endocardial and sub-epicardial measurement of myocardial blood flow using NH PET in man.

Background: This study examined whether measuring myocardial blood flow (MBF) in the sub-endocardial (SEN) and sub-epicardial (SEP) layers of the left ventricular myocardium using NH positron emission tomography (PET) and an automated procedure gives reasonable results in patients with known or suspected coronary artery disease (CAD).

Methods: Resting and stress NH dynamic PET were performed in 70 patients. Using ≥ 70% diameter stenosis in invasive coronary angiography (ICA) to identify significant CAD, we examined the diagnostic value of SEN- and SEP-MBF, and coronary flow reserve (CFR) vs.

Implementation and Sustainability of European Training Program on Organ Donation in Poland: Results and the Impact on Donation Indicators.

Background: Hospital training called ETPOD-Essentials in Organ Donation-was introduced in Poland in 31 hospitals with under-utilized potential of donation. The aim of this study was to assess the effect in hospitals included and not included in program, before and after trainings.

Methods: The number of potential and effective donors, organs used, and number (%) of family refusals were compared at 10 and in 20 months after the training and in equal periods before.

The Model of Postgraduate Studies for Transplant Coordinators in Poland: 500 Graduates in the Years 2007-2015.

Background: Since 2007, the Medical University of Warsaw has been organizing the Transplant Coordinators Postgraduate Studies, which are funded by the National Programme for the Development of Transplantation Medicine. The aim of the studies is to recruit medical professionals for every hospital with the potential of deceased donations. The goal of the present study was to formally evaluate the previous 16 editions of Transplant Coordinators Postgraduate Studies by assessing graduate profiles and analyzing the efficiency and sustainability of their education.

Partnership for transplantation: a new initiative to increase deceased organ donation in Poland.

Despite the long-standing history of transplantation, the shortage of organs has remained its most restrictive factor. In 2010, the number of actual deceased organ donors in Poland was 13.5/million population (pmp).

[The skin incisions (blow holes) for treatment of massive subcutaneous emphysema].

Background: Subcutaneous emphysema (SE) is rarely life-threatening, although it may create significant discomfort to patients. It may impede eye opening, movement of the limbs and sometimes causes stridor and respiratory distress. We describe two cases of SE, in which small incisions in the skin helped to relieve symptoms.

Educational system for transplant coordinators in Poland: postgraduate studies at Warsaw Medical University; 2 years of experience.

Donor hospital transplant coordinators play crucial roles in the donation process. There are only a few coordinators in Poland, while there are about 400 hospitals with intensive care units (ICU). Coordinators must be professionals in medical sciences, clinical psychology, law, organization, management, and statistics.

[Cardiac arrest in chronic metabolic alkalosis due to sodium bicarbonate abuse].

Background: Moderate metabolic alkalosis has not been considered as a life-threatening situation by many authors, but when it persists and pH increases above 7.65, the situation may become critical.

Case Report: We present a case of a 61-yr-old alcoholic male patient, who had been consuming approximately 200 g of sodium bicarbonate daily for twenty years, due to persisitent heartburn and abdominal pains.

Nanotechnology on duty in medical applications.

At the beginning of 21(st) century, fifty years after discovery of deoxyribonucleic acid (DNA) double helix structure, scientific world is faced with a great progress in many disciplines of biological research, especially in the field of molecular biology and operating on nucleid acid molecules. Many molecular biology techniques have been implemented successfully in biology, biotechnology, medical science, diagnostics, and many more. The introduction of polymerase chain reaction (PCR) resulted in improving old and designing new laboratory devices for PCR amplification and analysis of amplified DNA fragments.

A modified mordant technique for staining plant chromosomes.

The ability to produce ferric acetate in house has provided a new mordant source for use in Newcomer's fluid (6:3:1:1:1 isopropanol, propionic acid, acetone, petroleum ether, 1,4-dioxane) for species with small chromosomes. This method improves on one first published in 1970.

Characterization of freezing tolerance and vernalization in Vern-, a spring-type Brassica napus line derived from a winter cross.

Exposure of winter brassicas to periods of low temperature decreases the transition required from vegetative to reproductive growth. This low-temperature exposure also results in the acquisition of freezing tolerance. To investigate the relationship between vernalization and freezing tolerance, homozygous microspore-derived (DH) lines were developed from reciprocal crosses between two winter cultivars of Brassica napus L.

Optimizing the assessment of age-related changes in trabecular bone.

The goal of this study was to develop an optimal procedure to determine age-related changes in trabecular bone. The investigations were based on two-dimensional images of the human vertebral trabecular bone specimens. The following indices of trabecular structure were considered: bone volume/total volume, star volume of the marrow cavity, Euler number and the probability of disconnection (straightforwardly connected with the number of separated parts of the network).

[Double intertrochanteric distraction osteotomy.].

The authors present four case-histories pertaining to double intertrochanteric distraction osteotomy of the proximal portion of the femur. In two female patients the result was excellent, in one female patient a 130 degrees splint broke and in another the splint was bent and the splints had to be replaced. All patients are satisfied with the operation because the extremity was prolonged on average by 2 cm.

Potentiometric determination of chlorides with an "Air-Gap" cyanide sensor.

A new electroanalytical method for determining chloride ions with an Air-Gap cyanide sensor system is described. The method is based on the reaction of chloride with mercury(II) cyanide in dilute sulphuric acid. This reaction leads to hydrogen cyanide which can be determined with an Air-Gap cyanide sensor.