Enhancing Dental Applications: A Novel Approach for Incorporating Bioactive Substances into Textile Threads.

In the realm of surgical and dental applications, hyaluronic acid (HA) braided threads show significant therapeutic potential due to their incorporation of pharmaceutical active ingredients. This study primarily focuses on resolving the crucial challenge of devising a deposition method that can ensure both precision and uniformity in the content of the active ingredient Octenidine dihydrochloride (OCT) within each segment of the threads. Our objective in this study was to develop a continuous deposition method for OCT onto a braided thread composed of 24 hyaluronic acid-based fibers, aiming for a specific OCT content of 0.

Nonwoven Textiles from Hyaluronan for Wound Healing Applications.

Nonwoven textiles are used extensively in the field of medicine, including wound healing, but these textiles are mostly from conventional nondegradable materials, e.g., cotton or synthetic polymers such as polypropylene.

Screening of Phenanthroquinolizidine Alkaloid Derivatives for Inducing Cell Death of L1210 Leukemia Cells with Negative and Positive P-glycoprotein Expression.

We describe the screening of a set of cryptopleurine derivatives, namely thienoquinolizidine derivatives and (epi-)benzo analogs with bioactive phenanthroquinolizidine alkaloids that induce cytotoxic effects in the mouse lymphocytic leukemia cell line L1210. We used three variants of L1210 cells: i) parental cells (S) negative for P-glycoprotein (P-gp) expression; ii) P-glycoprotein positive cells (R), obtained by selection with vincristine; iii) P-glycoprotein positive cells (T), obtained by stable transfection with a human gene encoding P-glycoprotein. We identified the most effective derivative with a median lethal concentration of ≈13 μM in all three L1210 cell variants.

Detection of the Mitochondrial Membrane Potential by the Cationic Dye JC-1 in L1210 Cells with Massive Overexpression of the Plasma Membrane ABCB1 Drug Transporter.

JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 in these organelles leads to the formation J-aggregates (with a specific red fluorescence emission maximum at 590 nm), which is in addition to the typical green fluorescence of J-monomers (emission maximum of ∼529 nm). The lack of mitochondrial ΔΨ leads to the depression of JC-1 mitochondrial accumulation and a decrease in J-aggregate formation.

Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells.

Aims: Haloperidol is an antipsychotic agent and acts as dopamine D2 receptor (D2R) antagonist, as a prototypical ligand of sigma1 receptors (Sig1R) and it increases expression of type 1 IP receptors (IPR1). However, precise mechanism of haloperidol action on cardiomyocytes through dopaminergic signaling was not described yet. This study investigated a role of dopamine receptors in haloperidol-induced increase in IPR1 and Sig1R, and compared physiological effect of melperone and haloperidol on basic heart parameters in rats.

Haloperidol Affects Plasticity of Differentiated NG-108 Cells Through σ1R/IPR1 Complex.

Haloperidol is an antipsychotic agent that primarily acts as an antagonist of D2 dopamine receptors. Besides other receptor systems, it targets sigma 1 receptors (σ1Rs) and inositol 1,4,5-trisphosphate receptors (IPRs). Aim of this work was to investigate possible changes in IPRs and σ1Rs resulting from haloperidol treatment and to propose physiological consequences in differentiated NG-108 cells, i.

Slow sulfide donor GYY4137 differentiates NG108-15 neuronal cells through different intracellular transporters than dbcAMP.

Cellular differentiation is the process, by which a cell changes from one cell type to another, preferentially to the more specialized one. Calcium fluxes play an important role in this action. Differentiated NG108-15 or PC12 cells serve as models for studying neuronal pathways.

Possible participation of EDRF-NO in the hormonal regulation of bone blood flow in rats.

An increase in bone blood flow (BBF) was observed in rats after castration whereas a decrease in BBF occurred after oestradiol or testosterone. The possible participation of prostaglandins in these changes was demonstrated. The present results show that the endothelium-derived relaxing factor, i.

[DHEA: another hormonal modulator of bone blood flow in rats].

Dehydroepiandrosterone (DHEA) is a steroid with important effects on the bone tissue. We tried to check up if it influenced also the bone blood flow (similarly as estradiol and testosterone). We administered DHEA (Sigma) dissolved in dimethylsulphoxide s.

[The role of EDRF-NO in the regulation of bone blood flow in rats: inhibition with L-NAME].

EDRF-NO probably participates-besides the prostaglandins [3, 4]-in local circulatory changes in the bones of female rats with modified level of sex hormones; we could demonstrate it indirectly using methylene blue as a blocking agent [5]. In this paper, we present corresponding results of two experiments with NG-nitro-arginine methyl ester (L-NAME) as a substance blocking the production of endothelium derived relaxing factor, i.e.

[Further evidence of the role of prostaglandin on the effects of hormones on blood circulation in bones].

In a short communication we demonstrated the inhibitory affect of acetylosalicylic acid (ASA) on the increased bone blood flow and 45Ca and 3H-proline incorporation in oophorectomized (OOX) female rats. This finding suggested probable participation of prostaglandin. The aim of the present work was to confirm and extend the initial observation and to find out, whether the administration of ASA did not affect also the decrease in the bone blood flow after the administration of estradiol benzoate (EB).

Inhibitory effect of hydrocortisone on the blood flow and 45Ca and 3H-proline incorporation into bones of female rats.

We studied the effects of hydrocortisone as a possible regulatory factor of bone blood flow and metabolism. Local bone blood flow in the tibia, distal femur, lumbar vertebra and some soft tissues (using 85Sr-microspheres), as well as 45Ca and 3H-proline incorporation into the tibia, bone density and ash weight per ml of the tibia were measured in sham-operated and oophorectomized female rats in which the influence of hydrocortisone administration (0.004% diet for 5 weeks) was followed.

[A decrease in blood flow and incorporation of 45Ca and 3H-proline in the bones in pregnant female rats].

In the experiments on rats, the bone blood flow may be influenced by the deficiency as well by the administration of sex hormones. The aim of this work was to investigate the bone circulation (determined by means of 85Sr-microspheres, experiment A and B) and the incorporation of 45Ca and 3H-proline into the tibia (experiment C) in the last stage of pregnancy (from 18 to 21 days), i.e.

Blood flow and mineral content of the tibia of female and male rats: changes following castration and/or administration of estradiol or testosterone.

We studied changes in the local circulation and mineralization of the rat tibia under different experimental conditions. Four experiments were performed on a total of 155 female and male rats: after oophorectomy (OOX) or orchidectomy (ORX), after the administration of estradiol benzoate (EB, Agofollin Depot, 1 mg/rat once a week or 5 mg/kg body weight once every 5 days for 4 weeks), or after the administration of testosterone (T, Agovirin Depot, 25 mg/kg body weight once every 5 days for 4 weeks). We estimated 85Sr-microsphere uptake and blood flow in the tibia, density of the tibia, and ash weight per bone volume unit.

Relationships between local circulatory changes in the tibia and kidney of male and female rats after castration and administration of estradiol or testosterone.

The effects of castration and of the four weeks' administration of estradiol or testosterone on the blood circulation in the tibia and the kidney (expressed as the 85Sr-microsphere uptake values, which are not influenced by simultaneous changes in cardiac output) were studied in four experiments on a total of 147 rats (68 females, 79 males), relative weights being noted at the same time. 85Sr-microsphere uptake in the tibia rose markedly after castration in both males and females, fell after estradiol benzoate in intact females and intact and orchidectomized males and also fell after testosterone in intact and oophorectomized females and orchidectomized males. 85Sr-microsphere uptake in the kidney rose after castration only in males in experiments B; it fell after estradiol in orchidectomized males and fell after testosterone in intact females and males and in orchidectomized males.

[The effect of estradiol, norethisterone and testosterone on regional circulation and relative weight of the testes, ovaries and uterus in rats].

In nine experiments of 188 rats (85 males and 103 females), relative weight and local blood flow (with 85Sr-microspheres) was estimated in testes, ovaries and uterus after the administration of estradiol benzoate (Agofollin Depot 1 mg per rat or 5 mg per kg s.c. in time intervals of 3 to 7 days), norethisterone (Norethisterone tablets 0.

Possible participation of prostaglandins in the increase in the bone blood flow in oophorectomized female rats.

The aim of this study was to verify the possible role of prostaglandin (PG) in the increase in the bone blood flow of female rats after oophorectomy (OOX). In two experiments we determined blood flow in the tibia and distal femur (85Sr-microspheres) and 24-h incorporation of 45Ca and 3H-proline. Acetylosalicyclic acid (ASA, 0.

[Local blood circulation, uptake of 45Ca, 85Sr and 3H-proline and mineral levels in the tibia of female rats: comparison and changes after administration of estradiol].

After six weeks' administration of estradiol benzoate (Agofollin Depot, 1 mg s.c. once a week) we determined in the first part of 70 days old female rats the uptake of 85Sr-microspheres and local blood flow in the tibia, in the second part 24 hours' incorporation of 45Ca and 3H-proline and in the third part 2 hours' uptake of 85Sr, bone density and ash weight related to 1 ml of bone volume.

[Local blood circulation and bone mineral content in the bones of rats: the effect of castration, estradiol and testosterone in male and female rats].

Four experiments on 76 female and 79 male rats were performed in which we followed up the effect of four weeks' administration of estradiol benzoate (Agofollin Depot 1 mg per rat once a week or 5 mg per kg once every 5 days s. c.) and testosterone isobutyrate (Agovirin Depot 25 mg per kg once every 5 days s.

[Hydrocortisone decreases blood flow in bones in sham-operated and oophorectomized female rats].

In an experiment on 38 75-day-old female rats--sham-operated or oophorectomized (OOX)--the authors assessed the effect of hydrocortisone administration (0.01% in the diet for four weeks) on the blood flow in the tibia and distal end of the femur (uptake of 85Sr microparticles). In the tibia they assessed also the density (according to the Archimed principle) and the weight of ashes per volume unit of bone.

Further findings on the inhibitory effect of estradiol benzoate on the circulation and on 45Ca and 3H-proline incorporation in rat bones.

Various aspects of the effect of estradiol benzoate (EB, Agofollin Depot, Czechoslovakia, usually in a s.c. dose of 5 mg/kg body weight once a week), on the local circulation (the uptake of 85Sr-microspheres), the incorporation of 45Ca and 3H-proline, bone density and ash weight related to bone volume were studied in six experiments in the tibia and distal femur of 224 rats.

Different effects of oestradiol benzoate and norethisterone on the blood flow and mineral content in rat bones.

In three experiments (2 on females, 1 on males), we determined the blood flow in the tibia and the distal part of the femur, together with cardiac output (by means of 85Sr-microspheres), tibial bone density and tibial ash weight related to bone volume. We found that 1) the bone blood flow always fell significantly after oestradiol benzoate, 2) no change occurred after norethisterone in doses corresponding to those of oestradiol benzoate, but the blood flow showed a tendency to fall after doses one order higher (it decreased significantly in one case only), 3) the density of the tibia and tibial ash weight related to bone volume rose nonsignificantly after oestradiol benzoate, but fell (mostly statistically significantly) after norethisterone. The lowering of the bone mineral indexes in rat bones after norethisterone is a surprising and potentially significant finding requiring further verification.

[Increased blood flow and incorporation of 3H-proline and 45Ca into the bones of female rats after oophorectomy].

In the submitted experiment the authors investigated changes of the local circulation (by assessing retention of 85Sr microparticles) and incorporation of 3H-proline and 45Ca in bone of female rats following oophorectomy (OOX). Four weeks after OOX retention of 85Sr microparticles in % from 1 g tissue rises significantly in the tibia, distal epiphysis of the femur and in vertebrae (insignificantly in the metaphysis and calva); the blood flow in (1 min-1).kg-1 is significantly increased in the tibia and diaphysis of the femur (the differences are less significant as the scatter of values is greater).

[Reduced blood flow in bones in female rats after administration of estrogen].

The authors submit the results of investigations of the action of oestrogens on the blood flow in bones and other tissues of female rats (by means of 85Sr microparticles) on the incorporation of 3H-proline and 45Ca into bone marrow and on bone density and weight of the ashes. Daily s.c.

Estradiol benzoate decreases the blood flow through the tibia of female rats.

Effect of estradiol benzoate (EB, Agofollin Depot Spofa, CSSR, 1 mg s.c. once a week for 4 weeks) on local blood flow through the tibia of intact and oophorectomized (OOX) female rats was studied using the 85Sr-microsphere method.