Antiproliferative and Proapoptotic Activities of Marine Sponge Extract on Breast Carcinoma Cell Line (MCF-7).

Background: Marine sponge is a rich natural resource of many pharmacologically important compounds.

Objective: Marine sponge , collected from North Bay, South Andaman Sea, India, was screened for potential antiproliferative and proapoptotic properties on a breast adenocarcinoma cell line (MCF-7).

Materials And Methods: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to test the antiproliferative and cytotoxicity effects of the sponge extract.

Exploring GpG bases next to anticodon in tRNA subsets.

Transfer RNA (tRNA) structure, modifications and functions are evolutionary and established in bacteria, archaea and eukaryotes. Typically the tRNA modifications are indispensable for its stability and are required for decoding the mRNA into amino acids for protein synthesis. A conserved methylation has been located on the anticodon loop specifically at the 37(th) position and it is next to the anticodon bases.