Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems.

DNA amplification and reverse transcription enzymes have proven to be invaluable in fast and reliable diagnostics and research applications because of their processivity, specificity, and robustness. Our study focused on the production of mutant Taq DNA polymerase and mutant M-MLV reverse transcriptase in the expression hosts and under various expression conditions. We also examined nonspecific extracellular production in .

Tracking a spin-polarized superconducting bound state across a quantum phase transition.

The magnetic exchange coupling between magnetic impurities and a superconductor induce so-called Yu-Shiba-Rusinov (YSR) states which undergo a quantum phase transition (QPT) upon increasing the exchange interaction beyond a critical value. While the evolution through the QPT is readily observable, in particular if the YSR state features an electron-hole asymmetry, the concomitant change in the ground state is more difficult to identify. We use ultralow temperature scanning tunneling microscopy to demonstrate how the change in the YSR ground state across the QPT can be directly observed for a spin-1/2 impurity in a magnetic field.

Microwave excitation of atomic scale superconducting bound states.

Magnetic impurities on superconductors lead to bound states within the superconducting gap, so called Yu-Shiba-Rusinov (YSR) states. They are parity protected, which enhances their lifetime, but makes it more difficult to excite them. Here, we realize the excitation of YSR states by microwaves facilitated by the tunnel coupling to another superconducting electrode in a scanning tunneling microscope (STM).

Phase Ib trial of inhaled iloprost for the prevention of lung cancer with predictive and response biomarker assessment.

Introduction: Iloprost, a prostacyclin analog, has lung cancerpreventive activity in preclinical models and improved dysplasia in former smokers in a phase IIb trial. Oral iloprost is currently unavailable. We performed a phase Ib trial of inhaled iloprost in former smokers to assess tolerance and compliance.

Dynamical Coulomb Blockade as a Local Probe for Quantum Transport.

Quantum fluctuations are imprinted with valuable information about transport processes. Experimental access to this information is possible, but challenging. We introduce the dynamical Coulomb blockade (DCB) as a local probe for fluctuations in a scanning tunneling microscope (STM) and show that it provides information about the conduction channels.

Antibunched Photons Emitted by a dc-Biased Josephson Junction.

We show experimentally that a dc biased Josephson junction in series with a high-enough-impedance microwave resonator emits antibunched photons. Our resonator is made of a simple microfabricated spiral coil that resonates at 4.4 GHz and reaches a 1.

Emission of Nonclassical Radiation by Inelastic Cooper Pair Tunneling.

We show that a properly dc-biased Josephson junction in series with two microwave resonators of different frequencies emits photon pairs in the resonators. By measuring auto- and intercorrelations of the power leaking out of the resonators, we demonstrate two-mode amplitude squeezing below the classical limit. This nonclassical microwave light emission is found to be in quantitative agreement with our theoretical predictions, up to an emission rate of 2 billion photon pairs per second.

Thermal Conductance of a Single-Electron Transistor.

We report on combined measurements of heat and charge transport through a single-electron transistor. The device acts as a heat switch actuated by the voltage applied on the gate. The Wiedemann-Franz law for the ratio of heat and charge conductances is found to be systematically violated away from the charge degeneracy points.

From Coulomb-blockade to nonlinear quantum dynamics in a superconducting circuit with a resonator.

Motivated by recent experiments on superconducting circuits consisting of a dc-voltage-biased Josephson junction in series with a resonator, quantum properties of these devices far from equilibrium are studied. This includes a crossover from a domain of incoherent to a domain of coherent Cooper pair tunneling, where the circuit realizes a driven nonlinear oscillator. Equivalently, weak photon-charge coupling turns into strong correlations captured by a single degree of freedom.

Collective dynamics in optomechanical arrays.

Optomechanical systems couple light stored inside an optical cavity to the motion of a mechanical mode. Recent experiments have demonstrated setups, such as photonic crystal structures, that in principle allow one to confine several optical and vibrational modes on a single chip. Here we start to investigate the collective nonlinear dynamics in arrays of coupled optomechanical cells.

Violation of the Wiedemann-Franz law in a single-electron transistor.

We study the influence of Coulomb interaction on the thermoelectric transport coefficients for a metallic single-electron transistor. By performing a perturbation expansion up to second order in the tunnel-barrier conductance, we include sequential and cotunneling processes as well as quantum fluctuations that renormalize the charging energy and the tunnel conductance. We find that Coulomb interaction leads to a strong violation of the Wiedemann-Franz law: the Lorenz ratio becomes gate-voltage dependent for sequential tunneling, and is increased by a factor 9/5 in the cotunneling regime.