[Fine physical mapping of yeast chromosome V].

Electrophoretic karyotype of yeast strain A364a was obtained by pulsed field gel electrophoresis and the position of chromosome V on such karyotype was determined by means of dot hybridization with chromosome V-specific probe URA3. By cloning partially digested BamHI fragments of this chromosome DNA into integrative vector Yip5, a gene library specific to this chromosome was constructed. The number of the recombinants was much more than theoretically required.

Channel selection of atmospheric remote sensing.

We introduce a channel selection method for atmospheric remote-sensing problems described by a Fredholm integral equation of the first kind. Whether one set of channels (CH) is more suitable than another (CH') can be judged by whether (1) the degree of predominance (DP) value of CH is larger than that of CH', i.e.

[Study on the transferring of YACs to new host by means of KAR cross].

The donor yeast strain YAC23 containing a 340 kb human genomic DNA fragment in YAC (Yeast Artificial Chromosome) was mated with recipient strain YLB504 by means of improved kar cross and the condidate YACductants were assayed by PCR and the amplification band of 404 bp indicated that they were the same as those of recipient strains (i.e. MAT alpha).

[The "shen" reaction to trauma--an experimental study].

An experimental study using adult Kunming male mice was designed to observe the Shen reaction to trauma (bone fracture and burn). It was shown that the mice testes of the experimental groups were degenerated and the spermatogenesis disturbed. Under the electron microscope, the granules reduction, pyknosis, vesicular degeneration in the plasma of gonadotrophs and hemorrhage were found.

[The relation of lesions of the testis and acute injury and chronic disease of the kidney].

In this paper, the testicular routine sections of ninety autopsy cases of males between 20 and 29 years old were studied. The autopsies were performed in the department of pathology at the Bowman Gray School of Medicine of Wake Forest University in the United States. Primary pathological diagnoses were divided into acute injury (63 cases) and chronic disease (27 cases) groups.

Alpha-factor-directed synthesis and secretion of beta-subunit of human choriogonadotropin (beta-hCG) in yeast S. cerevisiae.

A couple of shuttle vectors (pYNA and pYNB), containing a sequence coding for the leader region of the alpha-factor precursor (alpha-factor leader sequence) and the origin of 2 micron circle, were constructed. The cDNA for the beta-hCG (145aa) was fused in phase to the 3'-end of the alpha-factor leader sequence and transformed into the yeast Saccharomyces cerevisiae. The yeast cells carrying this hybrid gene synthesized and secreted a protein with the immunoactivity of beta-hCG in an amount of 45 micrograms per liter of culture.

Assay of biological activity of synthetic yeast alanine transfer RNA (tRNAAlay).

The biological activity of the synthetic tRNAAlay was studied with an extremely sensitive method. tRNAAlay accepted alanine in the presence of rat liver aminoacyl-tRNAAlay-synthetase (this was called the accepting activity). The aminoacylated tRNAAlay was conveniently precipitated by ethanol with good recovery.

Small-cell carcinoma of the lung--survival according to histologic subtype: a Veterans Administration Lung Group Study.

Six-hundred-twenty cases of small-cell carcinoma of the lung entered into the Veterans Administration Lung Group protocols 9-15 were retrospectively subdivided into histologic subtype, as proposed by the WHO (1977). Medium survival was greater for subtype No. 21 (lymphocyte-like) than for subtype No.

Expression of gene(s) in restricted fragment of lambda DNA in E. coli.

Restriction endonucleases EcoR1 and BamH1 are used to produce fragments pBR322C (375 bp) and pBR322B (3987 bp) from pBR322, and to produce lambda F2A (65.6--71.3% of lambda DNA, 2679 bp) and lambda F2B (71.

Solvent extraction method used in separating the protected product of synthetic oligodeoxyribonucleotides.

A solvent extraction method for separating synthetic protected oligodeoxyribonucleotides was used in our laboratory based on the lipophilic property of the protecting group of 5'-OH of the oligomers. The extraction of synthetic products protected with MMTr is complete by ether or ether-chloroform (6:1 V/V) for mononucleosides, by chloroform for dinucleoside monophosphates, by dichloromethane:n-butanol (4:1 V/V) for trimer or tetramer, and is nearly complete by dichloromethane:n-butanol (2:1 V/V) for hexamer. The 5'-end phosphorylated nucleotides, oligonucleotides and their symmetrical pyrophosphates remain in water phase.