Automated high-throughput RP-HPLC-MS and SFC-MS analytical and purification platforms to support drug discovery.

In recent years, the need to accelerate drug discovery processes in the pharmaceutical industry has revived the interest of implementing automated workflows, allowing the simultaneous processing of multiple samples on global processes that are referred as High-Throughput Purification (HTP). In this work, SAPIO Laboratory Information Management System (SAPIO LIMS) has been customized at the HTP laboratories of Janssen R&D to accommodate the needs of global purification groups on several automated HTP workflows, integrating Analytical Studio™ data processing tool on multiple steps. Herein we describe the workflow details from crude analysis via RP-LC-MS or SFC-MS systems to sample redissolution and delivery to Compound Logistics (CL) in tubes ready for assay plate preparation.

Impact of the COVID-19 Pandemic on Door-to-Device Time Segments and Clinical Outcomes for STEMI Patients in Northern Taiwan.

Background: Prompt primary percutaneous coronary intervention (pPCI) is crucial for the prognosis and reduction of myocardial damage in ST-segment elevation myocardial infarction (STEMI) patients. The Coronavirus Disease 2019 (COVID-19) pandemic had multifaceted impacts on healthcare. This study assessed the effects of the pandemic on pPCI procedures and clinical outcomes in emergency STEMI patients.

Influence of the Door-to-ECG Time on the Prognosis of Patients with Acute Coronary Syndrome.

Background: The rapid acquisition of an electrocardiogram (ECG) plays a crucial role in the diagnosis and management decisions in patients with acute coronary syndrome (ACS).

Objectives: We determined the time-to-ECG acquisition, identified factors associated with timely acquisition, and evaluated the influence of time-to-ECG on in-hospital mortality.

Methods: We measured the door-to-ECG time for 903 of 2140 patients in the emergency department of Far Eastern Memorial Hospital with a diagnosis of ACS from January 1, 2016 to December 31, 2018, via a retrospective chart review.

Multiple Synthetic Routes to the Mini-Protein Omomyc and Coiled-Coil Domain Truncations.

The Myc transcription factor represents an "undruggable" target of high biological interest due to its central role in various cancers. An abbreviated form of the c-Myc protein, called Omomyc, consists of the Myc DNA-binding domain and a coiled-coil region to facilitate dimerization of the 90 amino acid polypeptide. Here we present our results to evaluate the synthesis of Omomyc using three complementary strategies: linear Fmoc solid-phase peptide synthesis (SPPS) using several advancements for difficult sequences, native chemical ligation from smaller peptide fragments, and a high-throughput bacterial expression and assay platform for rapid mutagenesis.

High-throughput purification platform in support of drug discovery.

The application of parallel synthesis is an efficient approach to explore the chemical space and to rapidly develop meaningful structure activity relationship (SAR) data for drug discovery programs. However, the effectiveness of the parallel synthesis requires a high throughput purification workflow that can process a large number of crude samples within a meaningful time frame. This paper describes a high throughput purification platform that has been adopted at Merck's Rahway research site.