Effects of TiC, Si, and Al on Combustion Synthesis of TiSiC/TiC/TiSi Composites.

The fabrication of TiSiC from TiC-containing reactant compacts was investigated by combustion synthesis in the mode of self-propagating high-temperature synthesis (SHS). The initial sample composition was formulated based on (3 - x)Ti + ySi + (2 - x)C + xTiC + zAl, with stoichiometric parameters of x from 0 to 0.7, y = 1.

Affinity Immobilization of a Bacterial Prolidase onto Metal-Ion-Chelated Magnetic Nanoparticles for the Hydrolysis of Organophosphorus Compounds.

In this study, silica-coated magnetic nanoparticles (SiMNPs) with isocyanatopropyltriethoxysilane as a metal-chelating ligand were prepared for the immobilization of His-tagged prolidase (His-PepQ). Under one-hour coupling, the enzyme-loading capacity for the Ni-functionalized SiMNPs (NiNTASiMNPs) was 1.5 mg/mg support, corresponding to about 58.

High-level expression and molecular characterization of a recombinant prolidase from NovaBlue.

Long-term use of organophosphorus (OP) compounds has become an increasing global problem and a major threat to sustainability and human health. Prolidase is a proline-specific metallopeptidase that can offer an efficient option for the degradation of OP compounds. In this study, a full-length gene from NovaBlue encoding a prolidase (PepQ) was amplified and cloned into the commercially-available vector pQE-30 to yield pQE-PepQ.