Multiple Displacement Amplification as a Solution for Low Copy Number Plasmid Sequencing.

Plasmids play a major role in bacterial adaptation to environmental stress and often contribute to antibiotic resistance and disease virulence. Although the complete sequence of each plasmid is essential for studying plasmid biology, most antibiotic resistance and virulence plasmids in are present only in a low copy number, making extraction and sequencing difficult. Long read sequencing technologies require higher concentrations of DNA to provide optimal results.

A radiomics model for preoperative prediction of brain invasion in meningioma non-invasively based on MRI: A multicentre study.

Background: Prediction of brain invasion pre-operatively rather than postoperatively would contribute to the selection of surgical techniques, predicting meningioma grading and prognosis. Here, we aimed to predict the risk of brain invasion in meningioma pre-operatively using a nomogram by incorporating radiomic and clinical features.

Methods: In this case-control study, 1728 patients from Beijing Tiantan Hospital (training cohort: n = 1070) and Lanzhou University Second Hospital (external validation cohort: n = 658) were diagnosed with meningiomas by histopathology.

Complete Genome Sequences of Two Salmonella enterica subsp. Serovar Anatum Strains Isolated from Papaya.

is a major global foodborne pathogen that causes gastroenteritis and, in some cases, death. serovar Anatum has been increasingly associated with foodborne salmonellosis outbreaks. In this report, we announce two complete genome sequences of Anatum isolated from papaya fruit.

Radiomics analysis of placenta on T2WI facilitates prediction of postpartum haemorrhage: A multicentre study.

Background: Identification of pregnancies with postpartum haemorrhage (PPH) antenatally rather than intrapartum would aid delivery planning, facilitate transfusion requirements and decrease maternal complications. MRI has been increasingly used for placenta evaluation. Here, we aim to build a nomogram incorporating both clinical and radiomic features of placenta to predict the risk for PPH in pregnancies during caesarian delivery (CD).

A Putative Microcin Amplifies Shiga Toxin 2a Production of Escherichia coli O157:H7.

O157:H7 is a foodborne pathogen implicated in various multistate outbreaks. It encodes Shiga toxin on a prophage, and Shiga toxin production is linked to phage induction. An strain, designated 0.

Design of Hierarchical Beads for Efficient Label-Free Cell Capture.

Defined hierarchical materials promise cell analysis and call for application-driven design in practical use. The further issue is to develop advanced materials and devices for efficient label-free cell capture with minimum instrumentation. Herein, the design of hierarchical beads is reported for efficient label-free cell capture.

Comparative Genomic Analysis of Virulence, Antimicrobial Resistance, and Plasmid Profiles of Dublin Isolated from Sick Cattle, Retail Beef, and Humans in the United States.

serovar Dublin is a host-adapted serotype associated with typhoidal disease in cattle. While rare in humans, it usually causes severe illness, including bacteremia. In the United States, Dublin has become one of the most multidrug-resistant (MDR) serotypes.

Genomic features of colistin resistant Escherichia coli ST69 strain harboring mcr-1 on IncHI2 plasmid from raw milk cheese in Egypt.

There is emerging evidence that food of animal origin may be responsible for the spread of multidrug resistant extraintestinal pathogenic Escherichia coli in the community. Here, we describe the emergence of colistin resistance gene, mcr-1, in a strain belonging to the dominant uropathogenic E. coli ST69 lineage.

Genome sequence analysis of 91 Salmonella Enteritidis isolates from mice caught on poultry farms in the mid 1990s.

A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995-1998.

Retrospective whole-genome sequencing analysis distinguished PFGE and drug-resistance-matched retail meat and clinical Salmonella isolates.

Non-typhoidal Salmonella is a leading cause of outbreak and sporadic-associated foodborne illnesses in the United States. These infections have been associated with a range of foods, including retail meats. Traditionally, pulsed-field gel electrophoresis (PFGE) and antibiotic susceptibility testing (AST) have been used to facilitate public health investigations of Salmonella infections.

Closed Genome Sequence of Salmonella enterica Serovar Richmond Strain CFSAN000191, Obtained with Nanopore Sequencing.

Here we report the genome sequence of Salmonella enterica serovar Richmond strain CFSAN000191, isolated from tilapia from Thailand in 2005. The genome was determined by a combination of long-read and short-read sequencing. This strain was used for source tracking in a 2012 Salmonella enterica serovar Bareilly foodborne outbreak in the United States.

First Complete Genome Sequence of Salmonella enterica subsp. enterica Serovar Worthington Strain CFSAN051295, Isolated from Pistachio.

We report here, using third-generation, single-molecule, real-time DNA sequencing, the first complete genome sequence of Salmonella enterica serovar Worthington CFSAN051295, isolated from pistachios in the United States. The genome consists of a single 4.9-Mb chromosome.

Complete Genome Sequence of a Vancomycin-Resistant Sequence Type 203 Enterococcus faecium Strain with Belonging to Complex Type 859.

In 2014, the first vancomycin-resistant (encoded by ) Enterococcus faecium isolate belonging to sequence type 203 (ST203) and complex type 859 (CT859) was detected in Denmark. In 2016, 64% of the Danish clinical E. faecium isolates belonged to ST203 and CT859.

Genes significantly associated with lineage II food isolates of Listeria monocytogenes.

Background: Listeria monocytogenes is a widespread foodborne pathogen that can cause listeriosis, a potentially fatal infection. L. monocytogenes is subdivided into four phylogenetic lineages, with the highest incidence of listeriosis occurring within lineage I followed by lineage II.

Closed Genome Sequence of Clostridium botulinum Strain CFSAN064329 (62A).

is a strictly anaerobic, Gram-positive, spore-forming bacterium that produces botulinum neurotoxin, a potent and deadly proteinaceous exotoxin. strain CFSAN064329 (62A) produces an A1 serotype/subtype botulinum neurotoxin and is frequently utilized in food challenge and detection studies. We report here the closed genome sequence of strain CFSAN064329 (62A).

Complete Genome Sequences of Two subsp. Serovar Enteritidis Strains Isolated from Egg Products in the United States.

Egg-associated salmonellosis is an important public health problem in many countries. Here, we report the genome sequences, including plasmids, of two strains of subsp. serovar Enteritidis isolated from egg products in 2012 and 2013 in the United States.

Complete Genome Sequences of Three subsp. Serovar Saintpaul Isolates Associated with a 2013 Multistate Outbreak in the United States.

In 2013, a multistate outbreak of subsp. serovar Saintpaul from cucumber caused 84 cases of salmonellosis in the United States. In this announcement, we report the complete genome sequences of three clinical Saintpaul isolates associated with the 2013 outbreak.

Complete Genome and Methylome Sequences of subsp. Serovars Typhimurium, Saintpaul, and Stanleyville from the SARA/SARB Collection.

In this announcement, we report the complete genome and methylome sequences of three strains from the SARA and SARB collection: subsp. serovar Typhimurium (SARA13), subsp. serovar Saintpaul (SARA26), and subsp.

Complete Genome and Methylome Sequences of Salmonella enterica subsp. enterica Serovar Panama (ATCC 7378) and Salmonella enterica subsp. enterica Serovar Sloterdijk (ATCC 15791).

Salmonella enterica spp. are pathogenic bacteria commonly associated with food-borne outbreaks in human and animals. Salmonella enterica spp.

Complete Genome and Methylome Sequences of Two Salmonella enterica spp.

Salmonella enterica is responsible for major foodborne outbreaks worldwide. It can cause gastroenteritis characterized by diarrhea, vomiting, and fever. Salmonella infections raise public health concerns along with consequential economic impacts.

A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection.

Burkholderia is a diverse genus of gram-negative bacteria that causes high mortality rate in humans, equines and cattle. The lack of effective therapeutic treatments poses serious public health threats. Developing insights toward host-Burkholderia spp.

Host response during Yersinia pestis infection of human bronchial epithelial cells involves negative regulation of autophagy and suggests a modulation of survival-related and cellular growth pathways.

Yersinia pestis (Yp) causes the re-emerging disease plague, and is classified by the CDC and NIAID as a highest priority (Category A) pathogen. Currently, there is no approved human vaccine available and advances in early diagnostics and effective therapeutics are urgently needed. A deep understanding of the mechanisms of host response to Yp infection can significantly advance these three areas.