A fully automated effervescence assisted dispersive liquid-liquid microextraction based on a stepwise injection system. Determination of antipyrine in saliva samples.

A first attempt to automate the effervescence assisted dispersive liquid-liquid microextraction (EA-DLLME) has been reported. The method is based on the aspiration of a sample and all required aqueous reagents into the stepwise injection analysis (SWIA) manifold, followed by simultaneous counterflow injection of the extraction solvent (dichloromethane), the mixture of the effervescence agent (0.5 mol L(-1) Na2CO3) and the proton donor solution (1 mol L(-1) CH3COOH).

Determination of antipyrine in saliva using the dispersive liquid-liquid microextraction based on a stepwise injection system.

A fully automated stepwise injection spectrophotometric method for determination of antipyrine in saliva (agent for non-invasive assessment of the activity of the drug metabolizing system in hepatocytes) has been developed. The method is based on the antipyrine derivatization by nitrite-ion dispersive liquid-liquid microextraction (DLLME) of formed 4-nitrosoantipyrine with subsequent UV-vis spectrophotometric detection. Under optimal experimental conditions (0.