Publications by authors named "Ksenia A Brovina"
Article Synopsis
- The study found a new anticoagulant protein from the saliva of medicinal leeches, which showed strong ability to suppress blood coagulation.
- This protein was tested against commonly used anticoagulants like hirudin and demonstrated superior inhibition in certain assays, though hirudin had better results in direct thrombin inhibition tests.
- The newly identified protein shares similarities with antistatin, indicating it could potentially inhibit other proteins involved in blood coagulation.
Article Synopsis
- Recombinant proteins from E. coli often have endotoxin contamination, which complicates their use.
- Two methods were tested to create E. coli strains with lower lipopolysaccharide (LPS) levels: knocking out genes in the LPS biosynthesis pathway and increasing YciM protein expression.
- Both approaches successfully reduced endotoxin levels in the purified eGFP samples.