Regulatory T lymphocytes and transforming growth factor beta in epithelial ovarian tumors-prognostic significance.

Background: Regulatory T lymphocytes (Treg) are characterized by the presence of CD4+ surface antigen. Today the transcription factor FOXP3 is considered to be the most specific marker of Treg cells. The aim of the study was to estimate the percentage of Treg in peripheral blood and the tissue of the epithelial ovarian tumor and blood serum TGF-beta concentrations and relationships between them.

8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women.

Objectives: In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human uterine estrogen-dependent tissue cancers.

Material And Methods: In order to estimate the level of oxidative damage, 8-oxo-7,8-dihydroguanine was determined directly in cells of tissue microscope slides using OxyDNA Assay Kit, Fluorometric. Cells were investigated under confocal microscope.

Estrogen and progesterone receptor expression in HPV-positive and HPV-negative cervical carcinomas.

Human papillomavirus (HPV) is widely accepted as the main cause of cervical cancer. However, the presence of HPV DNA does not inescapably lead to the development of the cancerous phenotype of the infected cell. Therefore, it is considered that the induction of full cancerous expression of HPV requires additional cofactors.

PARP-1 activity in normal and cancerous human endometrium and its relationship with quantity of abasic sites (AP).

Objectives: Poly (ADP-ribose) polymerase (PARP-1) is involved in the processes of DNA repair contributing to the maintenance of genomic stability. Recent data suggest that polymerase is involved in the development of endometrial adenocarcinomas and more advanced tumors displaying lowest enzyme protein expression. Data on PARP-1 activity regarding carcinogenesis in human endometrium are scarce.

[Vesicovaginal fistulas--current therapeutical strategy].

Objectives: Clinical evaluation of Latzko operation in primary and recurrent vesicovaginal fistulas and an update on current therapeutical strategy.

Material And Methods: Evaluation of clinical efficacy has been performed in a group of 5 patients with confirmed vesicovaginal fistulas and in 1 woman with urethrovaginal fistula. Prior to the operation, four patients had undergone eight fistula operations in our department with unsuccessful outcome.

Is global DNA methylation in sporadic uterine adenocarcinomas in women a result of histological and clinical tumor advancement?

Aim: To find out the relationship, if any between the extent of the overall genomic DNA methylation, and clinical and pathological features of the sporadic endometrial adenocarcinomas in women.

Material: Genomic DNA was isolated from 44 primary uterine cancer tissue specimens. There were eight G1, 24 G2 and twelve G3 tumors.

[Conservative treatment of cervical pregnancy in women: a review of the literature with case report].

Diagnosis of cervical pregnancy depends on ultrasonographic image of the gestational sac within the cervix, rather than histopathologic examination. Diagnosis of a cervical pregnancy may be confirmed if the placenta and the entire chorionic sac containing a live fetus are located below the internal os. Majority of patients with cervical pregnancies are young women who wish to stay fertile and have the possibility to bear children.

[Apurinic/apyrimidinic sites (AP)--stress oxidative marker--in normal and neoplastic human endometrium].

Objectives: To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement.

Material And Methods: AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 noncancerous endometrial samples using Oxidative DNA Damage Kit Quantitative (Kamiya Biomedical Company).

Results: Mean AP sites in noncancerous endometrium was 6.

Changing trends in the surgical treatment of female stress urinary incontinence--twenty two years observation.

Objectives: The aim of this study was to analyze the changing trends in surgical treatment of female urinary incontinence (UI).

Material And Methods: Medical records of all women admitted to II Department of Gynecology from 1985 to 2006 were analyzed in order to find out how the female SUI treatment changed over these years.

Results: During analyzed time 36819 patients were hospitalized in our Department and 77.

[DNA adducts in human female genital organs].

DNA adducts, one of genetic damages markers, precede and finally can lead to oncogenic mutations. They appear in genome as a result of DNA bases damages caused by various and numerous environmental factors eg. ultraviolet light, ionic radiation, toxins and also endogenic substances, for example estrogens.

Activity of matrix metalloproteinase-2 and -9 and contents of their tissue inhibitors in uterine leiomyoma and corresponding myometrium.

Background And Aim: Matrix metalloproteinase-2 and -9 (MMP-2 and -9) are proteolytic enzymes degrading extracellular matrix proteins, mainly collagen type IV. Recent reports show that these proteases may be implicated in the growth of uterine leiomyoma. The aim of the present study was to evaluate the activity of MMP-2 and MMP-9, the contents of their tissue inhibitors (TIMP-1 and TIMP-2) and the immunolocalization of collagen type IV in uterine leiomyoma and corresponding myometrium.

Immunohistochemical detection of hMLH1 and hMSH2 proteins in vulvar carcinoma.

The immunohistochemical (IHC) detection of MMR proteins is an accurate and rapid method to predict the presence of defective DNA MMR genes. MMR protein expression could also serve as a prognostic indicator of human cancers. The results of many studies demonstrate the usefulness of IHC tests with monoclonal antibodies MSH2 and MLH1 in screening the microsatellite sequence instability within both spontaneous and hereditary malignant neoplasms.

Global DNA methylation in relation to hMLH1 and hMSH2 protein immunoreactivity in sporadic human endometrial carcinomas.

Overall DNA methylation status was studied in a group of 28 sporadic human endometrial carcinomas (ECs) using the [32P]-postlabeling technique. Moreover, expression of the DNA mismatch repair proteins (hMLH1 and hMSH2) was investigated in ECs using immunohistochemistry. Mean 5-methyldeoxycytosine (m5dC) content in the studied group was 3.

[TVT procedure--the critical analysis of clinical effectiveness and complications among first 100 cases].

Rationale: Since its clinical debut in 1996 TVT procedure has been offering an excellent clinical tool both for patients and surgeons to treat SUI suffering women. Since the learning curve is an important factor influencing the rate of surgical complications as well as the clinical outcome of any new surgical technique we decided to analyse the first 100 cases of SUI women treated with TVT technique.

Aim Of The Study: To analyse clinical effectiveness and complications among first 100 consecutive cases of TVT procedures.

Serum concentrations of markers of type I collagen metabolism in women taking monophasic oral contraceptives.

Objectives: To investigate serum concentrations of the carboxy-terminal propeptide of type I procollagen (PICP) and cross-linked carboxy-terminal telopeptide of type I collagen (ICTP), reflecting the rate of synthesis and degradation of the parent collagen respectively, in women using monophasic oral contraceptives.

Study Design: PICP and ICTP were estimated in 60 women taking 20 micrograms of ethinyl estradiol (EE) + 150 micrograms of desogestrel (DSG) or 30 micrograms of EE + 150 micrograms DSG over six months.

Results: Mean PICP concentration decreased in women receiving 20 micrograms of EE + 150 infinity g of DSG after three cycles of administration.

Is diminished pubocervical fascia collagen content a risk factor for failure of surgical management of genuine stress urinary incontinence in women?

Objective: The assessment of relationship between pubocervical collagen content and clinical results of surgical treatment of genuine stress urinary incontinence (GSUI) in women.

Methods: Twenty-four women treated for genuine stress urinary incontinence were included into the study. All women underwent the same surgical procedure.